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Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio)

Abstract

Nanoparticles (NPs) can be ingested by organisms, and NPs with antimicrobial properties may disrupt beneficial endogenous microbial communities and affect organism health. Zebrafish were fed diets containing Cu-NPs or Ag-NPs (500 mg kg{sup −1} food), or an appropriate control for 14 d. Intestinal epithelium integrity was examined by transmission electron microscopy, and microbial community structure within the intestine was assessed by denaturing gradient gel electrophoresis (DGGE) of partial 16S rRNA. No lesions were observed in intestinal epithelia; however, presence of NPs in diets changed intestinal microbial community structure. In particular, some beneficial bacterial strains (e.g., Cetobacterium somerae) were suppressed to non-detectable levels by Cu-NP exposure, and two unidentified bacterial clones from the Firmicutes phylum were sensitive (not detected) to Cu, but were present in Ag and control fish. Unique changes in zebrafish microbiome caused by exposure to Ag-NP and Cu-NP indicate that NP ingestion could affect digestive system function and organism health. -- Highlights: ► Zebrafish ingest Cu- and Ag-nanoparticles (NPs) in diet. ► No effect of Cu-NPs or Ag-NPs on intestinal epithelial integrity. ► Cu-NPs and Ag-NPs alter endogenous microbiota of zebrafish. -- Dietary exposure to manufactured Cu- and Ag-nanoparticles caused unique changes in endogenous gut microbiota in zebrafish  More>>
Publication Date:
Mar 15, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Environmental Pollution (1987); Journal Volume: 174; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; DIET; ELECTROPHORESIS; EPITHELIUM; FOOD; GELS; INGESTION; INTESTINES; METALS; NANOSTRUCTURES; TRANSMISSION ELECTRON MICROSCOPY
OSTI ID:
22240483
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0269-7491; CODEN: ENPOEK; Other: PII: S0269-7491(12)00495-2; TRN: GB14R6350061612
Availability:
Available from http://dx.doi.org/10.1016/j.envpol.2012.11.017
Submitting Site:
INIS
Size:
page(s) 157-163
Announcement Date:
Jun 27, 2014

Citation Formats

Merrifield, Daniel L., Shaw, Benjamin J., Harper, Glenn M., Saoud, Imad P., Davies, Simon J., Handy, Richard D., Henry, Theodore B., E-mail: ted.henry@plymouth.ac.uk [School of Biomedical and Biological Sciences, Plymouth University, 401 Davy Building, Drake Circus, Plymouth PL4 8AA, Devon (United Kingdom), Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States), and Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, TN (United States)]. Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio). United Kingdom: N. p., 2013. Web. doi:10.1016/J.ENVPOL.2012.11.017.
Merrifield, Daniel L., Shaw, Benjamin J., Harper, Glenn M., Saoud, Imad P., Davies, Simon J., Handy, Richard D., Henry, Theodore B., E-mail: ted.henry@plymouth.ac.uk [School of Biomedical and Biological Sciences, Plymouth University, 401 Davy Building, Drake Circus, Plymouth PL4 8AA, Devon (United Kingdom), Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States), & Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, TN (United States)]. Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio). United Kingdom. https://doi.org/10.1016/J.ENVPOL.2012.11.017
Merrifield, Daniel L., Shaw, Benjamin J., Harper, Glenn M., Saoud, Imad P., Davies, Simon J., Handy, Richard D., Henry, Theodore B., E-mail: ted.henry@plymouth.ac.uk [School of Biomedical and Biological Sciences, Plymouth University, 401 Davy Building, Drake Circus, Plymouth PL4 8AA, Devon (United Kingdom), Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States), and Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, TN (United States)]. 2013. "Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio)." United Kingdom. https://doi.org/10.1016/J.ENVPOL.2012.11.017.
@misc{etde_22240483,
title = {Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio)}
author = {Merrifield, Daniel L., Shaw, Benjamin J., Harper, Glenn M., Saoud, Imad P., Davies, Simon J., Handy, Richard D., Henry, Theodore B., E-mail: ted.henry@plymouth.ac.uk [School of Biomedical and Biological Sciences, Plymouth University, 401 Davy Building, Drake Circus, Plymouth PL4 8AA, Devon (United Kingdom), Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN (United States), and Department of Forestry, Wildlife and Fisheries, University of Tennessee, Knoxville, TN (United States)]}
abstractNote = {Nanoparticles (NPs) can be ingested by organisms, and NPs with antimicrobial properties may disrupt beneficial endogenous microbial communities and affect organism health. Zebrafish were fed diets containing Cu-NPs or Ag-NPs (500 mg kg{sup −1} food), or an appropriate control for 14 d. Intestinal epithelium integrity was examined by transmission electron microscopy, and microbial community structure within the intestine was assessed by denaturing gradient gel electrophoresis (DGGE) of partial 16S rRNA. No lesions were observed in intestinal epithelia; however, presence of NPs in diets changed intestinal microbial community structure. In particular, some beneficial bacterial strains (e.g., Cetobacterium somerae) were suppressed to non-detectable levels by Cu-NP exposure, and two unidentified bacterial clones from the Firmicutes phylum were sensitive (not detected) to Cu, but were present in Ag and control fish. Unique changes in zebrafish microbiome caused by exposure to Ag-NP and Cu-NP indicate that NP ingestion could affect digestive system function and organism health. -- Highlights: ► Zebrafish ingest Cu- and Ag-nanoparticles (NPs) in diet. ► No effect of Cu-NPs or Ag-NPs on intestinal epithelial integrity. ► Cu-NPs and Ag-NPs alter endogenous microbiota of zebrafish. -- Dietary exposure to manufactured Cu- and Ag-nanoparticles caused unique changes in endogenous gut microbiota in zebrafish Danio rerio.}
doi = {10.1016/J.ENVPOL.2012.11.017}
journal = []
volume = {174}
journal type = {AC}
place = {United Kingdom}
year = {2013}
month = {Mar}
}