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Relationship to reducing sugar production and scanning electron microscope structure to pretreated hemp hurd biomass (Cannabis sativa)

Abstract

Lignocellulosic biomass is a highly rigid and recalcitrant structure which requires pretreatment to loosen chemical bonds to make accessible monomeric sugars for biofuel production. In this study, locally available biomass, that is hemp (Cannabis sativa), a low cost feedstock for ethanol production, has been used for the production of fermentable sugars. Hemp hurd biomass (HHB) was exposed to five different pretreatments which included dilute acid (H{sub 2}SO{sub 4}), alkaline (NaOH), alkaline peroxide, hot water and one stage dilute acid (H{sub 2}SO{sub 4}). Different pretreatments resulted in loosening and degradation of HHB structure thus facilitating enzymatic saccharification at optimized parameters (pH–4.8 and 50 °C). The changes in the reactive groups (hydroxyl or acetyl) of the HHB were confirmed by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Scanning electron microscopy (SEM) was employed to characterize the surface morphology of untreated and treated HHB. Finally, enzymatic saccharification demonstrated maximum yield of total sugars (743 mg g{sup −1}) that are suitable for biofuel production. -- Highlights: • Hemp hurd biomass (HHB) was used for producing fermentable sugars. • Alkaline pretreatment resulted in loosening and degradation of hemp structure. • Pretreated HHB was characterized using FTIR studies. • SEM studies evaluated the opening of  More>>
Publication Date:
Nov 15, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Biomass and Bioenergy; Journal Volume: 58; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 09 BIOMASS FUELS; BIOFUELS; BIOMASS; CELLULASE; CELLULOSE; CHEMICAL BONDS; ETHANOL; FOURIER TRANSFORMATION; HOT WATER; INFRARED SPECTRA; PEROXIDES; SACCHARIFICATION; SACCHAROSE; SCANNING ELECTRON MICROSCOPY; SODIUM HYDROXIDES; SULFURIC ACID
OSTI ID:
22233959
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0961-9534; CODEN: BMSBEO; Other: PII: S0961-9534(13)00309-7; TRN: GB14R2298055034
Availability:
Available from http://dx.doi.org/10.1016/j.biombioe.2013.06.006
Submitting Site:
INIS
Size:
page(s) 180-187
Announcement Date:
Jun 05, 2014

Citation Formats

Abraham, Reinu E., Barrow, Colin J., and Puri, Munish. Relationship to reducing sugar production and scanning electron microscope structure to pretreated hemp hurd biomass (Cannabis sativa). United Kingdom: N. p., 2013. Web. doi:10.1016/J.BIOMBIOE.2013.06.006.
Abraham, Reinu E., Barrow, Colin J., & Puri, Munish. Relationship to reducing sugar production and scanning electron microscope structure to pretreated hemp hurd biomass (Cannabis sativa). United Kingdom. doi:10.1016/J.BIOMBIOE.2013.06.006.
Abraham, Reinu E., Barrow, Colin J., and Puri, Munish. 2013. "Relationship to reducing sugar production and scanning electron microscope structure to pretreated hemp hurd biomass (Cannabis sativa)." United Kingdom. doi:10.1016/J.BIOMBIOE.2013.06.006. https://www.osti.gov/servlets/purl/10.1016/J.BIOMBIOE.2013.06.006.
@misc{etde_22233959,
title = {Relationship to reducing sugar production and scanning electron microscope structure to pretreated hemp hurd biomass (Cannabis sativa)}
author = {Abraham, Reinu E., Barrow, Colin J., and Puri, Munish}
abstractNote = {Lignocellulosic biomass is a highly rigid and recalcitrant structure which requires pretreatment to loosen chemical bonds to make accessible monomeric sugars for biofuel production. In this study, locally available biomass, that is hemp (Cannabis sativa), a low cost feedstock for ethanol production, has been used for the production of fermentable sugars. Hemp hurd biomass (HHB) was exposed to five different pretreatments which included dilute acid (H{sub 2}SO{sub 4}), alkaline (NaOH), alkaline peroxide, hot water and one stage dilute acid (H{sub 2}SO{sub 4}). Different pretreatments resulted in loosening and degradation of HHB structure thus facilitating enzymatic saccharification at optimized parameters (pH–4.8 and 50 °C). The changes in the reactive groups (hydroxyl or acetyl) of the HHB were confirmed by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Scanning electron microscopy (SEM) was employed to characterize the surface morphology of untreated and treated HHB. Finally, enzymatic saccharification demonstrated maximum yield of total sugars (743 mg g{sup −1}) that are suitable for biofuel production. -- Highlights: • Hemp hurd biomass (HHB) was used for producing fermentable sugars. • Alkaline pretreatment resulted in loosening and degradation of hemp structure. • Pretreated HHB was characterized using FTIR studies. • SEM studies evaluated the opening of fiber bundles in pretreatment, thereby increasing cellulose access to enzymes. • Enzymatic saccharification of pretreated HHB demonstrated maximum yield of reducing sugars.}
doi = {10.1016/J.BIOMBIOE.2013.06.006}
journal = {Biomass and Bioenergy}
volume = {58}
journal type = {AC}
place = {United Kingdom}
year = {2013}
month = {Nov}
}