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Aerobic degradation of 4-nitroaniline (4-NA) via novel degradation intermediates by Rhodococcus sp. strain FK48

Abstract

Highlights: • This study reports isolation of a novel bacterium capable of mineralizing 4-nitroaniline (4-NA). • This bacterium has been identified as Rhodococcus sp. strain FK48. • Strain FK48 degrades 4-NA via a novel aerobic degradation pathway that involves 4-AP and 1,2,4-BT. • Subsequent degradation proceeds via ring fission and formation of maleylacetate. • This is the first report showing elucidation of catabolic pathway for microbial degradation 4-NA. -- Abstract: An aerobic strain, Rhodococcus sp. strain FK48, capable of growing on 4-nitroaniline (4-NA) as the sole source of carbon, nitrogen, and energy has been isolated from enrichment cultures originating from contaminated soil samples. During growth studies with non- induced cells of FK48 catalyzed sequential denitrification (release of NO{sub 2} substituent) and deamination (release of NH{sub 2} substituent) of 4-NA. However, none of the degradation intermediates could be identified with growth studies. During resting cell studies, 4-NA-induced cells of strain FK48 transformed 4-NA via a previously unknown pathway which involved oxidative hydroxylation leading to formation of 4-aminophenol (4-AP). Subsequent degradation involved oxidated deamination of 4-AP and formation of 1,2,4-benzenetriol (BT) as the major identified terminal aromatic intermediate. Identification of these intermediates was ascertained by HPLC, and GC–MS analyses of the culture  More>>
Publication Date:
Jun 15, 2013
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Hazardous Materials; Journal Volume: 254-255; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
36 MATERIALS SCIENCE; 60 APPLIED LIFE SCIENCES; BIODEGRADATION; CARBON; DEAMINATION; DENITRIFICATION; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; NITROGEN; NITROGEN DIOXIDE; RHODOCOCCUS; SOILS; STRAINS
OSTI ID:
22230007
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0304-3894; CODEN: JHMAD9; Other: PII: S0304-3894(13)00197-0; TRN: NL14R5357051092
Availability:
Available from http://dx.doi.org/10.1016/j.jhazmat.2013.03.021
Submitting Site:
NLN
Size:
page(s) 72-78
Announcement Date:
May 22, 2014

Citation Formats

Khan, Fazlurrahman, Pandey, Janmejay, Vikram, Surendra, Pal, Deepika, and Cameotra, Swaranjit Singh, E-mail: ssc@imtech.res.in. Aerobic degradation of 4-nitroaniline (4-NA) via novel degradation intermediates by Rhodococcus sp. strain FK48. Netherlands: N. p., 2013. Web. doi:10.1016/J.JHAZMAT.2013.03.021.
Khan, Fazlurrahman, Pandey, Janmejay, Vikram, Surendra, Pal, Deepika, & Cameotra, Swaranjit Singh, E-mail: ssc@imtech.res.in. Aerobic degradation of 4-nitroaniline (4-NA) via novel degradation intermediates by Rhodococcus sp. strain FK48. Netherlands. doi:10.1016/J.JHAZMAT.2013.03.021.
Khan, Fazlurrahman, Pandey, Janmejay, Vikram, Surendra, Pal, Deepika, and Cameotra, Swaranjit Singh, E-mail: ssc@imtech.res.in. 2013. "Aerobic degradation of 4-nitroaniline (4-NA) via novel degradation intermediates by Rhodococcus sp. strain FK48." Netherlands. doi:10.1016/J.JHAZMAT.2013.03.021. https://www.osti.gov/servlets/purl/10.1016/J.JHAZMAT.2013.03.021.
@misc{etde_22230007,
title = {Aerobic degradation of 4-nitroaniline (4-NA) via novel degradation intermediates by Rhodococcus sp. strain FK48}
author = {Khan, Fazlurrahman, Pandey, Janmejay, Vikram, Surendra, Pal, Deepika, and Cameotra, Swaranjit Singh, E-mail: ssc@imtech.res.in}
abstractNote = {Highlights: • This study reports isolation of a novel bacterium capable of mineralizing 4-nitroaniline (4-NA). • This bacterium has been identified as Rhodococcus sp. strain FK48. • Strain FK48 degrades 4-NA via a novel aerobic degradation pathway that involves 4-AP and 1,2,4-BT. • Subsequent degradation proceeds via ring fission and formation of maleylacetate. • This is the first report showing elucidation of catabolic pathway for microbial degradation 4-NA. -- Abstract: An aerobic strain, Rhodococcus sp. strain FK48, capable of growing on 4-nitroaniline (4-NA) as the sole source of carbon, nitrogen, and energy has been isolated from enrichment cultures originating from contaminated soil samples. During growth studies with non- induced cells of FK48 catalyzed sequential denitrification (release of NO{sub 2} substituent) and deamination (release of NH{sub 2} substituent) of 4-NA. However, none of the degradation intermediates could be identified with growth studies. During resting cell studies, 4-NA-induced cells of strain FK48 transformed 4-NA via a previously unknown pathway which involved oxidative hydroxylation leading to formation of 4-aminophenol (4-AP). Subsequent degradation involved oxidated deamination of 4-AP and formation of 1,2,4-benzenetriol (BT) as the major identified terminal aromatic intermediate. Identification of these intermediates was ascertained by HPLC, and GC–MS analyses of the culture supernatants. 4-NA-induced cells of strain FK48 showed positive activity for 1,2,4-benzenetriol dioxygenase in spectrophotometric assay. This is the first conclusive study on aerobic microbial degradation of 4-NA and elucidation of corresponding metabolic pathway.}
doi = {10.1016/J.JHAZMAT.2013.03.021}
journal = {Journal of Hazardous Materials}
volume = {254-255}
journal type = {AC}
place = {Netherlands}
year = {2013}
month = {Jun}
}