Abstract
Purpose: To investigate the relationship between human papillomavirus (HPV) genotype and outcome after radiation therapy and intrinsic radiosensitivity. Methods and Materials: HPV genotyping was performed on cervix biopsies by polymerase chain reaction using SPF-10 broad-spectrum primers, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse hybridization line probe assay (LiPA{sub 25}) (version 1) (n=202). PapilloCheck and quantitative reverse transcription-polymerase chain reaction were used to genotype cervix cancer cell lines (n=16). Local progression-free survival after radiation therapy alone was assessed using log-rank and Cox proportionate hazard analyses. Intrinsic radiosensitivity was measured as surviving fraction at 2 Gy (SF2) using clonogenic assays. Results: Of the 202 tumors, 107 (53.0%) were positive for HPV16, 29 (14.4%) for HPV18, 9 (4.5%) for HPV45, 23 (11.4%) for other HPV genotypes, and 22 (10.9%) were negative; 11 (5.5%) contained multiple genotypes, and 1 tumor was HPV X (0.5%). In 148 patients with outcome data, those with HPVα9-positive tumors had better local progression-free survival compared with α7 patients in univariate (P<.004) and multivariate (hazard ratio 1.54, 95% confidence interval 1.11-1.76, P=.021) analyses. There was no difference in the median SF2 of α9 and α7 cervical tumors (n=63). In the cell lines, 9 were α7 and 4
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Hall, John S.;
Iype, Rohan;
Armenoult, Lucile S.C.;
[1]
Taylor, Janet;
[1]
Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)];
Miller, Crispin J.;
[2]
Davidson, Susan;
[3]
Sanjose, Silvia de;
Bosch, Xavier;
[4]
Stern, Peter L.;
[5]
West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk
[1]
- Translational Radiobiology Group, Institute of Cancer Sciences, Manchester Academic Health Science Centre, University of Manchester, Manchester (United Kingdom)
- Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)
- Christie National Health Service Foundation Trust, Manchester (United Kingdom)
- Cancer Epidemiology Research Program, Catalan Institute of Oncology, L'Hospitalet de Llobregat (Spain)
- Immunology Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)
Citation Formats
Hall, John S., Iype, Rohan, Armenoult, Lucile S.C., Taylor, Janet, Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)], Miller, Crispin J., Davidson, Susan, Sanjose, Silvia de, Bosch, Xavier, Stern, Peter L., and West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk.
Poor Prognosis Associated With Human Papillomavirus α7 Genotypes in Cervical Carcinoma Cannot Be Explained by Intrinsic Radiosensitivity.
United States: N. p.,
2013.
Web.
doi:10.1016/J.IJROBP.2012.11.030.
Hall, John S., Iype, Rohan, Armenoult, Lucile S.C., Taylor, Janet, Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)], Miller, Crispin J., Davidson, Susan, Sanjose, Silvia de, Bosch, Xavier, Stern, Peter L., & West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk.
Poor Prognosis Associated With Human Papillomavirus α7 Genotypes in Cervical Carcinoma Cannot Be Explained by Intrinsic Radiosensitivity.
United States.
https://doi.org/10.1016/J.IJROBP.2012.11.030
Hall, John S., Iype, Rohan, Armenoult, Lucile S.C., Taylor, Janet, Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)], Miller, Crispin J., Davidson, Susan, Sanjose, Silvia de, Bosch, Xavier, Stern, Peter L., and West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk.
2013.
"Poor Prognosis Associated With Human Papillomavirus α7 Genotypes in Cervical Carcinoma Cannot Be Explained by Intrinsic Radiosensitivity."
United States.
https://doi.org/10.1016/J.IJROBP.2012.11.030.
@misc{etde_22224430,
title = {Poor Prognosis Associated With Human Papillomavirus α7 Genotypes in Cervical Carcinoma Cannot Be Explained by Intrinsic Radiosensitivity}
author = {Hall, John S., Iype, Rohan, Armenoult, Lucile S.C., Taylor, Janet, Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)], Miller, Crispin J., Davidson, Susan, Sanjose, Silvia de, Bosch, Xavier, Stern, Peter L., and West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk}
abstractNote = {Purpose: To investigate the relationship between human papillomavirus (HPV) genotype and outcome after radiation therapy and intrinsic radiosensitivity. Methods and Materials: HPV genotyping was performed on cervix biopsies by polymerase chain reaction using SPF-10 broad-spectrum primers, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse hybridization line probe assay (LiPA{sub 25}) (version 1) (n=202). PapilloCheck and quantitative reverse transcription-polymerase chain reaction were used to genotype cervix cancer cell lines (n=16). Local progression-free survival after radiation therapy alone was assessed using log-rank and Cox proportionate hazard analyses. Intrinsic radiosensitivity was measured as surviving fraction at 2 Gy (SF2) using clonogenic assays. Results: Of the 202 tumors, 107 (53.0%) were positive for HPV16, 29 (14.4%) for HPV18, 9 (4.5%) for HPV45, 23 (11.4%) for other HPV genotypes, and 22 (10.9%) were negative; 11 (5.5%) contained multiple genotypes, and 1 tumor was HPV X (0.5%). In 148 patients with outcome data, those with HPVα9-positive tumors had better local progression-free survival compared with α7 patients in univariate (P<.004) and multivariate (hazard ratio 1.54, 95% confidence interval 1.11-1.76, P=.021) analyses. There was no difference in the median SF2 of α9 and α7 cervical tumors (n=63). In the cell lines, 9 were α7 and 4 α9 positive and 3 negative. There was no difference in SF2 between α9 and α7 cell lines (n=14). Conclusion: The reduced radioresponsiveness of α7 cervical tumors is not related to intrinsic radiosensitivity.}
doi = {10.1016/J.IJROBP.2012.11.030}
journal = []
issue = {5}
volume = {85}
journal type = {AC}
place = {United States}
year = {2013}
month = {Apr}
}
title = {Poor Prognosis Associated With Human Papillomavirus α7 Genotypes in Cervical Carcinoma Cannot Be Explained by Intrinsic Radiosensitivity}
author = {Hall, John S., Iype, Rohan, Armenoult, Lucile S.C., Taylor, Janet, Applied Computational Biology and Bioinformatics Group, Paterson Institute for Cancer Research, Manchester (United Kingdom)], Miller, Crispin J., Davidson, Susan, Sanjose, Silvia de, Bosch, Xavier, Stern, Peter L., and West, Catharine M.L., E-mail: Catharine.West@manchester.ac.uk}
abstractNote = {Purpose: To investigate the relationship between human papillomavirus (HPV) genotype and outcome after radiation therapy and intrinsic radiosensitivity. Methods and Materials: HPV genotyping was performed on cervix biopsies by polymerase chain reaction using SPF-10 broad-spectrum primers, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse hybridization line probe assay (LiPA{sub 25}) (version 1) (n=202). PapilloCheck and quantitative reverse transcription-polymerase chain reaction were used to genotype cervix cancer cell lines (n=16). Local progression-free survival after radiation therapy alone was assessed using log-rank and Cox proportionate hazard analyses. Intrinsic radiosensitivity was measured as surviving fraction at 2 Gy (SF2) using clonogenic assays. Results: Of the 202 tumors, 107 (53.0%) were positive for HPV16, 29 (14.4%) for HPV18, 9 (4.5%) for HPV45, 23 (11.4%) for other HPV genotypes, and 22 (10.9%) were negative; 11 (5.5%) contained multiple genotypes, and 1 tumor was HPV X (0.5%). In 148 patients with outcome data, those with HPVα9-positive tumors had better local progression-free survival compared with α7 patients in univariate (P<.004) and multivariate (hazard ratio 1.54, 95% confidence interval 1.11-1.76, P=.021) analyses. There was no difference in the median SF2 of α9 and α7 cervical tumors (n=63). In the cell lines, 9 were α7 and 4 α9 positive and 3 negative. There was no difference in SF2 between α9 and α7 cell lines (n=14). Conclusion: The reduced radioresponsiveness of α7 cervical tumors is not related to intrinsic radiosensitivity.}
doi = {10.1016/J.IJROBP.2012.11.030}
journal = []
issue = {5}
volume = {85}
journal type = {AC}
place = {United States}
year = {2013}
month = {Apr}
}