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Lethal action of canavanine in Escherichia coli; Etude de l'action lethale de la canavanine sur Escherichia coli

Thesis/Dissertation:

Abstract

We studied the action of canavanine, analog of arginine, on the loss of bacteria viability; macromolecular synthesis (proteins, RNA and DNA) and DNA structure. We used bacteria because among different kinds of cells (ascitic tumor cells, erythrocytes, reticulocytes) they are the only ones which show the best capability of concentrating canavanine. This compound penetrates bacteria cells by active transportation uptake. Canavanine causes an irreversible inhibition of the cell division of bacteria. The loss of viability is connected to the incorporation in proteins of canavanine instead of arginine. Canavanine stimulates RNA synthesis in bacteria, strains RC{sup Str} arg{sup -}, starved of arginine, but it inhibits the incorporation of uracil, UMP and UDP in strains RC{sup str} arg{sup -}. Canavanine allows the completion of pre-initiated DNA chains; this result is similar to that observed of starved amino acid bacteria. The DNA of bacteria incubated with canavanine shows a lesion which results in the breaking of DNA. Part of the DNA does not sediment at 10 000 xg with the membranes. This DNA does not sediment either at 150 000 xg in alkaline sucrose gradient. Our results are consistent with the hypothesis that canavanine death is due to this breaking of the DNA.  More>>
Publication Date:
Jan 04, 1972
Product Type:
Thesis/Dissertation
Report Number:
CEA-R-4305
Resource Relation:
Other Information: 59 refs.; Available from the INIS Liaison Officer for France, see the 'INIS contacts' section of the INIS website for current contact and E-mail addresses: http://www.iaea.org/inis/Contacts/; These Docteur Es-Sciences, mention Sciences Appliquees
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; ARGININE; CELL DIVISION; DNA; DNA DAMAGES; DNA REPLICATION; ERYTHROCYTES; ESCHERICHIA COLI; GROWTH; INHIBITION; PROTEIN STRUCTURE; RADIOBIOLOGY; RNA; UMP; URACILS; VIABILITY
OSTI ID:
22218680
Research Organizations:
Faculte des Sciences, Universite de Paris 7 (France); Commissariat a l'Energie Atomique, Centre d'Etudes Nucleaires de Saclay, Institut National dEs Sciences et Techniques Nucleaires, Service de Biochimie, Laboratoire de Radiobiologie (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
TRN: FR1400761039646
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
85 page(s)
Announcement Date:
Apr 21, 2014

Thesis/Dissertation:

Citation Formats

Simonnet, Gerard Marc. Lethal action of canavanine in Escherichia coli; Etude de l'action lethale de la canavanine sur Escherichia coli. France: N. p., 1972. Web.
Simonnet, Gerard Marc. Lethal action of canavanine in Escherichia coli; Etude de l'action lethale de la canavanine sur Escherichia coli. France.
Simonnet, Gerard Marc. 1972. "Lethal action of canavanine in Escherichia coli; Etude de l'action lethale de la canavanine sur Escherichia coli." France.
@misc{etde_22218680,
title = {Lethal action of canavanine in Escherichia coli; Etude de l'action lethale de la canavanine sur Escherichia coli}
author = {Simonnet, Gerard Marc}
abstractNote = {We studied the action of canavanine, analog of arginine, on the loss of bacteria viability; macromolecular synthesis (proteins, RNA and DNA) and DNA structure. We used bacteria because among different kinds of cells (ascitic tumor cells, erythrocytes, reticulocytes) they are the only ones which show the best capability of concentrating canavanine. This compound penetrates bacteria cells by active transportation uptake. Canavanine causes an irreversible inhibition of the cell division of bacteria. The loss of viability is connected to the incorporation in proteins of canavanine instead of arginine. Canavanine stimulates RNA synthesis in bacteria, strains RC{sup Str} arg{sup -}, starved of arginine, but it inhibits the incorporation of uracil, UMP and UDP in strains RC{sup str} arg{sup -}. Canavanine allows the completion of pre-initiated DNA chains; this result is similar to that observed of starved amino acid bacteria. The DNA of bacteria incubated with canavanine shows a lesion which results in the breaking of DNA. Part of the DNA does not sediment at 10 000 xg with the membranes. This DNA does not sediment either at 150 000 xg in alkaline sucrose gradient. Our results are consistent with the hypothesis that canavanine death is due to this breaking of the DNA. (author) [French] Dans le present travail, on a etudie l'action de la canavanine, analogue structural de l'arginine, sur la viabilite des bacteries, les syntheses macromoleculaires (proteines, ARN et ADN) et la structure de l'ADN. Les bacteries ont ete utilisees pour cette etude car des differents types de cellules testes (hepatomes ascitiques, reticulocytes et erythrocytes), ce sont elles qui presentent la meilleure permeabilite a la canavanine. Cette substance penetre dans les bacteries par un mecanisme de transport actif. La canavanine inhibe de facon irreversible la division cellulaire des bacteries. Cette perte de viabilite est liee a l'incorporation de la canavanine a la place de l'arginine dans les proteines. La canavanine stimule la synthese de l'ARN dans les souches de bacteries RC{sup str} arg{sup -}, carencees en arginine; mais elle inhibe l'incorporation de l'uracile, l'UMP et d'UDP dans les souches RC{sup rel} arg{sup -}. En presence de canavanine, la replication de l'ADN suit le meme type de cinetique que dans les bacteries carencees en acide amine indispensable. L'ADN des bacteries incubees en presence de canavanine presente des alterations qui resultent de coupures de la molecule d'ADN. Il s'ensuit la formation de fragments d'ADN qui ne sedimentent pas a 10 000 xg avec les membranes cellulaires, ni a 150 000 xg en gradient de sucrose alcalin. L'action lethale de la canavanine doit etre le resultat de cette alteration de l'ADN. (auteur)}
place = {France}
year = {1972}
month = {Jan}
}