Abstract
Highlights: Black-Right-Pointing-Pointer Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription. Black-Right-Pointing-Pointer Adipose lipin-1 expression is reduced in obesity. Black-Right-Pointing-Pointer Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression. Black-Right-Pointing-Pointer Lipin-1 is involved in adipose inflammation. -- Abstract: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-{kappa}B activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.
Takahashi, Nobuhiko;
[1]
Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)];
Yoshizaki, Takayuki;
[2]
Hiranaka, Natsumi;
Suzuki, Takeshi;
[1]
Yui, Tomoo;
Akanuma, Masayasu;
Oka, Kazuya;
[3]
Kanazawa, Kaoru;
[4]
Yoshida, Mika;
Naito, Sumiyoshi;
[5]
Fujiya, Mikihiro;
Kohgo, Yutaka;
[6]
Ieko, Masahiro
[1]
- Department of Internal Medicine, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
- Innovation Center, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065 (Japan)
- Department of Fixed Prosthodontics and Oral Implantology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
- Department of Dental Anesthesiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
- Department of Clinical Laboratory, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Toubetsu, Hokkaido 061-0023 (Japan)
- Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)
Citation Formats
Takahashi, Nobuhiko, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)], Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro.
Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes.
United States: N. p.,
2011.
Web.
doi:10.1016/J.BBRC.2011.10.060.
Takahashi, Nobuhiko, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)], Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, & Ieko, Masahiro.
Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes.
United States.
https://doi.org/10.1016/J.BBRC.2011.10.060
Takahashi, Nobuhiko, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)], Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro.
2011.
"Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes."
United States.
https://doi.org/10.1016/J.BBRC.2011.10.060.
@misc{etde_22207568,
title = {Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes}
author = {Takahashi, Nobuhiko, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)], Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro}
abstractNote = {Highlights: Black-Right-Pointing-Pointer Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription. Black-Right-Pointing-Pointer Adipose lipin-1 expression is reduced in obesity. Black-Right-Pointing-Pointer Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression. Black-Right-Pointing-Pointer Lipin-1 is involved in adipose inflammation. -- Abstract: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-{kappa}B activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.}
doi = {10.1016/J.BBRC.2011.10.060}
journal = []
issue = {1}
volume = {415}
journal type = {AC}
place = {United States}
year = {2011}
month = {Nov}
}
title = {Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes}
author = {Takahashi, Nobuhiko, Division of Gastroenterology and Hematology/Oncology, Department of Medicine, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa, Hokkaido 078-8510 (Japan)], Yoshizaki, Takayuki, Hiranaka, Natsumi, Suzuki, Takeshi, Yui, Tomoo, Akanuma, Masayasu, Oka, Kazuya, Kanazawa, Kaoru, Yoshida, Mika, Naito, Sumiyoshi, Fujiya, Mikihiro, Kohgo, Yutaka, and Ieko, Masahiro}
abstractNote = {Highlights: Black-Right-Pointing-Pointer Lipin-1 affects lipid metabolism, adipocyte differentiation, and transcription. Black-Right-Pointing-Pointer Adipose lipin-1 expression is reduced in obesity. Black-Right-Pointing-Pointer Lipin-1 depletion using siRNA in 3T3-L1 adipocytes increased MCP-1 expression. Black-Right-Pointing-Pointer Lipin-1 is involved in adipose inflammation. -- Abstract: Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-{kappa}B activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.}
doi = {10.1016/J.BBRC.2011.10.060}
journal = []
issue = {1}
volume = {415}
journal type = {AC}
place = {United States}
year = {2011}
month = {Nov}
}