Abstract
Highlights: {yields} OxLDL-induced responses in human gingival epithelial cells were studied. {yields} OxLDL enhanced the production of IL-8, IL-1{beta} and PGE{sub 2} in Ca9-22 cells. {yields} An NF-{kappa}B inhibitor suppressed the expression of COX-2 and mPGES1 induced by oxLDL. {yields} Unlike the case in macrophages, oxLDL did not increase the CD36 level. -- Abstract: Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E{sub 2} (PGE{sub 2}) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE{sub 2}-producing enzymes, cyclooxygenase-2 and microsomal PGE{sub 2} synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-{kappa}B) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-{kappa}B pathway. These data suggest
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Nagahama, Yu;
[1]
Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)];
Obama, Takashi;
[2]
Usui, Michihiko;
[1]
Kanazawa, Yukari;
[2]
Iwamoto, Sanju;
[3]
Suzuki, Kazushige;
[1]
Miyazaki, Akira;
[3]
Yamaguchi, Tomohiro;
[2]
Yamamoto, Matsuo;
[1]
Itabe, Hiroyuki
[2]
- Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo (Japan)
- Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)
- Department of Biochemistry, Showa University School of Medicine, Tokyo (Japan)
Citation Formats
Nagahama, Yu, Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)], Obama, Takashi, Usui, Michihiko, Kanazawa, Yukari, Iwamoto, Sanju, Suzuki, Kazushige, Miyazaki, Akira, Yamaguchi, Tomohiro, Yamamoto, Matsuo, and Itabe, Hiroyuki.
Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells.
United States: N. p.,
2011.
Web.
doi:10.1016/J.BBRC.2011.09.002.
Nagahama, Yu, Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)], Obama, Takashi, Usui, Michihiko, Kanazawa, Yukari, Iwamoto, Sanju, Suzuki, Kazushige, Miyazaki, Akira, Yamaguchi, Tomohiro, Yamamoto, Matsuo, & Itabe, Hiroyuki.
Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells.
United States.
https://doi.org/10.1016/J.BBRC.2011.09.002
Nagahama, Yu, Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)], Obama, Takashi, Usui, Michihiko, Kanazawa, Yukari, Iwamoto, Sanju, Suzuki, Kazushige, Miyazaki, Akira, Yamaguchi, Tomohiro, Yamamoto, Matsuo, and Itabe, Hiroyuki.
2011.
"Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells."
United States.
https://doi.org/10.1016/J.BBRC.2011.09.002.
@misc{etde_22207514,
title = {Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells}
author = {Nagahama, Yu, Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)], Obama, Takashi, Usui, Michihiko, Kanazawa, Yukari, Iwamoto, Sanju, Suzuki, Kazushige, Miyazaki, Akira, Yamaguchi, Tomohiro, Yamamoto, Matsuo, and Itabe, Hiroyuki}
abstractNote = {Highlights: {yields} OxLDL-induced responses in human gingival epithelial cells were studied. {yields} OxLDL enhanced the production of IL-8, IL-1{beta} and PGE{sub 2} in Ca9-22 cells. {yields} An NF-{kappa}B inhibitor suppressed the expression of COX-2 and mPGES1 induced by oxLDL. {yields} Unlike the case in macrophages, oxLDL did not increase the CD36 level. -- Abstract: Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E{sub 2} (PGE{sub 2}) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE{sub 2}-producing enzymes, cyclooxygenase-2 and microsomal PGE{sub 2} synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-{kappa}B) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-{kappa}B pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.}
doi = {10.1016/J.BBRC.2011.09.002}
journal = []
issue = {4}
volume = {413}
journal type = {AC}
place = {United States}
year = {2011}
month = {Oct}
}
title = {Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells}
author = {Nagahama, Yu, Department of Biological Chemistry, Showa University School of Pharmacy, Tokyo (Japan)], Obama, Takashi, Usui, Michihiko, Kanazawa, Yukari, Iwamoto, Sanju, Suzuki, Kazushige, Miyazaki, Akira, Yamaguchi, Tomohiro, Yamamoto, Matsuo, and Itabe, Hiroyuki}
abstractNote = {Highlights: {yields} OxLDL-induced responses in human gingival epithelial cells were studied. {yields} OxLDL enhanced the production of IL-8, IL-1{beta} and PGE{sub 2} in Ca9-22 cells. {yields} An NF-{kappa}B inhibitor suppressed the expression of COX-2 and mPGES1 induced by oxLDL. {yields} Unlike the case in macrophages, oxLDL did not increase the CD36 level. -- Abstract: Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E{sub 2} (PGE{sub 2}) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE{sub 2}-producing enzymes, cyclooxygenase-2 and microsomal PGE{sub 2} synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-{kappa}B) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-{kappa}B pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.}
doi = {10.1016/J.BBRC.2011.09.002}
journal = []
issue = {4}
volume = {413}
journal type = {AC}
place = {United States}
year = {2011}
month = {Oct}
}