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Labelled T{sub 3}, T{sub 4} and TBP for In Vitro Testing of Thyroid Function in Man and Animals

Abstract

Iodothyronines are bound to determined electrophoretic fractions of serum proteins - TBP (TBG, TBA, TBPA). Radioiodine labelled T{sub 3} and T{sub 4} complex the free TBP fractions until saturation. The excess of added in vitro thyronines is then absorbed by the RBC. The changes described can be detected and quantitatively determined by radioisotope tests: radioelectrophoresis - T{sub 3/4} BP test, and RBC - {sup 125}I T{sub 3} test (Hamolsky test). The in vitro tests of the thyroid function can be clinically reliable if the protein fractions are normal, and they may be altered without thyroid pathology if the TBG fractions are abnormal. The electrophoretic fractions vary quantitatively and qualitatively in animals of different classes and orders. We chose these features to study the correlation between iodothyronines, plasma proteins and the above-mentioned thyroid tests. Twenty-two animal species (arranged according to the increasing percentage of the Hamolsky test) were examined: goat (8.6%, cow, lamb, calf, man, camel, goose, hamster, rat, turkey, marmot, duck, horse, donkey, hen, dog, pigeon, rabbit, guinea-pig, mouse, fish and frog (91.5%). The following additional parameters were examined: PBI, serum quantitative electrophoresis, T{sub 3} BP studies. All the tests were performed under identical technical conditions. Results and conclusions: (1)  More>>
Authors:
Czerniak, P.; Boruchowski, Sabina; Shomron, I. [1] 
  1. Dept. of Radiotherapy and Isotopes, Tel-Hashomer Hospital, Tel-Aviv University Medical School, Faculty of Continuing Medical Education, Tel-Aviv (Israel)
Publication Date:
Feb 15, 1970
Product Type:
Conference
Report Number:
IAEA-SM-124/47
Resource Relation:
Conference: Symposium on In Vitro Procedures with Radioisotopes in Clinical Medicine and Research, Vienna (Austria), 8-12 Sep 1969; Other Information: 20 refs., 5 tabs., 4 figs.; Related Information: In: In Vitro Procedures with Radioisotopes in Medicine. Proceedings of the Symposium on In Vitro Procedures with Radioistopes in Clinical Medicine and Research| 736 p.
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DIAGNOSTIC TECHNIQUES; DIAGNOSTIC USES; DIIODOTHYRONINE; DOMESTIC ANIMALS; NUCLEAR MEDICINE; RADIOPHARMACEUTICALS; THYRONINE
OSTI ID:
22205116
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); World Health Organization, Geneva (Switzerland)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M4197026028
Submitting Site:
INIS
Size:
page(s) 273-286
Announcement Date:
Mar 14, 2014

Citation Formats

Czerniak, P., Boruchowski, Sabina, and Shomron, I. Labelled T{sub 3}, T{sub 4} and TBP for In Vitro Testing of Thyroid Function in Man and Animals. IAEA: N. p., 1970. Web.
Czerniak, P., Boruchowski, Sabina, & Shomron, I. Labelled T{sub 3}, T{sub 4} and TBP for In Vitro Testing of Thyroid Function in Man and Animals. IAEA.
Czerniak, P., Boruchowski, Sabina, and Shomron, I. 1970. "Labelled T{sub 3}, T{sub 4} and TBP for In Vitro Testing of Thyroid Function in Man and Animals." IAEA.
@misc{etde_22205116,
title = {Labelled T{sub 3}, T{sub 4} and TBP for In Vitro Testing of Thyroid Function in Man and Animals}
author = {Czerniak, P., Boruchowski, Sabina, and Shomron, I.}
abstractNote = {Iodothyronines are bound to determined electrophoretic fractions of serum proteins - TBP (TBG, TBA, TBPA). Radioiodine labelled T{sub 3} and T{sub 4} complex the free TBP fractions until saturation. The excess of added in vitro thyronines is then absorbed by the RBC. The changes described can be detected and quantitatively determined by radioisotope tests: radioelectrophoresis - T{sub 3/4} BP test, and RBC - {sup 125}I T{sub 3} test (Hamolsky test). The in vitro tests of the thyroid function can be clinically reliable if the protein fractions are normal, and they may be altered without thyroid pathology if the TBG fractions are abnormal. The electrophoretic fractions vary quantitatively and qualitatively in animals of different classes and orders. We chose these features to study the correlation between iodothyronines, plasma proteins and the above-mentioned thyroid tests. Twenty-two animal species (arranged according to the increasing percentage of the Hamolsky test) were examined: goat (8.6%, cow, lamb, calf, man, camel, goose, hamster, rat, turkey, marmot, duck, horse, donkey, hen, dog, pigeon, rabbit, guinea-pig, mouse, fish and frog (91.5%). The following additional parameters were examined: PBI, serum quantitative electrophoresis, T{sub 3} BP studies. All the tests were performed under identical technical conditions. Results and conclusions: (1) The Hamolsky test in the examined animals ranges from 9% to 92%. It is highest in the poicolothermics, in which much prealbumin and few glubulins are found on electrophoresis. The T{sub 3} BP is low, and about a half of the added {sup 125}I T{sub 3} remains unbound. (2) T{sub 3} and T{sub 4} are complexed with T{sub 3} BP and T{sub 4} BP fractions, which correspond to prealbumin, albumin, alpha 1-2, beta and exceptionally gamma globulin. The fractions are variable but characteristic for each animal species. T{sub 3} BP does not correlate exactly with T{sub 4} BP, and seems to be distributed over more fractions. Diminution of T{sub 3} BP in the serum of a given animal results in an elevated Hamolsky test (frog, fish, pigeon, guinea-pig). (3) There is no correspondence between the RBC - I- T{sub 3} test and the PBI of the animals examined (e.g. in fish: PBI = 1.1{gamma}% and Hamolsky test = 68%; in rabbit: PBI = 1.1 {gamma}% too and Hamolsky = 47%). The ascending curve of the Hamolsky tests crosses the descending curve of PBI (passing from goat to frog) in the rat-position. (4) The in vitro tests examined remain specific but unchanged for each animal species, if the serum and the technique used remain unchanged. If the electrophoretic fractions change, the results of the in vitro tests change too, becoming similar to another species. (5) The human serum composition is generally stable and the in vitro tests are reliable in 70-80% of these cases. In cases of discrepancy and for a complete study of the RBC-I T{sub 3} test it is advisable to add serum electrophoresis and T{sub 3} BP determination. (author)}
place = {IAEA}
year = {1970}
month = {Feb}
}