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The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice

Abstract

Research highlights: {yields} Aging is associated with muscle atrophy and loss of muscle mass, known as the sarcopenia of aging. {yields} We demonstrate that age-related muscle atrophy is associated with marked changes in miRNA expression in muscle. {yields} Treating aged mice with the adipokine leptin significantly increased muscle mass and the expression of miRNAs involved in muscle repair. {yields} Recombinant leptin therapy may therefore be a novel approach for treating age-related muscle atrophy. -- Abstract: Age-associated loss of muscle mass, or sarcopenia, contributes directly to frailty and an increased risk of falls and fractures among the elderly. Aged mice and elderly adults both show decreased muscle mass as well as relatively low levels of the fat-derived hormone leptin. Here we demonstrate that loss of muscle mass and myofiber size with aging in mice is associated with significant changes in the expression of specific miRNAs. Aging altered the expression of 57 miRNAs in mouse skeletal muscle, and many of these miRNAs are now reported to be associated specifically with age-related muscle atrophy. These include miR-221, previously identified in studies of myogenesis and muscle development as playing a role in the proliferation and terminal differentiation of myogenic precursors. We also treated aged  More>>
Authors:
Hamrick, Mark W., E-mail: mhamrick@mail.mcg.edu [Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States); Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)]; Herberg, Samuel; Arounleut, Phonepasong; [1]  Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)]; He, Hong-Zhi; [2]  Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)]; Shiver, Austin; [1]  Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)]; Qi, Rui-Qun; [2]  Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)]; Zhou, Li; [2]  Department of Dermatology, Henry Ford Health System, Detroit, MI (United States); Department of Internal Medicine, Henry Ford Health System, Detroit, MI (United States)]; Isales, Carlos M.; [1]  Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)]; others, and
  1. Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)
  2. Henry Ford Immunology Program, Henry Ford Health System, Detroit, MI (United States)
Publication Date:
Sep 24, 2010
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 400; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; AGING; ELDERLY PEOPLE; FATS; FIBERS; HAZARDS; MASS; MICE; MUSCLES; NUTRIENTS
OSTI ID:
22202780
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; CODEN: BBRCA9; Other: PII: S0006-291X(10)01589-5; TRN: US10R2206023691
Availability:
Available from http://dx.doi.org/10.1016/j.bbrc.2010.08.079
Submitting Site:
USN
Size:
page(s) 379-383
Announcement Date:
Mar 06, 2014

Citation Formats

Hamrick, Mark W., E-mail: mhamrick@mail.mcg.edu [Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States), Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Herberg, Samuel, Arounleut, Phonepasong, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], He, Hong-Zhi, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Shiver, Austin, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Qi, Rui-Qun, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Zhou, Li, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States), Department of Internal Medicine, Henry Ford Health System, Detroit, MI (United States)], Isales, Carlos M., Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], and others, and. The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.08.079.
Hamrick, Mark W., E-mail: mhamrick@mail.mcg.edu [Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States), Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Herberg, Samuel, Arounleut, Phonepasong, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], He, Hong-Zhi, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Shiver, Austin, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Qi, Rui-Qun, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Zhou, Li, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States), Department of Internal Medicine, Henry Ford Health System, Detroit, MI (United States)], Isales, Carlos M., Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], & others, and. The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice. United States. doi:10.1016/J.BBRC.2010.08.079.
Hamrick, Mark W., E-mail: mhamrick@mail.mcg.edu [Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States), Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Herberg, Samuel, Arounleut, Phonepasong, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], He, Hong-Zhi, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Shiver, Austin, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Qi, Rui-Qun, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Zhou, Li, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States), Department of Internal Medicine, Henry Ford Health System, Detroit, MI (United States)], Isales, Carlos M., Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], and others, and. 2010. "The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice." United States. doi:10.1016/J.BBRC.2010.08.079. https://www.osti.gov/servlets/purl/10.1016/J.BBRC.2010.08.079.
@misc{etde_22202780,
title = {The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice}
author = {Hamrick, Mark W., E-mail: mhamrick@mail.mcg.edu [Department of Cellular Biology and Anatomy, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States), Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Herberg, Samuel, Arounleut, Phonepasong, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], He, Hong-Zhi, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Shiver, Austin, Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], Qi, Rui-Qun, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States)], Zhou, Li, Department of Dermatology, Henry Ford Health System, Detroit, MI (United States), Department of Internal Medicine, Henry Ford Health System, Detroit, MI (United States)], Isales, Carlos M., Department of Orthopaedic Surgery, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA (United States)], and others, and}
abstractNote = {Research highlights: {yields} Aging is associated with muscle atrophy and loss of muscle mass, known as the sarcopenia of aging. {yields} We demonstrate that age-related muscle atrophy is associated with marked changes in miRNA expression in muscle. {yields} Treating aged mice with the adipokine leptin significantly increased muscle mass and the expression of miRNAs involved in muscle repair. {yields} Recombinant leptin therapy may therefore be a novel approach for treating age-related muscle atrophy. -- Abstract: Age-associated loss of muscle mass, or sarcopenia, contributes directly to frailty and an increased risk of falls and fractures among the elderly. Aged mice and elderly adults both show decreased muscle mass as well as relatively low levels of the fat-derived hormone leptin. Here we demonstrate that loss of muscle mass and myofiber size with aging in mice is associated with significant changes in the expression of specific miRNAs. Aging altered the expression of 57 miRNAs in mouse skeletal muscle, and many of these miRNAs are now reported to be associated specifically with age-related muscle atrophy. These include miR-221, previously identified in studies of myogenesis and muscle development as playing a role in the proliferation and terminal differentiation of myogenic precursors. We also treated aged mice with recombinant leptin, to determine whether leptin therapy could improve muscle mass and alter the miRNA expression profile of aging skeletal muscle. Leptin treatment significantly increased hindlimb muscle mass and extensor digitorum longus fiber size in aged mice. Furthermore, the expression of 37 miRNAs was altered in muscles of leptin-treated mice. In particular, leptin treatment increased the expression of miR-31 and miR-223, miRNAs known to be elevated during muscle regeneration and repair. These findings suggest that aging in skeletal muscle is associated with marked changes in the expression of specific miRNAs, and that nutrient-related hormones such as leptin may be able to reverse muscle atrophy and alter the expression of atrophy-related miRNAs in aging skeletal muscle.}
doi = {10.1016/J.BBRC.2010.08.079}
journal = {Biochemical and Biophysical Research Communications}
issue = {3}
volume = {400}
journal type = {AC}
place = {United States}
year = {2010}
month = {Sep}
}