Abstract
Common bean (Phaseolus vulgaris L.) represents the main source of protein for the Brazilian and other Latin-American populations. Unlike soybean, which is very efficient in fixing atmospheric N{sub 2} symbiotically, common bean does not dispense with the need for N fertilizer application, as the biologically fixed N (BNF) seems incapable to supplement the total N required by the crop. A experiment under controlled conditions was conducted in Piracicaba, Brazil, to assess N{sub 2} fixation of 25 genotypes of common bean (Phaseolus vulgaris L.). BNF was measured by {sup 15}N isotope dilution using a non-N{sub 2} fixing bean genotype as a reference crop. The common bean genotypes were grown in low (2.2 mg N kg{sup -1} soil) or high N content soil (200 mg N kg{sup -1} soil), through N fertilizer application, as urea-{sup 15}N (31.20 and 1.4 atom % {sup 15}N, respectively). The bean seeds were inoculated with Rhizobium tropici CIAT 899 strain and the plants were harvested at grain maturity stage. The contribution of BNF was on average 75% of total plant N content, and there were differences in N fixing capacity among the bean genotypes. The most efficient genotypes were Horizonte, Roxo 90, Grafite, Apore and Vereda, when
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Franzini, V. I.;
Mendes, F. L.;
[1]
Muraoka, T.;
Trevisam, A. R.;
[2]
Adu-Gyamfi, J. J.
[3]
- Brazilian Agricultural Research Corporation, EMBRAPA-Amazonia Oriental, Belem, PA (Brazil)
- Center for Nuclear Energy in Agriculture, University of Sao Paulo, Piracicaba, SP (Brazil)
- Soil and Water Management and Crop Nutrition Laboratory, International Atomic Energy Agency, Seibersdorf (Austria)
Citation Formats
Franzini, V. I., Mendes, F. L., Muraoka, T., Trevisam, A. R., and Adu-Gyamfi, J. J.
Biological Nitrogen Fixation Efficiency in Brazilian Common Bean Genotypes as Measured by {sup 15}N Methodology.
IAEA: N. p.,
2013.
Web.
Franzini, V. I., Mendes, F. L., Muraoka, T., Trevisam, A. R., & Adu-Gyamfi, J. J.
Biological Nitrogen Fixation Efficiency in Brazilian Common Bean Genotypes as Measured by {sup 15}N Methodology.
IAEA.
Franzini, V. I., Mendes, F. L., Muraoka, T., Trevisam, A. R., and Adu-Gyamfi, J. J.
2013.
"Biological Nitrogen Fixation Efficiency in Brazilian Common Bean Genotypes as Measured by {sup 15}N Methodology."
IAEA.
@misc{etde_22192693,
title = {Biological Nitrogen Fixation Efficiency in Brazilian Common Bean Genotypes as Measured by {sup 15}N Methodology}
author = {Franzini, V. I., Mendes, F. L., Muraoka, T., Trevisam, A. R., and Adu-Gyamfi, J. J.}
abstractNote = {Common bean (Phaseolus vulgaris L.) represents the main source of protein for the Brazilian and other Latin-American populations. Unlike soybean, which is very efficient in fixing atmospheric N{sub 2} symbiotically, common bean does not dispense with the need for N fertilizer application, as the biologically fixed N (BNF) seems incapable to supplement the total N required by the crop. A experiment under controlled conditions was conducted in Piracicaba, Brazil, to assess N{sub 2} fixation of 25 genotypes of common bean (Phaseolus vulgaris L.). BNF was measured by {sup 15}N isotope dilution using a non-N{sub 2} fixing bean genotype as a reference crop. The common bean genotypes were grown in low (2.2 mg N kg{sup -1} soil) or high N content soil (200 mg N kg{sup -1} soil), through N fertilizer application, as urea-{sup 15}N (31.20 and 1.4 atom % {sup 15}N, respectively). The bean seeds were inoculated with Rhizobium tropici CIAT 899 strain and the plants were harvested at grain maturity stage. The contribution of BNF was on average 75% of total plant N content, and there were differences in N fixing capacity among the bean genotypes. The most efficient genotypes were Horizonte, Roxo 90, Grafite, Apore and Vereda, when grown in high N soil. None of the genotypes grown in low N soil was efficient in producing grains compared to those grown in high N soil, and therefore the BNF was not able to supply the total N demand of the bean crop. (author)}
place = {IAEA}
year = {2013}
month = {Nov}
}
title = {Biological Nitrogen Fixation Efficiency in Brazilian Common Bean Genotypes as Measured by {sup 15}N Methodology}
author = {Franzini, V. I., Mendes, F. L., Muraoka, T., Trevisam, A. R., and Adu-Gyamfi, J. J.}
abstractNote = {Common bean (Phaseolus vulgaris L.) represents the main source of protein for the Brazilian and other Latin-American populations. Unlike soybean, which is very efficient in fixing atmospheric N{sub 2} symbiotically, common bean does not dispense with the need for N fertilizer application, as the biologically fixed N (BNF) seems incapable to supplement the total N required by the crop. A experiment under controlled conditions was conducted in Piracicaba, Brazil, to assess N{sub 2} fixation of 25 genotypes of common bean (Phaseolus vulgaris L.). BNF was measured by {sup 15}N isotope dilution using a non-N{sub 2} fixing bean genotype as a reference crop. The common bean genotypes were grown in low (2.2 mg N kg{sup -1} soil) or high N content soil (200 mg N kg{sup -1} soil), through N fertilizer application, as urea-{sup 15}N (31.20 and 1.4 atom % {sup 15}N, respectively). The bean seeds were inoculated with Rhizobium tropici CIAT 899 strain and the plants were harvested at grain maturity stage. The contribution of BNF was on average 75% of total plant N content, and there were differences in N fixing capacity among the bean genotypes. The most efficient genotypes were Horizonte, Roxo 90, Grafite, Apore and Vereda, when grown in high N soil. None of the genotypes grown in low N soil was efficient in producing grains compared to those grown in high N soil, and therefore the BNF was not able to supply the total N demand of the bean crop. (author)}
place = {IAEA}
year = {2013}
month = {Nov}
}