Abstract
Cowpea (Vigna unguiculata L. Walp) is an important food legume. In Africa, it is mostly cultivated under such environmental constraints as drought and pest, and nutrient deficiency. In particular low soil phosphorus strongly limits crop production for the poor farmers with limited access to P fertilizers. Therefore breeding cowpea for the tolerance to P deficiency is considered as an alternative to increase the productivity of traditional cowpea-cereal cropping systems in soils with low P availability. This paper reports cowpea genotypic-variation in P use efficiency for symbiotic nitrogen fixation as a contribution to select tolerant cowpea lines under P deficiency. Eighty cowpea cultivars inoculated with the reference strain of Bradyrhizobium sp. Vigna CB756 were pre-screened as a single replicate under hydroaeroponic culture for 6 weeks under P deficiency versus P sufficiency, namely 15 vs 30 {mu}mol plant{sup -1} week{sup -1}. Large variability in nodule number per plant, and in shoot growth as a function of nodule mass, was observed among the diversity of cowpea lines. From this pre-screening experiment, the 40 cowpea lines showing the highest SNF-potential, i.e. high nodulation linked with high N{sub 2}-dependent growth under P sufficiency, and the most contrasting tolerance to P deficiency, i.e. highest vs lowest
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Andriamananjara, A.;
[1]
Abdou, M. Malam;
[2]
Pernot, C.;
Drevon, J. J.
[3]
- LRI-SRA, Laboratoire des Radio-isotopes, Universite d'Antananarivo, Antananarivo (Madagascar)
- Laboratoire Banques de genes CERRA / KOLLO, Institut National de Recherche Agronomique du Niger (INRAN), Niamey (Niger)
- Institut National de la Recherche Agronomique, UMR Eco and Sols, Montpellier (France)
Citation Formats
Andriamananjara, A., Abdou, M. Malam, Pernot, C., and Drevon, J. J.
Genotypic Variation in Phosphorus Use Efficiency for Symbiotic Nitrogen Fixation in Cowpea (Vigna Unguiculata).
IAEA: N. p.,
2013.
Web.
Andriamananjara, A., Abdou, M. Malam, Pernot, C., & Drevon, J. J.
Genotypic Variation in Phosphorus Use Efficiency for Symbiotic Nitrogen Fixation in Cowpea (Vigna Unguiculata).
IAEA.
Andriamananjara, A., Abdou, M. Malam, Pernot, C., and Drevon, J. J.
2013.
"Genotypic Variation in Phosphorus Use Efficiency for Symbiotic Nitrogen Fixation in Cowpea (Vigna Unguiculata)."
IAEA.
@misc{etde_22192685,
title = {Genotypic Variation in Phosphorus Use Efficiency for Symbiotic Nitrogen Fixation in Cowpea (Vigna Unguiculata)}
author = {Andriamananjara, A., Abdou, M. Malam, Pernot, C., and Drevon, J. J.}
abstractNote = {Cowpea (Vigna unguiculata L. Walp) is an important food legume. In Africa, it is mostly cultivated under such environmental constraints as drought and pest, and nutrient deficiency. In particular low soil phosphorus strongly limits crop production for the poor farmers with limited access to P fertilizers. Therefore breeding cowpea for the tolerance to P deficiency is considered as an alternative to increase the productivity of traditional cowpea-cereal cropping systems in soils with low P availability. This paper reports cowpea genotypic-variation in P use efficiency for symbiotic nitrogen fixation as a contribution to select tolerant cowpea lines under P deficiency. Eighty cowpea cultivars inoculated with the reference strain of Bradyrhizobium sp. Vigna CB756 were pre-screened as a single replicate under hydroaeroponic culture for 6 weeks under P deficiency versus P sufficiency, namely 15 vs 30 {mu}mol plant{sup -1} week{sup -1}. Large variability in nodule number per plant, and in shoot growth as a function of nodule mass, was observed among the diversity of cowpea lines. From this pre-screening experiment, the 40 cowpea lines showing the highest SNF-potential, i.e. high nodulation linked with high N{sub 2}-dependent growth under P sufficiency, and the most contrasting tolerance to P deficiency, i.e. highest vs lowest N{sub 2}-dependent growth under P deficiency, were grown again in glasshouse hydroaeroponics with 6 replicates. As an illustration of the most contrasting lines, the nodulation was decreased under P deficiency by less than 20% for IT82E-18 whereas by more than 80% for IT95K-1105-5 or SUVITA 2. The variations in nodulation were correlated with variations in growth with mean value of additional growth per unit increase in nodule biomass of 23 g shoot DW g-1 nodule DW under P sufficiency, showing 3 lines showing exceptionally high potential for symbiotic nitrogen fixation, versus 28 g shoot DW g{sup -1} nodule DW showing large variation among lines. Most of the tolerance to P deficiency was due to increase in the nodule function rather than conservation of nodule mass. In conclusion, the screening in glasshouse hydroaeroponics made it possible to sort cowpea lines in SNF potential to fix N{sub 2}, and in tolerance to P deficiency. (author)}
place = {IAEA}
year = {2013}
month = {Nov}
}
title = {Genotypic Variation in Phosphorus Use Efficiency for Symbiotic Nitrogen Fixation in Cowpea (Vigna Unguiculata)}
author = {Andriamananjara, A., Abdou, M. Malam, Pernot, C., and Drevon, J. J.}
abstractNote = {Cowpea (Vigna unguiculata L. Walp) is an important food legume. In Africa, it is mostly cultivated under such environmental constraints as drought and pest, and nutrient deficiency. In particular low soil phosphorus strongly limits crop production for the poor farmers with limited access to P fertilizers. Therefore breeding cowpea for the tolerance to P deficiency is considered as an alternative to increase the productivity of traditional cowpea-cereal cropping systems in soils with low P availability. This paper reports cowpea genotypic-variation in P use efficiency for symbiotic nitrogen fixation as a contribution to select tolerant cowpea lines under P deficiency. Eighty cowpea cultivars inoculated with the reference strain of Bradyrhizobium sp. Vigna CB756 were pre-screened as a single replicate under hydroaeroponic culture for 6 weeks under P deficiency versus P sufficiency, namely 15 vs 30 {mu}mol plant{sup -1} week{sup -1}. Large variability in nodule number per plant, and in shoot growth as a function of nodule mass, was observed among the diversity of cowpea lines. From this pre-screening experiment, the 40 cowpea lines showing the highest SNF-potential, i.e. high nodulation linked with high N{sub 2}-dependent growth under P sufficiency, and the most contrasting tolerance to P deficiency, i.e. highest vs lowest N{sub 2}-dependent growth under P deficiency, were grown again in glasshouse hydroaeroponics with 6 replicates. As an illustration of the most contrasting lines, the nodulation was decreased under P deficiency by less than 20% for IT82E-18 whereas by more than 80% for IT95K-1105-5 or SUVITA 2. The variations in nodulation were correlated with variations in growth with mean value of additional growth per unit increase in nodule biomass of 23 g shoot DW g-1 nodule DW under P sufficiency, showing 3 lines showing exceptionally high potential for symbiotic nitrogen fixation, versus 28 g shoot DW g{sup -1} nodule DW showing large variation among lines. Most of the tolerance to P deficiency was due to increase in the nodule function rather than conservation of nodule mass. In conclusion, the screening in glasshouse hydroaeroponics made it possible to sort cowpea lines in SNF potential to fix N{sub 2}, and in tolerance to P deficiency. (author)}
place = {IAEA}
year = {2013}
month = {Nov}
}