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Bone-Marrow Storage and Transplantation

Abstract

The authors present some results from their experiments on bone-marrow storage and transplantation. The main problems with preservation of stored bone marrow are the duration, temperature, adjuvant substances and the significance of viability tests during the conservation processes. The results showed that: Bullet Storage of bone marrow at +4eC produces a progressive decrease in its restoring capacity versus storage time. Bullet While bone marrow stored for 24 h is able to restore 100% of dogs lethally irradiated with 600 rad, after 10 days of storage only 20% of the animals can be restored. Bullet No correlation exists between the actual survival of dogs and that calculated by dye exclusion tests, which indicate a rather high (70%) viability, even after 10 days bone-marrow storage at +4 Degree-Sign C. Bullet DNA degradation (depolymerization) measurements of the bone marrow may be used as a supplementary test for checking the viability or restoration potency of bone-marrow cells after storage. Bullet In the freezing process, the optimum contact time between glycerol and the bone-marrow cells is 15 min. Results of experiments regarding certain bone-marrow transplantation problems showed that: Bullet The best time to administer bone marrow is between 24 and 48 h after irradiation. Bullet  More>>
Authors:
Costachel, O.; Corneci, I.; Andrian, T.; Kitzulescu, I.; Popescu, N.; Pascu, D.; Buzi, E.; Voiculetz, N. [1] 
  1. Oncological Institute, Bucharest (Romania)
Publication Date:
Jul 15, 1969
Product Type:
Conference
Resource Relation:
Conference: Panel on Current Problems of Bone-Marrow Cell Transplantation with Special Emphasis on Conservation and Culture, Moscow (Russian Federation), 22-26 Jul 1968; Other Information: 68 refs., 10 tabs., 9 figs.; Related Information: In: Bone-Marrow Conservation, Culture and Transplantation. Proceedings of a Panel on Current Problems of Bone-Marrow Cell Transplantation with Special Emphasis on Conservation and Culture| 211 p.
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD; BONE MARROW; BONE MARROW CELLS; DEPOLYMERIZATION; DNA; DOGS; FREEZING; GLYCEROL; GRAFTS; LIVER; MICE; RADIOPRESERVATION; RADIOSTERILIZATION; RATS; SPLEEN; TRANSFUSIONS
OSTI ID:
22192480
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Contract Number:
Contract IAEA 226/RB
Other Identifying Numbers:
TRN: XA14M0717016969
Submitting Site:
INIS
Size:
page(s) 143-161
Announcement Date:
Feb 20, 2014

Citation Formats

Costachel, O., Corneci, I., Andrian, T., Kitzulescu, I., Popescu, N., Pascu, D., Buzi, E., and Voiculetz, N. Bone-Marrow Storage and Transplantation. IAEA: N. p., 1969. Web.
Costachel, O., Corneci, I., Andrian, T., Kitzulescu, I., Popescu, N., Pascu, D., Buzi, E., & Voiculetz, N. Bone-Marrow Storage and Transplantation. IAEA.
Costachel, O., Corneci, I., Andrian, T., Kitzulescu, I., Popescu, N., Pascu, D., Buzi, E., and Voiculetz, N. 1969. "Bone-Marrow Storage and Transplantation." IAEA.
@misc{etde_22192480,
title = {Bone-Marrow Storage and Transplantation}
author = {Costachel, O., Corneci, I., Andrian, T., Kitzulescu, I., Popescu, N., Pascu, D., Buzi, E., and Voiculetz, N.}
abstractNote = {The authors present some results from their experiments on bone-marrow storage and transplantation. The main problems with preservation of stored bone marrow are the duration, temperature, adjuvant substances and the significance of viability tests during the conservation processes. The results showed that: Bullet Storage of bone marrow at +4eC produces a progressive decrease in its restoring capacity versus storage time. Bullet While bone marrow stored for 24 h is able to restore 100% of dogs lethally irradiated with 600 rad, after 10 days of storage only 20% of the animals can be restored. Bullet No correlation exists between the actual survival of dogs and that calculated by dye exclusion tests, which indicate a rather high (70%) viability, even after 10 days bone-marrow storage at +4 Degree-Sign C. Bullet DNA degradation (depolymerization) measurements of the bone marrow may be used as a supplementary test for checking the viability or restoration potency of bone-marrow cells after storage. Bullet In the freezing process, the optimum contact time between glycerol and the bone-marrow cells is 15 min. Results of experiments regarding certain bone-marrow transplantation problems showed that: Bullet The best time to administer bone marrow is between 24 and 48 h after irradiation. Bullet No survivors were obtained with dogs lethally irradiated with 600 rad by administering autogenic or allogenic DNA extracted from bone marrow, spleen or liver. Bullet Histocompatibility related to sex may play an important role in the bone-marrow graft. The lowest survival of C57BL mice was obtained when the donors were males and the recipients females. Bullet In radioprotection with foetal haemocytopoietic tissues, the donor's age represents one of the main factors. The best results were obtained in experiments on rats, with 19- to 20-day foetal liver (period of complete and maximum haemocytopoietic activity). The tissues mentioned below may be connected with the appearance of certain typical signs of secondary syndrome. Bullet Transfusion of blood or allogenic splenic cells did not influence noxiously the take of autogenic bone- marrow grafts in lethally irradiated dogs. Bullet After repeated irradiations and recovery of dogs (1-3 times) following treatment with autogenic bone- marrow, late chromosomal lesions were observed. (author)}
place = {IAEA}
year = {1969}
month = {Jul}
}