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Viability Tests for Fresh and Stored Haemopoietic Cells

Abstract

This paper reviews current methods of measurement of the viability of fresh and stored haemopoietic cells. The life expectancy of granulocytes and monocytes after transfusion can be studied by in-vitro labelling with {sup 3}H-DFP and subsequent autoradiography. The evaluation of data in about 30 patients with various haemopoietic conditions indicates a wide variation of the disappearance half-time of granulocytes. {sup 3}H-cytidine labels essentially all lymphocytes in vitro, predominantly in their RNA. Transfusion of {sup 3}H-cytidine-labelled lymphocytes enables one to measure the lower limit of their life-expectancy as well as their rate of RNA metabolism. If bone-marrow cells are labelled in vitro with {sup 3}H-thymidine and subsequently transfused, their capability to circulate, to reach the haemopoietic tissue of the host, to proliferate and to mature can be demonstrated. However, the repopulating capacity of frozen and thawed marrow is independent of the ability of {sup 3}H-TDR-labelled marrow cells to circulate, proliferate and mature. It is assumed that bone-marrow cells capable of repopulating depleted haemopoietic tissue are resting under steady-state conditions and can be labelled by means of {sup 3}H-TDR only using special conditions. Thus the only viability tests for fresh and stored bone-marrow cells at present appear to be bioassay methods at  More>>
Authors:
Fliedner, T. M. [1] 
  1. Abteilung fuer klinische Physiologie, Zentrum fuer Klinische Grundlagenforschung, Universitaet Ulm, Ulm, Federal Republic of Germany (Germany)
Publication Date:
Jul 15, 1969
Product Type:
Conference
Resource Relation:
Conference: Panel on Current Problems of Bone-Marrow Cell Transplantation with Special Emphasis on Conservation and Culture, Moscow (Russian Federation), 22-26 Jul 1968; Other Information: 33 refs., 7 figs., 2 tabs.; Related Information: In: Bone-Marrow Conservation, Culture and Transplantation. Proceedings of a Panel on Current Problems of Bone-Marrow Cell Transplantation with Special Emphasis on Conservation and Culture| 211 p.
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; AUTORADIOGRAPHY; BIOASSAY; BONE MARROW CELLS; CYTIDINE; IN VITRO; LABELLING; LYMPHOCYTES; METABOLISM; MONOCYTES; STEM CELLS; THYMIDINE; TRANSFUSIONS; TRITIUM
OSTI ID:
22192477
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA14M0714016966
Submitting Site:
INIS
Size:
page(s) 95-106
Announcement Date:
Feb 20, 2014

Citation Formats

Fliedner, T. M. Viability Tests for Fresh and Stored Haemopoietic Cells. IAEA: N. p., 1969. Web.
Fliedner, T. M. Viability Tests for Fresh and Stored Haemopoietic Cells. IAEA.
Fliedner, T. M. 1969. "Viability Tests for Fresh and Stored Haemopoietic Cells." IAEA.
@misc{etde_22192477,
title = {Viability Tests for Fresh and Stored Haemopoietic Cells}
author = {Fliedner, T. M.}
abstractNote = {This paper reviews current methods of measurement of the viability of fresh and stored haemopoietic cells. The life expectancy of granulocytes and monocytes after transfusion can be studied by in-vitro labelling with {sup 3}H-DFP and subsequent autoradiography. The evaluation of data in about 30 patients with various haemopoietic conditions indicates a wide variation of the disappearance half-time of granulocytes. {sup 3}H-cytidine labels essentially all lymphocytes in vitro, predominantly in their RNA. Transfusion of {sup 3}H-cytidine-labelled lymphocytes enables one to measure the lower limit of their life-expectancy as well as their rate of RNA metabolism. If bone-marrow cells are labelled in vitro with {sup 3}H-thymidine and subsequently transfused, their capability to circulate, to reach the haemopoietic tissue of the host, to proliferate and to mature can be demonstrated. However, the repopulating capacity of frozen and thawed marrow is independent of the ability of {sup 3}H-TDR-labelled marrow cells to circulate, proliferate and mature. It is assumed that bone-marrow cells capable of repopulating depleted haemopoietic tissue are resting under steady-state conditions and can be labelled by means of {sup 3}H-TDR only using special conditions. Thus the only viability tests for fresh and stored bone-marrow cells at present appear to be bioassay methods at the animal experimental level. The results indicate the need for the development of reliable viability tests for stem cells applicable in both experimental and clinical conditions. (author)}
place = {IAEA}
year = {1969}
month = {Jul}
}