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Effect of Phosphorus-32 Incorporated into Chick Embryo

Abstract

The bilogical effects of {sup 32}P on the viability and development of chick embryo were studied. 200,100 and 50 {mu}Ci per egg killed the embryos during incubation. Growth retardation preceded their death. 25 {mu}Ci per egg, however, did not cause mortality until hatching. An attempt was made to correlate the observed effects with the radiation dose and the number of {sup 32}P atoms incorporated into DNA and therefore, the frequencies of radiophosphorus atoms in the polynucleotide chains of DNA and RNA were determined under various conditions. Some biochemical consequences of the decay of {sup 32}P incorporated into ribonucleic acids were demonstrated. Structural changes of RNA were observed. Parallel with structural changes, functional alterations of various kinds of RNA molecules were demonstrated in isolated ribosomal systems. The decrease in protein synthesis by liver ribosomes was found to be proportional to the number of decayed intramolecular {sup 32}P atoms. The structural and functional changes of ribonucleic acids observed on molecular and sub-cellular levels could be attributed to nuclear transmutation of {sup 32}P. (author)
Authors:
Szabo, L. D.; Antoni, F.; Koeteles, G. J.; Holland, J.; Hidvegi, E. J.; Varteresz, V. [1] 
  1. Frederic Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, Budapest (Hungary)
Publication Date:
Jun 15, 1968
Product Type:
Conference
Report Number:
IAEA-PL-231/8
Resource Relation:
Conference: Panel on Biological Effects of Transmutation and Decay of Incorporated Radioisotopes, Vienna (Austria), 9-13 Oct 1967; Other Information: 35 refs., 2 tabs., 8 figs.; Related Information: In: Biological Effects of Transmutation and Decay of Incorporated Radioisotopes. Proceedings of a Panel| 258 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL GROWTH; BIOLOGICAL RADIATION EFFECTS; DEATH; DNA; EMBRYOS; HATCHING; INCUBATION; LIVER; MORTALITY; PHOSPHORUS 32; PROTEINS; RADIATION DOSES; RADIONUCLIDE KINETICS; RIBOSOMES; RNA
OSTI ID:
22190161
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA14M0680014646
Submitting Site:
INIS
Size:
page(s) 119-130
Announcement Date:
Feb 13, 2014

Citation Formats

Szabo, L. D., Antoni, F., Koeteles, G. J., Holland, J., Hidvegi, E. J., and Varteresz, V. Effect of Phosphorus-32 Incorporated into Chick Embryo. IAEA: N. p., 1968. Web.
Szabo, L. D., Antoni, F., Koeteles, G. J., Holland, J., Hidvegi, E. J., & Varteresz, V. Effect of Phosphorus-32 Incorporated into Chick Embryo. IAEA.
Szabo, L. D., Antoni, F., Koeteles, G. J., Holland, J., Hidvegi, E. J., and Varteresz, V. 1968. "Effect of Phosphorus-32 Incorporated into Chick Embryo." IAEA.
@misc{etde_22190161,
title = {Effect of Phosphorus-32 Incorporated into Chick Embryo}
author = {Szabo, L. D., Antoni, F., Koeteles, G. J., Holland, J., Hidvegi, E. J., and Varteresz, V.}
abstractNote = {The bilogical effects of {sup 32}P on the viability and development of chick embryo were studied. 200,100 and 50 {mu}Ci per egg killed the embryos during incubation. Growth retardation preceded their death. 25 {mu}Ci per egg, however, did not cause mortality until hatching. An attempt was made to correlate the observed effects with the radiation dose and the number of {sup 32}P atoms incorporated into DNA and therefore, the frequencies of radiophosphorus atoms in the polynucleotide chains of DNA and RNA were determined under various conditions. Some biochemical consequences of the decay of {sup 32}P incorporated into ribonucleic acids were demonstrated. Structural changes of RNA were observed. Parallel with structural changes, functional alterations of various kinds of RNA molecules were demonstrated in isolated ribosomal systems. The decrease in protein synthesis by liver ribosomes was found to be proportional to the number of decayed intramolecular {sup 32}P atoms. The structural and functional changes of ribonucleic acids observed on molecular and sub-cellular levels could be attributed to nuclear transmutation of {sup 32}P. (author)}
place = {IAEA}
year = {1968}
month = {Jun}
}