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Autoradiography at Cell and Chromosome Level in the Study of Multiplication and Cytodifferentiation of Haematopoietic Tissue

Abstract

Haematopoietic tissue proliferates by the mitotic activity of the youngest cells. The first data on the proliferation of blood cells recorded by use of the stathmokinetic technique was followed by more detailed information obtained with autoradiographic studies and by using special precursors of DNA such as thymidine. It has been observed that in the bone marrow in progressive myeloid and erythroid maturation, proliferative capacity decreases progressively until it stops altogether at the myelocytic and polychromatic erythroblastic stage. For chronic myeloid leukaemia the labelling index of each proliferating cell type is similar to the values of the corresponding normal cells. In 1958 we used tritiated thymidine to study many cases of acute leukaemia, that is those with a complete differentiation block and a very high blast content, and we found a clear fall in proliferative capacity of these blast cells. The same results have been obtained in other laboratories and now, quite the contrary to what was thought a few years ago, namely that leukaemic cells grew faster than normal cells, we know that the rate of growth of these cells is often much slower than the corresponding normal cells.
Authors:
Gavosto, F. [1] 
  1. Instituto di Clinica Medica, University of Turin (Italy)
Publication Date:
Jul 15, 1967
Product Type:
Conference
Resource Relation:
Conference: Panel on the Effects of Various Types of Ionizing Radiations from Different Sources on Haematopoietic Tissue, Vienna (Austria), 17-20 May 1966; Other Information: 17 refs., 2 figs.; Related Information: In: Effects of Ionizing Radiations on the Haematopoietic Tissue. Proceedings of a Panel on the Effects of Various Types of Ionizing Radiations from Different Sources on Haematopoietic Tissue| 174 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; AUTORADIOGRAPHY; BIOLOGICAL RADIATION EFFECTS; BLOOD CELLS; BLOOD FORMATION; BONE MARROW; CELL DIFFERENTIATION; CELL PROLIFERATION; CHROMOSOMES; DNA; LABELLING; LEUKEMIA; STEM CELLS; THYMIDINE
OSTI ID:
22190124
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Contract Number:
Contract 016-62-1 BIOI; 061-66-3-BIO
Other Identifying Numbers:
TRN: XA14M0643014609
Submitting Site:
INIS
Size:
page(s) 38-42
Announcement Date:
Feb 13, 2014

Citation Formats

Gavosto, F. Autoradiography at Cell and Chromosome Level in the Study of Multiplication and Cytodifferentiation of Haematopoietic Tissue. IAEA: N. p., 1967. Web.
Gavosto, F. Autoradiography at Cell and Chromosome Level in the Study of Multiplication and Cytodifferentiation of Haematopoietic Tissue. IAEA.
Gavosto, F. 1967. "Autoradiography at Cell and Chromosome Level in the Study of Multiplication and Cytodifferentiation of Haematopoietic Tissue." IAEA.
@misc{etde_22190124,
title = {Autoradiography at Cell and Chromosome Level in the Study of Multiplication and Cytodifferentiation of Haematopoietic Tissue}
author = {Gavosto, F.}
abstractNote = {Haematopoietic tissue proliferates by the mitotic activity of the youngest cells. The first data on the proliferation of blood cells recorded by use of the stathmokinetic technique was followed by more detailed information obtained with autoradiographic studies and by using special precursors of DNA such as thymidine. It has been observed that in the bone marrow in progressive myeloid and erythroid maturation, proliferative capacity decreases progressively until it stops altogether at the myelocytic and polychromatic erythroblastic stage. For chronic myeloid leukaemia the labelling index of each proliferating cell type is similar to the values of the corresponding normal cells. In 1958 we used tritiated thymidine to study many cases of acute leukaemia, that is those with a complete differentiation block and a very high blast content, and we found a clear fall in proliferative capacity of these blast cells. The same results have been obtained in other laboratories and now, quite the contrary to what was thought a few years ago, namely that leukaemic cells grew faster than normal cells, we know that the rate of growth of these cells is often much slower than the corresponding normal cells.}
place = {IAEA}
year = {1967}
month = {Jul}
}