Abstract
It was of interest to us to study the metabolism of epinephrine in normal human subjects. Studies of epinephrine metabolism in animals have indicated the possible routes of its metabolism, but the doses, both of radioactivity and of the catechol amine, have been greater than the levels which are safe to use in humans. Also earlier studies of the metabolism of this hormone in humans have been limited by the relatively low specific activity of the labelled epinephrine. The use of tritium-labelled epinephrine (7-H{sup 3}-dl-epinephrine- d-bitartrate) has made possible more complete recovery of the administered radioactivity, and the higher specific activity (greater than 300 {mu}c/mg) has allowed isolation and quantification of new metabolites. In twelve normal human males 92.6 {+-} 7.7% of the tritium in the infused H{sup 3}-epinephrine was excreted into the urine within 54 h following the infusion of the tritium-labelled epinephrine. The tritium-labelled urinary constituents were as follows: unchanged epinephrine 6.8 {+-} 1.5%, free metanephrine 5.2 {+-} 1.1%, metanephrine glucuronide 6.0 {+-} 1.5%, metanephrine sulphate 29.5 {+-} 9.0%, 3-methoxy-4-hydroxyphenylglycol 7.1 {+-} 1.4%, free and conjugated 3,4-dihydroxymandelic acid 1.6 {+-} 0-6% and 3-methoxy-4-hydroxymandelic acid 41.2 {+-} 8.4%. As demonstrated by this and related experiments, the biological application of
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Labrosse, E. H.;
Axelrod, J.;
Kopin, I. J.;
Kety, S. S.
[1]
- National Institute of Health, Bethesda, MD (United States)
Citation Formats
Labrosse, E. H., Axelrod, J., Kopin, I. J., and Kety, S. S.
The Metabolism of Tritium-Labelled Epinephrine in Man; Metabolisme de l'Adrenaline Tritiee dans l'Organisme Humain; 041c 0435 0414 ; Metabolismo de la Adrenalina Marcada con Tritio en el Hombre.
IAEA: N. p.,
1962.
Web.
Labrosse, E. H., Axelrod, J., Kopin, I. J., & Kety, S. S.
The Metabolism of Tritium-Labelled Epinephrine in Man; Metabolisme de l'Adrenaline Tritiee dans l'Organisme Humain; 041c 0435 0414 ; Metabolismo de la Adrenalina Marcada con Tritio en el Hombre.
IAEA.
Labrosse, E. H., Axelrod, J., Kopin, I. J., and Kety, S. S.
1962.
"The Metabolism of Tritium-Labelled Epinephrine in Man; Metabolisme de l'Adrenaline Tritiee dans l'Organisme Humain; 041c 0435 0414 ; Metabolismo de la Adrenalina Marcada con Tritio en el Hombre."
IAEA.
@misc{etde_22190115,
title = {The Metabolism of Tritium-Labelled Epinephrine in Man; Metabolisme de l'Adrenaline Tritiee dans l'Organisme Humain; 041c 0435 0414 ; Metabolismo de la Adrenalina Marcada con Tritio en el Hombre}
author = {Labrosse, E. H., Axelrod, J., Kopin, I. J., and Kety, S. S.}
abstractNote = {It was of interest to us to study the metabolism of epinephrine in normal human subjects. Studies of epinephrine metabolism in animals have indicated the possible routes of its metabolism, but the doses, both of radioactivity and of the catechol amine, have been greater than the levels which are safe to use in humans. Also earlier studies of the metabolism of this hormone in humans have been limited by the relatively low specific activity of the labelled epinephrine. The use of tritium-labelled epinephrine (7-H{sup 3}-dl-epinephrine- d-bitartrate) has made possible more complete recovery of the administered radioactivity, and the higher specific activity (greater than 300 {mu}c/mg) has allowed isolation and quantification of new metabolites. In twelve normal human males 92.6 {+-} 7.7% of the tritium in the infused H{sup 3}-epinephrine was excreted into the urine within 54 h following the infusion of the tritium-labelled epinephrine. The tritium-labelled urinary constituents were as follows: unchanged epinephrine 6.8 {+-} 1.5%, free metanephrine 5.2 {+-} 1.1%, metanephrine glucuronide 6.0 {+-} 1.5%, metanephrine sulphate 29.5 {+-} 9.0%, 3-methoxy-4-hydroxyphenylglycol 7.1 {+-} 1.4%, free and conjugated 3,4-dihydroxymandelic acid 1.6 {+-} 0-6% and 3-methoxy-4-hydroxymandelic acid 41.2 {+-} 8.4%. As demonstrated by this and related experiments, the biological application of tritium-labelled epinephrine has made possible a much more complete understanding of the metabolism of this important hormone in humans. (author) [French] L'etude du metabolisme de l'adrenaline dans l'organisme de l'homme normal a presente un reel interet. Des etudes faites sur les animaux avaient montre quelles etaient les voies possibles de ce metabolisme, mais les doses de radioactivite et de catecholamine employees etaient superieures a celles qui peuvent etre administrees a l'homme sans presenter de danger. Les etudes anterieures sur le metabolisme de cette hormone chez l'homme avaient ete limitees par le fait que l'adrenaline marquee avait une activite specifique relativement faible. Le recours a l'adrenaline tritiee (7-{sup 3}H-dl-adrenaline-d-bitartrate) a permis une meilleure recuperation des substances radioactives administrees, et grace a l'activite specifique plus elevee (superieure a 300 microcuries par milligramme) il a ete possible d'isoler de nouveaux metabolites et de determiner leur quantite. Chez 12 sujets males normaux, 92,6 {+-} 7,7% du tritium contenu dans l'adrenaline-3 infusee a ete evacue dans l'urine dans les 54 heures qui ont suivi l'infusion de l'adrenaline tritiee. L'urine contenait les substances tritiees suivantes: adrenaline inchangee, 6,8 {+-} 1,5%; metanephrine libre, 5,2 {+-} 1,1%; glycuronate de metanephrine, 6,0 {+-} 1,5%; sulfate de metanephrine, 29,5 {+-} 9,0%; metoxy-3-hydroxy-4-phenylglycole, 7,1 {+-} 1,4%; acide dihydroxy-3, 4-mandelique libre et conjugue, 1,6 {+-} 0,6%; acide metoxy-3-hydroxy-4-mandelique, 41,2 {+-} 8,4%. Comme le montrent ces experiences et d'autres se rapportant au meme domaine, l'application biologique de l'adrenaline tritiee a permis de mieux comprendre le metabolisme de cette importante hormone de l'organisme humain. (author) [Spanish] Los autores tenian interes en estudiar el metabolismo de la adrenalina en seres humanos normales. Las investigaciones realizadas en animales indicaron algunos ciclos metabolicos posibles, pero las dosis de radiactividad y de aminopirocatequina empleadas eran superiores a las que el ser humano puede soportar sin peligro. Asimismo, los estudios anteriores acerca del metabolismo de esta hormona en el hombre quedaron limitados por la actividad especifica relativamente baja de la adrenalina marcada. El empleo de adrenalina tritiada (d-bitartrato de 7-{sup 3}H-dl-adtenalma) ha permitido recuperar un porcentaje mayor de la radioactividad administrada, y su actividad especifica mas elevada (mas de 300 {mu}curies/mg) ha hecho posible aislar y determinar cuantitativamente nuevos metabolitos. En 12 varones normales, el 92,6 {+-} 7,7% del tritio de la adrenalina-3 administrada se excreto por la orina al cabo de 54 h. Este tritio estaba repartido entre los siguientes compuestos : adrenalina sin alterar 6,8 {+-} 1,5%, metanefrina libre 5,2 {+-} 1,1%, glucuronato de metanefrina 6,0 {+-} 1,5%, sulfato de metanefrina 29,5 {+-} 9,0%, 3-metoxi-4-hidroxifenilglicol 7,1 {+-} 1,4%, acido 3,4-dihidroximandelico libre y conjugado 1,6 {+-} 0,6% y acido 3-metoxi-4-hidroximandelico 41,2 {+-} 8,4%. Como demuestran estos experimentos y otros similares, el empleo de la adrenalina tritiada permite formarse una idea mucho mas clara del metabolismo de esta importante hormona en el hombre. (author) [Russian] Predstavljaet interes issledovanie metabolizma adrenalina u cheloveka. Izuchenie metabolizma adrena- lina u zhivotnyh pokazalo vozmozhnye korni ego metabolizma, no dozy radio- aktivnosti i katehin amina byli vyshe urovnej, javljajushhihsja bezopasnymi dlja cheloveka. Rannie issledovanija metabolizma jetogo gormona u cheloveka ogranivalis' otnositel'no nizkoj udel'noj aktivnost'ju mechenogo adrjonalina. Ispol'- zovanie tritirovannogo adrenalina (7-N{sup 3}-s1)-adrenalin-s1-kilaja sol') pozvolilo provesti bolee polnoe vosstanovlenie vvodimoj raidoaktivnosti, a bolee vyso- kaja udel'naja aktivnost' (vyshe 300 {mu}c/mg) pozvolila izolirovat' i opredelit' kolichestvo novyh metabolitev. Y 12 muzhchin 92,6 {+-} 7,7% tritija v vvedennom N{sup 3}-adrenaline vyshlo v mochu cherez 54 chasa posle vvedenija tritirovannogo adrenalina. Mechennyj tritiem sostav mochi okazalsja sledujushhim: neizmenennyj adrenalin 6,8 {+-} 1,5%, svobod- nyj metanefrin (metanephrino) 5,2 {+-} 1,1%, metanefrinovyj gljukuronid (glucuronide) 6,0 {+-} 1,5 % , metanefrinovyj sul'fat 29,5 {+-}9,0%, Z-metoksi-4- gidrooksifenilglikol' 7,1 {+-} 1 , 4 % , svobodnaja i soprjazhennaja 3,4-dioksimin- dal'naja kislota 1,6 {+-} 0,6% i Z-metoksi-4-oksimindal'naja kislota 41,2 {+-} 8,4%. Podtverzhdennoe jetim i drugimi podobnymi jeksperimentami biologicheskoe primenenie mechennogo tritiem adrenalina dalo vozmozhnost' bolee polnogo ponimanija metabolizma jetogo vazhnogo gormona u cheloveka. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}
title = {The Metabolism of Tritium-Labelled Epinephrine in Man; Metabolisme de l'Adrenaline Tritiee dans l'Organisme Humain; 041c 0435 0414 ; Metabolismo de la Adrenalina Marcada con Tritio en el Hombre}
author = {Labrosse, E. H., Axelrod, J., Kopin, I. J., and Kety, S. S.}
abstractNote = {It was of interest to us to study the metabolism of epinephrine in normal human subjects. Studies of epinephrine metabolism in animals have indicated the possible routes of its metabolism, but the doses, both of radioactivity and of the catechol amine, have been greater than the levels which are safe to use in humans. Also earlier studies of the metabolism of this hormone in humans have been limited by the relatively low specific activity of the labelled epinephrine. The use of tritium-labelled epinephrine (7-H{sup 3}-dl-epinephrine- d-bitartrate) has made possible more complete recovery of the administered radioactivity, and the higher specific activity (greater than 300 {mu}c/mg) has allowed isolation and quantification of new metabolites. In twelve normal human males 92.6 {+-} 7.7% of the tritium in the infused H{sup 3}-epinephrine was excreted into the urine within 54 h following the infusion of the tritium-labelled epinephrine. The tritium-labelled urinary constituents were as follows: unchanged epinephrine 6.8 {+-} 1.5%, free metanephrine 5.2 {+-} 1.1%, metanephrine glucuronide 6.0 {+-} 1.5%, metanephrine sulphate 29.5 {+-} 9.0%, 3-methoxy-4-hydroxyphenylglycol 7.1 {+-} 1.4%, free and conjugated 3,4-dihydroxymandelic acid 1.6 {+-} 0-6% and 3-methoxy-4-hydroxymandelic acid 41.2 {+-} 8.4%. As demonstrated by this and related experiments, the biological application of tritium-labelled epinephrine has made possible a much more complete understanding of the metabolism of this important hormone in humans. (author) [French] L'etude du metabolisme de l'adrenaline dans l'organisme de l'homme normal a presente un reel interet. Des etudes faites sur les animaux avaient montre quelles etaient les voies possibles de ce metabolisme, mais les doses de radioactivite et de catecholamine employees etaient superieures a celles qui peuvent etre administrees a l'homme sans presenter de danger. Les etudes anterieures sur le metabolisme de cette hormone chez l'homme avaient ete limitees par le fait que l'adrenaline marquee avait une activite specifique relativement faible. Le recours a l'adrenaline tritiee (7-{sup 3}H-dl-adrenaline-d-bitartrate) a permis une meilleure recuperation des substances radioactives administrees, et grace a l'activite specifique plus elevee (superieure a 300 microcuries par milligramme) il a ete possible d'isoler de nouveaux metabolites et de determiner leur quantite. Chez 12 sujets males normaux, 92,6 {+-} 7,7% du tritium contenu dans l'adrenaline-3 infusee a ete evacue dans l'urine dans les 54 heures qui ont suivi l'infusion de l'adrenaline tritiee. L'urine contenait les substances tritiees suivantes: adrenaline inchangee, 6,8 {+-} 1,5%; metanephrine libre, 5,2 {+-} 1,1%; glycuronate de metanephrine, 6,0 {+-} 1,5%; sulfate de metanephrine, 29,5 {+-} 9,0%; metoxy-3-hydroxy-4-phenylglycole, 7,1 {+-} 1,4%; acide dihydroxy-3, 4-mandelique libre et conjugue, 1,6 {+-} 0,6%; acide metoxy-3-hydroxy-4-mandelique, 41,2 {+-} 8,4%. Comme le montrent ces experiences et d'autres se rapportant au meme domaine, l'application biologique de l'adrenaline tritiee a permis de mieux comprendre le metabolisme de cette importante hormone de l'organisme humain. (author) [Spanish] Los autores tenian interes en estudiar el metabolismo de la adrenalina en seres humanos normales. Las investigaciones realizadas en animales indicaron algunos ciclos metabolicos posibles, pero las dosis de radiactividad y de aminopirocatequina empleadas eran superiores a las que el ser humano puede soportar sin peligro. Asimismo, los estudios anteriores acerca del metabolismo de esta hormona en el hombre quedaron limitados por la actividad especifica relativamente baja de la adrenalina marcada. El empleo de adrenalina tritiada (d-bitartrato de 7-{sup 3}H-dl-adtenalma) ha permitido recuperar un porcentaje mayor de la radioactividad administrada, y su actividad especifica mas elevada (mas de 300 {mu}curies/mg) ha hecho posible aislar y determinar cuantitativamente nuevos metabolitos. En 12 varones normales, el 92,6 {+-} 7,7% del tritio de la adrenalina-3 administrada se excreto por la orina al cabo de 54 h. Este tritio estaba repartido entre los siguientes compuestos : adrenalina sin alterar 6,8 {+-} 1,5%, metanefrina libre 5,2 {+-} 1,1%, glucuronato de metanefrina 6,0 {+-} 1,5%, sulfato de metanefrina 29,5 {+-} 9,0%, 3-metoxi-4-hidroxifenilglicol 7,1 {+-} 1,4%, acido 3,4-dihidroximandelico libre y conjugado 1,6 {+-} 0,6% y acido 3-metoxi-4-hidroximandelico 41,2 {+-} 8,4%. Como demuestran estos experimentos y otros similares, el empleo de la adrenalina tritiada permite formarse una idea mucho mas clara del metabolismo de esta importante hormona en el hombre. (author) [Russian] Predstavljaet interes issledovanie metabolizma adrenalina u cheloveka. Izuchenie metabolizma adrena- lina u zhivotnyh pokazalo vozmozhnye korni ego metabolizma, no dozy radio- aktivnosti i katehin amina byli vyshe urovnej, javljajushhihsja bezopasnymi dlja cheloveka. Rannie issledovanija metabolizma jetogo gormona u cheloveka ogranivalis' otnositel'no nizkoj udel'noj aktivnost'ju mechenogo adrjonalina. Ispol'- zovanie tritirovannogo adrenalina (7-N{sup 3}-s1)-adrenalin-s1-kilaja sol') pozvolilo provesti bolee polnoe vosstanovlenie vvodimoj raidoaktivnosti, a bolee vyso- kaja udel'naja aktivnost' (vyshe 300 {mu}c/mg) pozvolila izolirovat' i opredelit' kolichestvo novyh metabolitev. Y 12 muzhchin 92,6 {+-} 7,7% tritija v vvedennom N{sup 3}-adrenaline vyshlo v mochu cherez 54 chasa posle vvedenija tritirovannogo adrenalina. Mechennyj tritiem sostav mochi okazalsja sledujushhim: neizmenennyj adrenalin 6,8 {+-} 1,5%, svobod- nyj metanefrin (metanephrino) 5,2 {+-} 1,1%, metanefrinovyj gljukuronid (glucuronide) 6,0 {+-} 1,5 % , metanefrinovyj sul'fat 29,5 {+-}9,0%, Z-metoksi-4- gidrooksifenilglikol' 7,1 {+-} 1 , 4 % , svobodnaja i soprjazhennaja 3,4-dioksimin- dal'naja kislota 1,6 {+-} 0,6% i Z-metoksi-4-oksimindal'naja kislota 41,2 {+-} 8,4%. Podtverzhdennoe jetim i drugimi podobnymi jeksperimentami biologicheskoe primenenie mechennogo tritiem adrenalina dalo vozmozhnost' bolee polnogo ponimanija metabolizma jetogo vazhnogo gormona u cheloveka. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}