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Application of Tritiated Compounds to the Midge Chironomus and some Aspects of the Metabolism of Salivary Gland Chromosomes; Emploi de Composes Trities pour l'Etude du Chironome et de Certains Aspects du Metabolisme des Chromosomes des Glandes Salivaires; 041f 0440 0438 043c 0414 ; Aplicacion de Compuestos Tritiados a la Mosca Cbironomus y Algunos Aspectos del Metabolismo en los Cromosomas de las Glandulas Salivares

Conference:

Abstract

The investigations were carried out on the salivary gland chromosomes of Chironomus tentons. Tritiated compounds (H{sup 3}-thymidine, H{sup 3}-uridine, H{sup 3}-amino-acids), injected into the haemolymph of the larvae should indicate the place of incorporation within the giant chromosomes. After fixation of the salivary glands autoradiographs of the squash-preparations were made. The autoradiographs show that giant chromosomes are most suitable to localize the activity at chromosomal structures with high resolution. DNA-synthesis (thymidine), RNA-synthesis (uridine) and protein-synthesis within the cell could be followed by determining the time and approximatively the quantity of incorporation: Contrary to the protein-synthesis, the DNA-synthesis and the RNA-synthesis are restricted to the chromosomes. The essential physiological activity of the chromosomes seems to be represented by RNA synthesis which takes place at certain distinct loci (nuceolar organizers, ''Balbiani-rings'', puffs, and other chromosomal bands). The report discusses some features of RNA synthesis. (author) [French] Les recherches ont porte sur les chromosomes des glandes salivaires du chironomus tentans. On a injecte les composes trities (thymidine, uridine, acides amines) dans l'hemolymphe de la larve en vue de determiner le lieu d'incorporation dans les chromosomes geants. Apres fixation par les glandes salivaires, on a procede a des autoradiographies de preparations broyees. Ces autoradiographies  More>>
Authors:
Pelling, C. [1] 
  1. Max-Planck-Institut fuer Biologie, Tuebingen, Federal Republic of Germany (Germany)
Publication Date:
Feb 15, 1962
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences, Vienna (Austria), 3-10 May 1961; Other Information: 7 refs., 8 figs.; Related Information: In: Tritium in the Physical and Biological Sciences. Vol. II. Proceedings of the Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences| 456 p.
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; AMINO ACIDS; CHROMOSOMES; DNA; IMAGES; LARVAE; METABOLISM; PROTEINS; RNA; SALIVARY GLANDS; SYNTHESIS; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; URIDINE
OSTI ID:
22190108
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3711014593
Submitting Site:
INIS
Size:
page(s) 327-334
Announcement Date:
Feb 14, 2014

Conference:

Citation Formats

Pelling, C. Application of Tritiated Compounds to the Midge Chironomus and some Aspects of the Metabolism of Salivary Gland Chromosomes; Emploi de Composes Trities pour l'Etude du Chironome et de Certains Aspects du Metabolisme des Chromosomes des Glandes Salivaires; 041f 0440 0438 043c 0414 ; Aplicacion de Compuestos Tritiados a la Mosca Cbironomus y Algunos Aspectos del Metabolismo en los Cromosomas de las Glandulas Salivares. IAEA: N. p., 1962. Web.
Pelling, C. Application of Tritiated Compounds to the Midge Chironomus and some Aspects of the Metabolism of Salivary Gland Chromosomes; Emploi de Composes Trities pour l'Etude du Chironome et de Certains Aspects du Metabolisme des Chromosomes des Glandes Salivaires; 041f 0440 0438 043c 0414 ; Aplicacion de Compuestos Tritiados a la Mosca Cbironomus y Algunos Aspectos del Metabolismo en los Cromosomas de las Glandulas Salivares. IAEA.
Pelling, C. 1962. "Application of Tritiated Compounds to the Midge Chironomus and some Aspects of the Metabolism of Salivary Gland Chromosomes; Emploi de Composes Trities pour l'Etude du Chironome et de Certains Aspects du Metabolisme des Chromosomes des Glandes Salivaires; 041f 0440 0438 043c 0414 ; Aplicacion de Compuestos Tritiados a la Mosca Cbironomus y Algunos Aspectos del Metabolismo en los Cromosomas de las Glandulas Salivares." IAEA.
@misc{etde_22190108,
title = {Application of Tritiated Compounds to the Midge Chironomus and some Aspects of the Metabolism of Salivary Gland Chromosomes; Emploi de Composes Trities pour l'Etude du Chironome et de Certains Aspects du Metabolisme des Chromosomes des Glandes Salivaires; 041f 0440 0438 043c 0414 ; Aplicacion de Compuestos Tritiados a la Mosca Cbironomus y Algunos Aspectos del Metabolismo en los Cromosomas de las Glandulas Salivares}
author = {Pelling, C.}
abstractNote = {The investigations were carried out on the salivary gland chromosomes of Chironomus tentons. Tritiated compounds (H{sup 3}-thymidine, H{sup 3}-uridine, H{sup 3}-amino-acids), injected into the haemolymph of the larvae should indicate the place of incorporation within the giant chromosomes. After fixation of the salivary glands autoradiographs of the squash-preparations were made. The autoradiographs show that giant chromosomes are most suitable to localize the activity at chromosomal structures with high resolution. DNA-synthesis (thymidine), RNA-synthesis (uridine) and protein-synthesis within the cell could be followed by determining the time and approximatively the quantity of incorporation: Contrary to the protein-synthesis, the DNA-synthesis and the RNA-synthesis are restricted to the chromosomes. The essential physiological activity of the chromosomes seems to be represented by RNA synthesis which takes place at certain distinct loci (nuceolar organizers, ''Balbiani-rings'', puffs, and other chromosomal bands). The report discusses some features of RNA synthesis. (author) [French] Les recherches ont porte sur les chromosomes des glandes salivaires du chironomus tentans. On a injecte les composes trities (thymidine, uridine, acides amines) dans l'hemolymphe de la larve en vue de determiner le lieu d'incorporation dans les chromosomes geants. Apres fixation par les glandes salivaires, on a procede a des autoradiographies de preparations broyees. Ces autoradiographies montrent que ce sont les chromosomes geants qui permettent le mieux de localiser,- avec une forte resolution, l'activite au niveau chromosomique. L'auteur a pu suivre la synthese de l'ADN (thymidine), la synthese de l'ARN (uridine) et la synthese des proteines a l'interieur de la cellule en determinant le temps et le taux approximatif d'incorporation. A l'inverse de la synthese des proteines, la synthese de l'ADN et celle de l'ARN sont limitees aux chromosomes. L'activite physiologique essentielle des chromosomes semble representee par la synthese de l'ARN, qui se produit en certains 'loci' distincts ('organiseurs' nucleaires, anneaux de Balbiani, nodules et bandes chromosomiques). L'auteur examine certains aspects caracteristiques de la synthese de l'ARN. (author) [Spanish] l.os autores realizaron sus investigaciones con los cromosomas de las glandulas salivares del Ghironomus tentans. La inyeccion do compuestos tritiados (timidina-{sup 3}H, uridina-{sup 3}H, aminoacidos {sup 3}H) en la hemolinfa de las larvas permite determinar en que lugar de los cromosomas gigantes se produce la incorporacion. Una vez fijadas las glandulas salivares, los autores obtuvieron autorradiografias de las preparaciones trituradas. Estas autorradiografias demuestran que los cromosomas gigantes son los mas adecuados para localizar la radiactividad en las estructuras cromosomicas con un grado de resolucion muy elevado. Determinando el tiempo y el grado aproximado de incorporacion, los autores han conseguido seguir en la celula la sintesis del ADN (timidina), del ARN (uridina) y de las proteinas. Las dos primeras tienen lugar exclusivamente en los cromosomas, contrariamente a lo que ocurre para la sintesis de las proteinas. Al parecer, la actividad fisiologica esencial de los cromosomas consiste en la sintesis del ARN, que se produce en ciertos lugares determinados (organizadores nucleares, anillos de Balbiani, y otras bandas cromosomicas). La memoria discute algunos aspectos de la sintesis del ARN. (author) [Russian] Provo- dilis' issledovanija hromosom sljunnoj zhelezy Chironomus tentans. Tretiro- vannye soedinenija ({sup 3}N-timidin, {sup 3}N-uridin, {sup 3}N-aminokisloty), vvedennye v gemolimf lichinki, dolzhny ukazyvat' mesta pogloshhenija v bol'shih hromosomah. Posle fiksacii sljunnyh zhelez byli sdelany radioavtogrammy mjagkoj massy. Radioavtogrammy pokazyvajut, chto bol'shie hromosomy javljajutsja bolee pod- hodjashhimi dlja lokalizacii aktivnosti u hromosomnyh struktur s vysokoj stepen'ju razdelenija. Sintez DNK (timidin), sintez RNK (uridin) i proteinovyj sintez v kletke mozhno prosledit' putem opredelenija vremeni i priblizitel'- nogo kolichestva pogloshhenija. V protivopolozhnost' proteinovomu sintezu sintez-DNK i sintez RNK javlja- jutsja ogranichennymi dlja hromosom. Sushhestvennaja fiziologicheskaja aktivnost' hromosom, vidimo, predstavlena sintezom RNK, kotoryj imeet mesto pri opre- delennyh otchetlivyh mestopolozhenijah (nukleoljarnye obrazovateli, kol'ca 'Balbiani' odutlovatosti i drugie hromosomye svjazi). I doklade rassmatrivajutsja nekotorye osobennosti sinteza NIK. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}