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Tritiated Thymidine as Tracer in DNA Metabolism and Cell Dynamics of Experimental Myeloid Leukaemia; Emploi de la Thymidine Tritiee comme Indicateur pour l'Etude du Metabolisme de l'ADN et de la Dynamique des Cellules dans la Leucemie Myeloide Experimentale; 0422 0440 0438 0442 0414 ; Empleo de la Timidina Tritiada como Indicador para Estudiar el Metabolismo del Acido Desoxirribonucleico y la Dinamica Celular en la Leucemia Mieloide Experimental

Conference:

Abstract

Tritium has been used as an isotopic tracer in a variety of biological problems in Israel. We wish to report, in particular, some findings in which tritiated thymidine (TH{sup 3}) has been used to follow the cell dynamics in experimental myeloid leukaemia and also to investigate the mechanism of its incorporation into the DNA of these and Ehrlich ascites tumour cells. The leukaemic cells were labelled in-vivo by injecting the TH{sup 3} into the jugular vein. The dose was 1 pc/g/rat. The rate of appearance of the labelled cells in the peripheral blood and in the ascitic tumour of the animal, was estimated. In other experiments the rate of the dilution of the label in the nuclei was evaluated and thus it was possible to estimate the cellular doubling time in the myelocyte population. The dynamics of transfused leukaemia cells were investigated by injecting labelled myelocytes into the jugular vein of normal and leukaemic rats. Their rate of disappearance from the blood was measured. Various organs were examined for the labelled cells and it was found that soon after injection the cells were mainly trapped by the lungs, later by the spleen and to a lesser extent by the liver.  More>>
Authors:
Zajicek, G.; Rosin, A.; Gross, J. [1] 
  1. Department of Experimental Medicine and Cancer Research, Hebrew University, Hadassah Medical School, Jerusalem (Israel)
Publication Date:
Feb 15, 1962
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences, Vienna (Austria), 3-10 May 1961; Other Information: 12 refs., 5 figs.; Related Information: In: Tritium in the Physical and Biological Sciences. Vol. II. Proceedings of the Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences| 456 p.
Subject:
60 APPLIED LIFE SCIENCES; ASCITES; BIOSYNTHESIS; BLOOD; CONCENTRATION RATIO; DNA; IN VITRO; IN VIVO; INCUBATION; LABELLING; LEUKEMIA; LIVER; LUNGS; METABOLISM; MITOSIS; RATS; SPLEEN; THYMIDINE; TRACER TECHNIQUES; TRITIUM; VEINS
OSTI ID:
22190106
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3709014591
Submitting Site:
INIS
Size:
page(s) 291-298
Announcement Date:
Feb 14, 2014

Conference:

Citation Formats

Zajicek, G., Rosin, A., and Gross, J. Tritiated Thymidine as Tracer in DNA Metabolism and Cell Dynamics of Experimental Myeloid Leukaemia; Emploi de la Thymidine Tritiee comme Indicateur pour l'Etude du Metabolisme de l'ADN et de la Dynamique des Cellules dans la Leucemie Myeloide Experimentale; 0422 0440 0438 0442 0414 ; Empleo de la Timidina Tritiada como Indicador para Estudiar el Metabolismo del Acido Desoxirribonucleico y la Dinamica Celular en la Leucemia Mieloide Experimental. IAEA: N. p., 1962. Web.
Zajicek, G., Rosin, A., & Gross, J. Tritiated Thymidine as Tracer in DNA Metabolism and Cell Dynamics of Experimental Myeloid Leukaemia; Emploi de la Thymidine Tritiee comme Indicateur pour l'Etude du Metabolisme de l'ADN et de la Dynamique des Cellules dans la Leucemie Myeloide Experimentale; 0422 0440 0438 0442 0414 ; Empleo de la Timidina Tritiada como Indicador para Estudiar el Metabolismo del Acido Desoxirribonucleico y la Dinamica Celular en la Leucemia Mieloide Experimental. IAEA.
Zajicek, G., Rosin, A., and Gross, J. 1962. "Tritiated Thymidine as Tracer in DNA Metabolism and Cell Dynamics of Experimental Myeloid Leukaemia; Emploi de la Thymidine Tritiee comme Indicateur pour l'Etude du Metabolisme de l'ADN et de la Dynamique des Cellules dans la Leucemie Myeloide Experimentale; 0422 0440 0438 0442 0414 ; Empleo de la Timidina Tritiada como Indicador para Estudiar el Metabolismo del Acido Desoxirribonucleico y la Dinamica Celular en la Leucemia Mieloide Experimental." IAEA.
@misc{etde_22190106,
title = {Tritiated Thymidine as Tracer in DNA Metabolism and Cell Dynamics of Experimental Myeloid Leukaemia; Emploi de la Thymidine Tritiee comme Indicateur pour l'Etude du Metabolisme de l'ADN et de la Dynamique des Cellules dans la Leucemie Myeloide Experimentale; 0422 0440 0438 0442 0414 ; Empleo de la Timidina Tritiada como Indicador para Estudiar el Metabolismo del Acido Desoxirribonucleico y la Dinamica Celular en la Leucemia Mieloide Experimental}
author = {Zajicek, G., Rosin, A., and Gross, J.}
abstractNote = {Tritium has been used as an isotopic tracer in a variety of biological problems in Israel. We wish to report, in particular, some findings in which tritiated thymidine (TH{sup 3}) has been used to follow the cell dynamics in experimental myeloid leukaemia and also to investigate the mechanism of its incorporation into the DNA of these and Ehrlich ascites tumour cells. The leukaemic cells were labelled in-vivo by injecting the TH{sup 3} into the jugular vein. The dose was 1 pc/g/rat. The rate of appearance of the labelled cells in the peripheral blood and in the ascitic tumour of the animal, was estimated. In other experiments the rate of the dilution of the label in the nuclei was evaluated and thus it was possible to estimate the cellular doubling time in the myelocyte population. The dynamics of transfused leukaemia cells were investigated by injecting labelled myelocytes into the jugular vein of normal and leukaemic rats. Their rate of disappearance from the blood was measured. Various organs were examined for the labelled cells and it was found that soon after injection the cells were mainly trapped by the lungs, later by the spleen and to a lesser extent by the liver. After 24 h no labelled cells were detectable in any of the organs. Information was thus obtained on the fate of the leukaemic myelocytes in various organs of the normal and leukaemic animals. In in-vitro experiments, TH{sup 3} was added to the cell suspension in a concentration of 1 p.c/ml. In the course of the in-vitro labelling it was observed that the number of labelled cells was 40 times higher than the number of mitoses. (The same was found also after administering the TH{sup 3} in-vivo.) The rate of incorporation of the TH{sup 3} was established. Concentrations between 0.0036 p mole x 10{sub 3} and 1.8 {mu}molex 10{sup -3} were tested. It was found that the per cent of cells incorporating the label is constant for the various concentrations of thymidine. The number of grains per nucleus increased with the increase of the concentration of the TH{sup 3} administered. After an incubation period which lasted 120 min no labelled mitosis was found. Further, the incorporation pattern was the same whether the label was added at the beginning of the incubation or after 160 min. Addition of TH{sup 3} to the same suspension at various time intervals did not alter the per cent of labelled cells but the number of grains per nucleus rose after every administration of the TH{sup 3}. Not all the cells in the suspension incorporated the same amount of TH{sup 3}. Quantitative measurements were made including grain counts, and the results showed that there is probably a deficiency of thymidine available to the leukaemic cells in their ascitic fluid. The dichotomy between the synthesis of the full complement of DNA and subsequent division of the cell will be discussed. (author) [French] En Israeel, le tritium a ete utilise comme indicateur isotopique pour l'etude de divers problemes biologiques. Les auteurs rendent compte des resultats d'experiences dans lesquelles ils ont employe la thymidine tritiee pour suivre la dynamique cellulaire dans la leucemie myeloiede experimentale ainsi que pour explorer le mecanisme de son incorporation a l'ADN des cellules leucemiques et des cellules eosinophiles de tumeurs ascitiques. Les cellules leucemiques ont ete marquees in vivo chez des rats par injection de thymidine tritiee dans la veine jugulaire. La dose etait de 1 {mu}c/g. Les auteurs ont evalue la vitesse de l'apparition des cellules marquees dans le sang peripherique et dans la tumeur ascitique. Au cours d'autres experiences, ils ont determine la vitesse de dilution de l'indicateur dans les noyaux, ce qui a permis d'evaluer le temps de doublement cellulaire dans la population de myelocites. Les auteurs ont etudie la dynamique des cellules leucemiques transfusees en injectant des myelocites marques dans la veine jugulaire de rats normaux et de rats leucemiques. Ils ont mesure la rapidite avec laquelle ces myelocites disparaissaient du sang. Ils ont cherche a determiner la presence de cellules marquees dans differents organes et constate que, peu de temps apres l'injection, ces cellules sont surtout capturees par les poumons, puis par la rate et, dans une moindre mesure, par le foie. Apres 24 heures, il n'etait plus possible de deceler de cellules marquees dans aucun des organes. On a ainsi obtenu des indications sur le sort de myelocites leucemiques dans differents organes, chez l'animal normal et chez l'animal leucemique. Au cours d'experiences in vitro, les auteurs ont ajoute a une 'suspension cellulaire' de la thymidine tritiee d'une concentration de 1 {mu}c/cm{sup 3}. Ils ont observe qu'au cours du marquage in vitro le nombre de cellules marquees etait 40 fois superieur a celui des mitoses. (La meme observation a ete faite apres adminisration de thymidine tritiee in vivo.) Les auteurs ont determine le taux d'incorporation de la thymidine tritiee. Us ont fait des essais pour des concentrations comprises entre 0,0036 {mu} mole x 10{sup -3} et 1,8 {mu}mole x 10{sup -3} et ont constate que le pourcentage des cellules qui fixent l'indicateur est constant quelle que soit la concentration. Le nombre de grains par noyau augmentait en meme temps que la concentration de la thymidine administree. Apres une periode d'incubation de 120 minutes, on n'a plus constate de mitose de cellulose marquees. De plus, le processus d'incorporation a ete le meme, que l'indicateur ait ete ajoute au debut de la periode d'incubation ou apres 160 minutes. L'addition, a differents intervalles de temps, n'a pas modifie le pourcentage des cellules marquees, mais le nombre de grains par noyau a augmente apres chaque administration de thymidine tritiee. Les cellules en suspension n'ont pas toutes fixe la meme quantite de thymidine tritiee. Les auteurs ont procede a des mesures quantitatives, notamment a des comptages de grains, et ont constate que les cellules leucemiques ne disposent probablement pas d'une quantite suffisante de thymidine dans le liquide ascitique. Les auteurs etudient la dichotomie entre la synthese du complement entier de l'ADN et la division subsequente de la cellule. (author) [Spanish] El tritio se utiliza en Israel como indicador para estudiar una gran variedad de problemas biologicos. Los autores describen en particular los resultados de unas investigaciones en que se ha utilizado timidina tritiada para estudiar la dinamica celular en la leucemia mieloide, inducida con lines experimentales, y analizar el mecanismo de la incorporacion de esa sustancia en el acido desoxirribonucleico de celulas leucemicas y de celulas de tumores asciticos de Ehrlich. Las celulas leucemicas se marcaron in vivo inyectando en la vena yugular de ratas timidina tritiada a razon de 1 {mu}curie/g de peso corporal. Se evaluo la velocidad de aparicion de las celulas marcadas en la sangre de la periferia y en el tumor ascitico de los animales. En otros experimentos se determino la velocidad de dilucion del indicador en los nucleos, lo que permitio calcular el tiempo de duplicacion de la poblacion de mielocitos. La dinamica de las celulas transfundidas se investigo inyectando mielocitos marcados en la vena yugular de ratas normales y leucemicas. Se midio su velocidad de desaparicion de la sangre. Se examinaron varios organos para ver si contenian celulas marcadas y se observo que poco despues de la inyeccion las celulas son principalmente captadas por los pulmones, despues por el bazo y, en menor grado, por el higado. Pasadas 24 h no se pudieron detectar celulas marcadas en ningun organo. De este modo, se obtuvo informacion sobre el comportamiento de los mielocitos leucemicos en distintos organos de animales normales y leucemicos. En experimentos realizados in vitro, se anadio a una suspension celular timidina tritiada en una concentracion de 1 {mu}curie/ml. Durante la marcacion in vitro se comprobo que el numero de celulas marcadas es 40 veces mayor que el de celulas en mitosis. El mismo fenomeno se observo despues de administrar in vivo timidina tritiada. Se calculo la velocidad de incorporacion de la timidina tritiada. Los experimentos se realizaron con concentraciones comprendidas entre 0,0036 ({mu} mol x 10{sup -3} y 1,8 {mu} mol x 10{sup -3}. Se observo que el porcentaje de celulas que incorporan la sustancia marcada es constante para las diferentes concentraciones de timidina. El numero de granulos por nucleo crece con la concentracion de la timidina tritiada. Despues de un periodo de incubacion de 120 min no se observaron celulas marcadas en mitosis. El regimen de incorporacion fue el mismo anadiendo la sustancia marcada al principio de la incubacion o al cabo de 160 min. La adicion de timidina tritiada a la misma suspension, a diferentes intervalos de tiempo, no altero el porcentaje de celulas marcadas, pero el numero de granulos por nucleo aumento despues de cada adicion. No todas las celulas presentes en la suspension incorporaron la misma cantidad de timidina tritiada. Se efectuaron mediciones cuantitativas, incluidos recuentos de granulos y los resultados demostraron que probablemente existe una insuficiencia de timidina en el fluido ascitico de las celulas leucemicas. Los autores estudian la dicotomia existente entre las sintesis del complemento del acido desoxirribonucleico y la division celular subsiguiente. (author) [Russian] Pri izuchenii razlichnyh biologicheskih problem v Izraile v kachestve izotopnogo indikatora ispol'zovalsja tritij. Byli opublikovany nekotorye rezul'taty, dlja polu- chenija kotoryh ispol'zovalsja tritirovannyj timidin (TN{sup 3}) s cel'ju na{sup l}juden- ijaza dinamikoj kletok jeksperimental'noj mielojdnoj lejkemii, a takzhe dlja izuchenija mehanizma ee vkljuchenija v DNK dlja podobnyh kletok i dlja ascitnyh opuholevyh kletok Jerliha. Lejkemichnye kletki metilis' v estestvennyh uslovijah putem vvedenija TN{sup 3} v shejnuju venu. Doza sostavljala 1 mkkjuri/g/na krysu. Byla proizvedena ocen- ka skorosti pojavlenija mechenyh kletok v periferijnoj krovi, a takzhe v ascit- noj opuholi zhivotnogo. Pri drugih jeksperimentah delalas' ocenka skorosti raz- bavlenija jetogo metjashhego veshhestva v jadrah, chto pozvolilo takim obrazom opredelit' vremja udvoenija kletok v mielocitnoj populjacii. Izuchalas' dinamika lejkemichnyh kletok posle perelivanija krovi putem vvedenija mechenogo mielocita v shejnuju venu obychnyh i stradajushhih lejkemiej krys. Byla izmerena skorost' ih ischeznovenija iz krovi. Byli izucheny razlichnye organy dlja mechenyh kletok, prichem najdeno, chto vskore posle vvedenija jetih kletok oni sosredotachivalis' glavnym obrazom v legkih, a pozdnee v selezenke i v ves'ma neznachitel'nom kolichestve v pecheni. Spustja 24 chasa ni v odnom organe ne bylo obnaruzheno nikakih mechenyh kletok. Takim obrazom byli polucheny svedenija o sud'be lejkemichnogo mielocita v razlichnyh organah normal'nyh i zarazhennyh lejkemiej zhivotnyh. Pri provedenii laboratornyh jeksperimentov TN{sup 3} dobavljalsja v suspenziju kletok v koncentracii 1 mkkmri/ml. V processe mechenija v la'oratornyh chslo- vijah bylo otmecheno, chto chislo mechenyh kletok bylo v 40 raz bol'she chisla mitozov. (To zhe samoe bylo obnaruzheno pri vvedenii TN{sup 3} v la'oratornyh uslovijah. 1 Byla ustanovlena skorost' vvedenija TN{sup 3}. Byli ispytany koncentracii ot 0,0036 mkmolbh 10{sup -3} do 1,8 mkmolbh Ju{sup -3} . Bylo ustanovleno, chto procent kletok, usvaivajushhih jeto metjashhee veshhestvo, javljaetsja postojannym dlja razlichnyh kon- centracij timidina. Chislo zeren na jadro uvelichivalos' po mere vozrastanija vvodimoj koncentracii TN{sup 3}. Posle okonchanija skrytogo perioda, prodolzhav- shegosja 120 minut, ne bylo obnaruzheno nikakih mechenyh mitozov. Dalee metod vvedenija metjashhego veshhestva.byl odinakovym kak pri dobavlenii ego v nachale skrytogo perioda, tak i posle 160 minut. Dobavlenie TN{sup 3} k toj zhe suspenzii' pri razlichnyh intervalah ne izmenilo procena mechenyh kletok, odnako chislo kletok na jadro vozrastalo posle kazhdogo vvedenija TN{sup 3}. Ne vse kletki v suspenzii usvoili odinakovoe kolichestvo TN{sup 3}. Byli provedeny kolichestvennye izmerenija, vkljuchaja podschet zeren, i rezul'- taty ukazali na vozmozhnoe nalichie nedostatochnosti timidina dlja lejkemi- chnyh kletok pri ih ascitnom techenii. Budet rassmotrena dihotomija mezhdu sintezom polnogo dobavlenija DNK i posledujushhim razdeleniem kletki. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}