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Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;

Conference:

Abstract

The advantages of the use of tritium-labelled compounds in radioautographic technique are discussed. Tritium electrons have a maximal energy of 0.018 MeV, corresponding to about 1{mu}m range in a photographic emulsion, and consequently they allow the highest possible resolution at a cellular and subcellular level. This is particularly useful for studying metabolic phenomena of tissues which are composed, as in the case of bone marrow, of different cellular types at various stages of differentiation. This technique has been used for investigating nucleic acids and protein metabolism of normal and leukaemic bone marrow cells. DNA metabolism has been studied utilizing a specific precursor, H{sup 3}-thymidine. Some significant differences of the percentages of labelled cells have been detected by comparing the normal and leukaemic elements belonging to the same stage of maturation. In acute leukaemia cells, particularly, a strikingly lower incorporation of thymidine was found and these results have been taken as evidence of a decreased proliferative capacity of these cells, as compared to normal myeloblasts. With the same technique, RNA and protein metabolism have been investigated utilizing H{sup 3}- uridine, H{sup 3}-leucine and H{sup 3}-phenylalanine as precursors. The existence of a strict interrelationship between RNA and protein metabolism is now fully  More>>
Authors:
Gavosto, F. [1] 
  1. Istituto di Clinica Medica Generale e Centro di Studi Fisico-Biologici, Universita de Turin, Turin (Italy)
Publication Date:
Feb 15, 1962
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences, Vienna (Austria), 3-10 May 1961; Other Information: 15 refs., 2 figs., 3 tabs.; Related Information: In: Tritium in the Physical and Biological Sciences. Vol. II. Proceedings of the Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences| 456 p.
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; BONE MARROW CELLS; DNA; LABELLED COMPOUNDS; LEUCINE; LEUKEMIA; METABOLISM; PHENYLALANINE; PHOTOGRAPHIC EMULSIONS; PRECURSOR; PROTEINS; RNA; THYMIDINE; TRACER TECHNIQUES; TRITIUM; TRITIUM COMPOUNDS; URIDINE
OSTI ID:
22190102
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3705014587
Submitting Site:
INIS
Size:
page(s) 237-245
Announcement Date:
Feb 14, 2014

Conference:

Citation Formats

Gavosto, F. Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;. IAEA: N. p., 1962. Web.
Gavosto, F. Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;. IAEA.
Gavosto, F. 1962. "Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;." IAEA.
@misc{etde_22190102,
title = {Nucleic Acids and Protein Metabolism of Bone Marrow Cells Studied by Means of Tritiumlabelled Precursors; Etude du Metabolisme des Acides Nucleiques et des Proteines dans les Cellules de la Moelle Osseuse, a l'Aide de Precurseurs Trities; 0418 0437 0443 0414 ; Estudio con Ayuda de Precursores Tritiados del Metabolismo de los acidos Nucleicos y de las Proteinas en las Celulas de la Medula Osea;}
author = {Gavosto, F.}
abstractNote = {The advantages of the use of tritium-labelled compounds in radioautographic technique are discussed. Tritium electrons have a maximal energy of 0.018 MeV, corresponding to about 1{mu}m range in a photographic emulsion, and consequently they allow the highest possible resolution at a cellular and subcellular level. This is particularly useful for studying metabolic phenomena of tissues which are composed, as in the case of bone marrow, of different cellular types at various stages of differentiation. This technique has been used for investigating nucleic acids and protein metabolism of normal and leukaemic bone marrow cells. DNA metabolism has been studied utilizing a specific precursor, H{sup 3}-thymidine. Some significant differences of the percentages of labelled cells have been detected by comparing the normal and leukaemic elements belonging to the same stage of maturation. In acute leukaemia cells, particularly, a strikingly lower incorporation of thymidine was found and these results have been taken as evidence of a decreased proliferative capacity of these cells, as compared to normal myeloblasts. With the same technique, RNA and protein metabolism have been investigated utilizing H{sup 3}- uridine, H{sup 3}-leucine and H{sup 3}-phenylalanine as precursors. The existence of a strict interrelationship between RNA and protein metabolism is now fully accepted in cellular biology. The existence of a constant ratio between uridine and amino acids incorporation has also been demonstrated in normal bone marrow cells. In acute leukaemia cells the incorporation of RNA and protein precursors, although different from case to case, is constantly and significantly lower. Furthermore, the ratio between uridine and amino acids incorporation is constantly altered in these cells. The lower RNA and protein metabolism and its dissociation in acute leukaemia cells is discussed in relation to the well-known maturation defect of these cells. (author) [French] L'auteur examine les avantages que presente l'emploi de composes trities pour l'autoradiographie. En effet les electrons du tritium ont une energie maximum de 0,018 MeV, correspondant a un parcours d'environ 1 micron dans une emulsion photographique, ce qui permet d'obtenir le plus fort degre de resolution possible aux niveaux cellulaire et infracellulaire. Ce phenomene est particulierement utile pour l'etude du metabolisme de tissus comme la moelle osseuse, qui sont composes de differents types de cellules a divers stades de differenciation. Cette technique a ete utilisee pour l'etude du metabolisme des acides nucleiques et des proteines des cellules normales et leucemiques de la moelle osseuse. L'auteur a etudie le metabolisme de l'ADN a l'aide d'un precurseur specifique, la thymidine tritiee. Il a decele certaines differences importantes dans les pourcentages de cellules marquees, en comparant les elements normaux et leucemiques au meme stade de maturation. Il a constate, en particulier, que dans les cellules d'un sujet atteint de leucemie aiguee, le taux d'incorporation de la thymidine etait beaucoup plus faible, ce qui prouve une diminution de la capacite de proliferation de ces cellules par rapport aux myeloblastes normaux. L'auteur a utilise la meme technique pour etudier le metabolisme de l'ARN et des proteines, a l'aide des precurseurs suivants: uridine, leucine et phenylalanine tritiees. Il est maintenant admis en biologie cellulaire qu'il existe un rapport etroit entre le metabolisme de l'ARN et celui des proteines; l'auteur a demontre qu'il existait egalement un rapport constant entre l'incorporation de l'uridine et des acides amines, dans les cellules normales de la moelle osseuse. Dans les cellules de sujets atteints de leucemie aiguee, l'incorporation de l'ARN et des precurseurs protidiques, bien qu'elle differe dans chaque cas, est toujours nettement plus faible. De plus, le rapport entre l'incorporation de l'uridine et celle des amino-acides varie constamment dans ces cellules. L'auteur examine ensuite la diminution et la dissociation du metabolisme de l'ARN et des proteines, dans les cellules de sujets atteints de leucemie aiguee, par rapport aux anomalies bien connues que presente le processus de maturation de ces cellules. (author) [Spanish] El autor examina las ventajas que presenta el empleo de los compuestos marcados con tritio en la tecnica autorradiografica. En efecto, los electrones emitidos por el tritio tienen una energia maxima de 0,018 MeV, que corresponde, aproximadamente, a un alcance de 1 miera en una emulsion fotografica y permiten, por lo tanto, lograr el maximo poder de resolucion al nivel celular y subcelular. Ello es particularmente util para estudiar fenomenos metabolicos en tejidos que se componen, como en el caso de la medula osea, de celulas de varios tipos en diversos estados de diferenciacion. Esta tecnica se ha aplicado al estudio del metabolismo de los acidos nucleicos y de las proteinas en las celulas normales y leucemicas de la medula osea. El metabolismo del acido desoxirribonucleico se ha estudiado utilizando un precursor especifico, la timidina-{sup 3}H. Comparando los elementos normales y leucemicos que han alcanzado un grado de madurez analogo, se han observado diferencias significativas en el porcentaje de celulas marcadas. Especialmente, en los casos de leucemia aguda, se ha comprobado que la incorporacion de timidina en las celulas leucemicas disminuye muy notablemente. Se estima que estos resultados constituyen una prueba de que la capacidad de proliferacion de estas celulas disminuye en comparacion con la de los mieloblastos normales. Por este mismo procedimiento, se ha estudiado el metabolismo del acido ribonucleico y de las proteinas, utilizando como precursores uridina-{sup 3}H, leucina-{sup 3}H y fenilalanina-{sup 3}H. Actualmente, en biologia celular se admite sin reservas la existencia de una estrecha relacion entre el metabolismo del acido ribonucleico y el de las proteinas. Ademas, se ha demostrado que en las celulas normales de la medula osea la razon entre la incorporacion de uridina y la de aminoacidos permanece constante. En los casos de leucemia aguda, la incorporacion de acido ribonucleico y de precursores proteinicos en las celulas leucemicas es siempre sensiblemente mas reducida, aunque varia de un caso a otro. Asimismo, la razon entre la incorporacion de uridina y la de aminoacidos se altera constantemente en estas celulas. El autor estudia la disminucion y disociacion del metabolismo del acido ribonucleico y de las proteinas en las celulas leucemicas (leucemia aguda), relacionando estos fenomenos con la maduracion defectuosa, tipica de estas celulas. (author) [Russian] Obsuzhdajutsja prei- mushhestva ispol'zovanija mechennyh tritiem slozhnyh soedinenij v metodah avto- radiograficheskogo analiza. Dejstvitel'no, jelektrony tritija obladajut maksi- mal'noj jenergiej 0,018 Mjev, chto sootvetstvuet priblizitel'no rasstojaniju 1 mikrona na fotograficheskoj plenke, dostigaja tem samym nailuchshej vozmozh- noj razreshajushhej sposobnosti v masshtabe kletok i ih komponentov. Jeto osobenno polezno pri izuchenii javlenij metabolizma v tkanjah, sostojashhih; kak jeto imeet mestno v kostnom mozge, iz raznovidnostej kletok, nahodjashhihsja na razlichnoj stadii differenciacii. Jetot metod byl ispol'zovan dlja issledovanija metabolizma nukleinovyh kislot i proteina v zdorovyh i porazhennyh lejkemiej kletkah kostnogo mozga. Izuchenie metabolizma dioksiribonukleinovoj kisloty proizvodilos' pri pomoshhi specificheskogo predshestvennika, a imenno timidina tritija. Putem sopostavlenija nahodjashhihsja na odnoj i toj zhe stadii sozrevanija chastic zdoro- vyh i porazhennyh lejkemiej tkanej byli obnaruzheny znachitel'nye rashozhde- nija v procentnyh doljah mechenyh kletok. V chastnosti, v jachejkah, porazhennyh ostroj lejkemiej, nabnjudalos' gorazdo bolee slaboe pogloshhenie timidina, chto prinimalos' za dokazatel'stvo men'shej sposobnosti razmnozhenija jetih kletochek po sravneniju s normal'nymi mieloblastami. Po tomu zhe samomu metodu byl izuchen metabolizm ribonukleinovoj kisloty i proteina, dlja chego v kachestve predshestvennikov byli ispol'zovany uridin, lejcin i fenil-alanin tritija. V nastojashhee vremja v kletochnoj biologii s nesomnennost'ju dopuskaetsja nali- chie tochnogo sootnoshenija mezhdu metabolizmom ribonukleinovoj kisloty i proteina. Bylo takzhe dokazano nalichie postojannogo sootnoshenija mezhdu poglo- shheniem uridina i aminokislot zdorovymi kletkami kostnogo mozga. V kletkah, porazhennyh ostroj lejkemiej, pogloshhenie predshestvennikov ribonukleinovoj kisloty i proteina, hotja ono i menjaetsja ot sluchaja k sluchaju, neizmenno prois- hodit v znachitel'no men'shej dole. Bolee togo, v jetih kletkah sootnoshenie mezhdu pogloshheniem uridina i aminokislot postojanno menjaetsja. Obsuzhdaetsja oslablenie metabolizma ribonukleinovoj kisloty i proteina i razlozhenie ih v kletkah, porazhennyh ostroj lejkemiej, chto sopostavljaetsja s horosho izvestnym nedostatkom sozrevaemosti jetih kletok. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}