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The Inactivation of Escherichia Coli Bacteria Labelled with Tritiated Thymidine; Inactivation de Bacteries Escherichia Coli Marquees par la Thymidine Tritiee; 0418 043d 0414 ; Inactivacion de Escherichia Coli Marcada con Timidina Tritiada

Conference:

Abstract

Bacteria of the strain B{sup 3}{sub 1} thy {sup -}/Sr, which required thymine and are streptomycin-resistant, had their DNA labelled with tritiated thymidine. The radioactivity measurements were made with a liquid scintillation counting system, with two photomultipliers mounted in coincidence. Under these conditions, the efficiency of the measures was 4.5% and the background 130 counts/min. The radioactive bacteria were kept in sealed tubes either at 0 Degree-Sign C or at - 196 Degree-Sign C and their survival studied. These experiments showed that the radioactive bacteria are inactivated exponentially as a function of the number of tritium atoms disintegrated. The inactivation is temperature dependent. In both cases the killing efficiency per nuclear transmutation was determined and found as very low. The number of ion pairs generated by the {beta}-particles emitted as a consequence of the transmutation of H{sup 3} was evaluated and found quite comparable with the one found in the case of X-rays. The suicide caused by the H{sup 3} disintegrations seems to be directly linked with the ionizations produced by the {beta}-particles inside the bacterial DNA. (author) [French] Des bacteries de la souche B{sup 3}{sub 1} thy {sup -}/Sr, exigeantes en thymine et resistantes a la streptomycine, ont ete  More>>
Authors:
Apelgot, Sonia [1] 
  1. Institut du Radium, Laboratoire Curie, Paris (France)
Publication Date:
Feb 15, 1962
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences, Vienna (Austria), 3-10 May 1961; Other Information: 12 refs., 5 figs., 3 tabs.; Related Information: In: Tritium in the Physical and Biological Sciences. Vol. II. Proceedings of the Symposium on the Detection and Use of Tritium in the Physical and Biological Sciences| 456 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY; BIOLOGICAL RADIATION EFFECTS; DNA; ESCHERICHIA COLI; INACTIVATION; PHOTOMULTIPLIERS; RADIOACTIVITY; SCINTILLATION COUNTING; STREPTOMYCIN; THYMIDINE; THYMINE; TRANSMUTATION; TRITIUM; X RADIATION
OSTI ID:
22190097
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
French
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3699014582
Submitting Site:
INIS
Size:
page(s) 167-178
Announcement Date:
Feb 14, 2014

Conference:

Citation Formats

Apelgot, Sonia. The Inactivation of Escherichia Coli Bacteria Labelled with Tritiated Thymidine; Inactivation de Bacteries Escherichia Coli Marquees par la Thymidine Tritiee; 0418 043d 0414 ; Inactivacion de Escherichia Coli Marcada con Timidina Tritiada. IAEA: N. p., 1962. Web.
Apelgot, Sonia. The Inactivation of Escherichia Coli Bacteria Labelled with Tritiated Thymidine; Inactivation de Bacteries Escherichia Coli Marquees par la Thymidine Tritiee; 0418 043d 0414 ; Inactivacion de Escherichia Coli Marcada con Timidina Tritiada. IAEA.
Apelgot, Sonia. 1962. "The Inactivation of Escherichia Coli Bacteria Labelled with Tritiated Thymidine; Inactivation de Bacteries Escherichia Coli Marquees par la Thymidine Tritiee; 0418 043d 0414 ; Inactivacion de Escherichia Coli Marcada con Timidina Tritiada." IAEA.
@misc{etde_22190097,
title = {The Inactivation of Escherichia Coli Bacteria Labelled with Tritiated Thymidine; Inactivation de Bacteries Escherichia Coli Marquees par la Thymidine Tritiee; 0418 043d 0414 ; Inactivacion de Escherichia Coli Marcada con Timidina Tritiada}
author = {Apelgot, Sonia}
abstractNote = {Bacteria of the strain B{sup 3}{sub 1} thy {sup -}/Sr, which required thymine and are streptomycin-resistant, had their DNA labelled with tritiated thymidine. The radioactivity measurements were made with a liquid scintillation counting system, with two photomultipliers mounted in coincidence. Under these conditions, the efficiency of the measures was 4.5% and the background 130 counts/min. The radioactive bacteria were kept in sealed tubes either at 0 Degree-Sign C or at - 196 Degree-Sign C and their survival studied. These experiments showed that the radioactive bacteria are inactivated exponentially as a function of the number of tritium atoms disintegrated. The inactivation is temperature dependent. In both cases the killing efficiency per nuclear transmutation was determined and found as very low. The number of ion pairs generated by the {beta}-particles emitted as a consequence of the transmutation of H{sup 3} was evaluated and found quite comparable with the one found in the case of X-rays. The suicide caused by the H{sup 3} disintegrations seems to be directly linked with the ionizations produced by the {beta}-particles inside the bacterial DNA. (author) [French] Des bacteries de la souche B{sup 3}{sub 1} thy {sup -}/Sr, exigeantes en thymine et resistantes a la streptomycine, ont ete marquees dans leur ADN par la thymidine tritiee. Les mesures de radioactivite ont ete faites avec un detecteur a scintillation en milieu liquide comprenant deux photomultiplicateurs montes en coiencidence. Dans nos conditions, l'efficacite des mesures a ete de 4,5% et le mouvement propre de 130 coups/min. Les bacteries radioactives ont ete conservees en ampoules scellees soit a 0 Degree-Sign C soit a - 196 Degree-Sign C, et la survie etudiee. Ces experiences ont montre que les bacteries sont inactivees exponentiellement en fonction du nombre d'atomes de tritium desintegres. L'inactivation depend de la temperature a laquelle elles sont conservees. Le calcul montre que l'efficacite de chaque atome desintegre pour tuer une bacterie est tres faible. Le calcul du nombre de paires d'ions crees, a l'inactivation, dans la bacterie par les particules {beta} emises au cours de la transmutation des atomes de {sup 3}H montre que ce nombre est comparable a celui laisse dans le cas des rayons X. Il semble que le suicide provoque par les desintegrations de {sup 3}H soit lie directement, a - 196 Degree-Sign C, a l'ionisation produite, dans l'ADN bacterien, par des particules {beta} emises, et a 0 Degree-Sign C, a la transmutation des atomes de {sup 3}H elle-meme. (author) [Spanish] tritiada. Se ha marcado con timidina tritiada el acido desoxirribonucleico de bacterias de la cepa B; thy {sup -}/Sr, que requieren timidina y son resistentes a la estreptomicina. Las mediciones de radiactividad se efectuaron con un contador de centelleador liquido, provisto de dos fotomultiplicadores montados en coincidencia. En las condiciones experimentales utilizadas, el rendimiento de recuento fue del 4,5% y la actividad de fondo, de 130 impulsos por minuto. Las bacterias radiactivas se guardaron a 0 Degree-Sign C y a - 196 Degree-Sign C en ampollas soldadas y se estudio su supervivencia. Los experimentos demuestran que las bacterias radiactivas son inactivadas exponencialmente, en funcion del numero de atomos de tritio que se desintegran. La inactivacion depende tambien de la temperatura. En ambos casos, se determino el efecto letal por transmutacion nuclear comprobandose que es muy debil. Se calculo el numero de pares ionicos generados por las particulas beta emitidas a consecuencia de la transmutacion del {sup 3}H y se observo que es comparable al obtenido en el caso de los rayos X. La muerte, causada por las desintegraciones del {sup 3}H, parece estar directamente relacionada con a ionizacion producida por las particulas beta en el acido desoxirribonucleico de las bacterias. (author) [Russian] Bakterii shtamma B{sup 3}{sub 1} thy{sup -}/Sr, nuzhdajushhiesja v timine i stojkie k strepto- micinu, byli markirovany v ih DNK timidinom, mechennym tritiem. Izmerenie radioaktivnosti bylo sdelano pri pomoshhi zhidkogo scintilljacionnogo detek- tora s dvumja fotoumnozhiteljami sovpadenij. V dannyh uslovijah jeffektivnost' izmerenij ravnjalas' 5,5%, a fon - 130 kjuri/mol'. Radioaktivnye bakterii sohranjalis' v zapechatnanyh ampulah libo pri tem- perature 0 Degree-Sign C, libo pri temperature - 196 Degree-Sign C, i izuchalos' ih dal'nejshee sosto- janie. Jeti opyty pokazali, chto bakterii inaktivirujutsja funkcional'no v zavisi- mosti ot chisla rasshheplennyh atomov tritija. Inaktivacija zavisit ot tem- peratzgry, v kotoroj oni soderzhalis'. Vychislenie pokazyvaet, chto zffektiv nost' kazhdogo rasshheplennogo atoma dlja umershhvlenija bakterij ochen' neznachi- tel'na. Vychislenie kolichestva par sozdannyh pri inaktivacii ionov v bak- terii ispushhennymi beta-chasticami, poluchennymi vo vremja transmutacii atomov tritija, pokazyvaet, chto ono sravnimo s kolichestvom v sluchae s iks-luchami. Kazhetsja, chto vymiranie, vyzvannoe raspadom trutija, dolzhno byt' prjamo svjazano s proizvedinnoj ionzaciej v bakterial'noj DNK ispushhennymi beta- chasticami. (author)}
place = {IAEA}
year = {1962}
month = {Feb}
}