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Cytogenetics of Post-Irradiation Mouse Leukaemia

Abstract

The interrelationship between radiation, cytogenetic abnormalities, and viruses in leukaemogenesis has been studied in the RF/Un mouse which develops a high incidence of granulocytic leukaemia on radiation exposure. A virus-like agent has been demonstrated in such leukaemic animals and the disease has been transmitted by passage of apparently acellular materials from irradiated primary animals to normal recipients. Pilot cytogenetic studies revealed consistent abnormal chromosome markers and modal shifts in both irradiated leukaemic animals and in non-irradiated animals developing leukaemia after passage injection. To define better the relationship between consistent bone-marrow chromosome aberrations and postirradiation primary and passaged leukaemia, 100 RF/Un mice were studied which were irradiated with 300 R of 250-kVp X-rays at 100 weeks of age and subsequently developed leukaemia. Eighty-seven had granulocytic leukaemia and in 72 of these, bone-marrow cytogenetic abnormalities were found. The distribution of-numerical and structural chromosome aberrations in 3225 cells studied are reviewed in derail. The correlation of specific aberrations to clinical and histopathologic findings has been attempted: Sequential passages of apparently cell-free material from the post-irradiation leukaemic mice into unirradiated RE/Un recipients and subsequent passages from leukaemic recipients were performed to observe the evolution of any initial chromosome markers and shifts in modal chromosome  More>>
Authors:
Wald, N.; Pan, S.; Upton, A.; Brown, R.; [1]  Oak Ridge National Laboratory, Oak Ridge, TN (United States)]
  1. Graduate School of Public Health, University of Pittsburgh, PA (United States)
Publication Date:
Nov 15, 1969
Product Type:
Conference
Report Number:
IAEA-SM-118/1
Resource Relation:
Conference: Symposium on Radiation-Induced Cancer, Athens (Greece), 28 Apr - 2 May 1969; Other Information: 49 refs., 3 figs., 8 tabs.; Related Information: In: Radiation-Induced Cancer. Proceedings of a Symposium on Radiation-Induced Cancer| 512 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BONE MARROW; CHROMOSOMAL ABERRATIONS; CHROMOSOMES; IRRADIATION; LEUKEMIA; MICE; VIRUSES
OSTI ID:
22182158
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); World Health Organization, Geneva (Switzerland)
Country of Origin:
IAEA
Language:
English
Contract Number:
Grant CA08466; Contract NASr-169
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3973006657
Submitting Site:
INIS
Size:
page(s) 249-264
Announcement Date:
Jan 23, 2014

Citation Formats

Wald, N., Pan, S., Upton, A., Brown, R., and Oak Ridge National Laboratory, Oak Ridge, TN (United States)]. Cytogenetics of Post-Irradiation Mouse Leukaemia. IAEA: N. p., 1969. Web.
Wald, N., Pan, S., Upton, A., Brown, R., & Oak Ridge National Laboratory, Oak Ridge, TN (United States)]. Cytogenetics of Post-Irradiation Mouse Leukaemia. IAEA.
Wald, N., Pan, S., Upton, A., Brown, R., and Oak Ridge National Laboratory, Oak Ridge, TN (United States)]. 1969. "Cytogenetics of Post-Irradiation Mouse Leukaemia." IAEA.
@misc{etde_22182158,
title = {Cytogenetics of Post-Irradiation Mouse Leukaemia}
author = {Wald, N., Pan, S., Upton, A., Brown, R., and Oak Ridge National Laboratory, Oak Ridge, TN (United States)]}
abstractNote = {The interrelationship between radiation, cytogenetic abnormalities, and viruses in leukaemogenesis has been studied in the RF/Un mouse which develops a high incidence of granulocytic leukaemia on radiation exposure. A virus-like agent has been demonstrated in such leukaemic animals and the disease has been transmitted by passage of apparently acellular materials from irradiated primary animals to normal recipients. Pilot cytogenetic studies revealed consistent abnormal chromosome markers and modal shifts in both irradiated leukaemic animals and in non-irradiated animals developing leukaemia after passage injection. To define better the relationship between consistent bone-marrow chromosome aberrations and postirradiation primary and passaged leukaemia, 100 RF/Un mice were studied which were irradiated with 300 R of 250-kVp X-rays at 100 weeks of age and subsequently developed leukaemia. Eighty-seven had granulocytic leukaemia and in 72 of these, bone-marrow cytogenetic abnormalities were found. The distribution of-numerical and structural chromosome aberrations in 3225 cells studied are reviewed in derail. The correlation of specific aberrations to clinical and histopathologic findings has been attempted: Sequential passages of apparently cell-free material from the post-irradiation leukaemic mice into unirradiated RE/Un recipients and subsequent passages from leukaemic recipients were performed to observe the evolution of any initial chromosome markers and shifts in modal chromosome number in the passage generations. Two-hundred-thirty-six mice were inoculated with the material obtained either from primary post-irradiation leukaemic mice or from serially-passaged leukaemia cases. In the most extensive passaged line, 22 transfer generations containing 129 leukaemic mice were examined by clinical, histopathologic, -haematologic and cytogenetic procedures. Evolution of abnormal chromosome modes from 41 in the early passages to 39 chromosomes consistently after the 4th transfer generation was observed. The relationship of these changes to the clinico-morphologic characteristics of the leukaemia is under analysis. Both positive and negative experimental evidence accumulating concerning the acellularity of the passaged material are considered. (author)}
place = {IAEA}
year = {1969}
month = {Nov}
}