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Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform

Abstract

The diversity of bacterial communities exposed to radioactive contamination in Chernobyl soils was examined by a combination of molecular and culture-based approaches. A set of six radioactive soil samples, exhibiting high levels of {sup 137}Cs contamination, were collected from the T22 trench. Three samples were also collected in nearby soils with low contamination. Complex bacterial community structures were observed in both highly and weakly contaminated samples, using a molecular approach targeting the 16S rRNA gene. However, the presence of specific populations within samples from highly contaminated soils could not be revealed by statistical analysis of the DGGE profiles. More than 200 culturable isolates, representative of dominant morphotypes, were grouped into 83 Operational Taxonomic Units (OTUs) and affiliated to Firmicutes, Actinobacteria, Alpha-, Beta-, Gamma-Proteobacteria and Bacteroiedetes. No specific pattern linked to contamination was observed for these culturable bacteria. The results show that both highly and weakly contaminated soils host a wide diversity of bacteria, suggesting that long term exposure to radionuclides does not lead to the extinction of bacterial diversity.
Authors:
Chapon, Virginie; [1]  CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France); Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)]; Piette, Laurie; [1]  CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France); Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)]; Vesvres, Marie-Helene; [2]  Coppin, Frederic; [3]  Marrec, Claire Le; [4]  Christen, Richard; [5]  CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)]; Theodorakopoulos, Nicolas; [1]  CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France); Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France); Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France); IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)]; Fevrier, Laureline; [3]  others, and
  1. CEA, DSV, IBEB, SBVME, LIPM, F-13108 Saint-Paul-lez-Durance (France)
  2. Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France)
  3. IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)
  4. ISVV, UMR 1219, Institut Polytechnique de Bordeaux/INRA, POB 50008, F-33882 Villenave d'Ornon (France)
  5. Universite de Nice-Sophia-Antipolis, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)
Publication Date:
Jul 15, 2012
Product Type:
Journal Article
Resource Relation:
Journal Name: Applied Geochemistry; Journal Volume: 27; Journal Issue: 7; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
54 ENVIRONMENTAL SCIENCES; 58 GEOSCIENCES; BACTERIA; CESIUM 137; CHERNOBYLSK-4 REACTOR; ENVIRONMENTAL IMPACTS; RADIOECOLOGICAL CONCENTRATION; REACTOR ACCIDENTS; SOILS
OSTI ID:
22152059
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0883-2927; CODEN: APPGEY; Other: PII: S0883-2927(11)00385-4; TRN: GB12R4107106193
Availability:
Available from http://dx.doi.org/10.1016/j.apgeochem.2011.08.011
Submitting Site:
INIS
Size:
page(s) 1375-1383
Announcement Date:
Nov 21, 2013

Citation Formats

Chapon, Virginie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Piette, Laurie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Vesvres, Marie-Helene, Coppin, Frederic, Marrec, Claire Le, Christen, Richard, CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)], Theodorakopoulos, Nicolas, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France), Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France), IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)], Fevrier, Laureline, and others, and. Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform. United Kingdom: N. p., 2012. Web. doi:10.1016/J.APGEOCHEM.2011.08.011.
Chapon, Virginie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Piette, Laurie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Vesvres, Marie-Helene, Coppin, Frederic, Marrec, Claire Le, Christen, Richard, CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)], Theodorakopoulos, Nicolas, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France), Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France), IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)], Fevrier, Laureline, & others, and. Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform. United Kingdom. doi:10.1016/J.APGEOCHEM.2011.08.011.
Chapon, Virginie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Piette, Laurie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Vesvres, Marie-Helene, Coppin, Frederic, Marrec, Claire Le, Christen, Richard, CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)], Theodorakopoulos, Nicolas, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France), Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France), IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)], Fevrier, Laureline, and others, and. 2012. "Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform." United Kingdom. doi:10.1016/J.APGEOCHEM.2011.08.011. https://www.osti.gov/servlets/purl/10.1016/J.APGEOCHEM.2011.08.011.
@misc{etde_22152059,
title = {Microbial diversity in contaminated soils along the T22 trench of the Chernobyl experimental platform}
author = {Chapon, Virginie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Piette, Laurie, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France)], Vesvres, Marie-Helene, Coppin, Frederic, Marrec, Claire Le, Christen, Richard, CNRS, UMR 6543, Centre de Biochimie, Parc Valrose, F-06108 Nice (France)], Theodorakopoulos, Nicolas, CNRS, UMR 6191, F-13108 Saint-Paul-lez-Durance (France), Universite d'Aix-Marseille, F-13108 Saint-Paul-lez-Durance (France), Universite de Bordeaux 1/CNRS-IN2P3, UMR 5797, CENBG, POB 120, F-33175 Gradignan cedex (France), IRSN, DEI/SECRE/LRE-Bat 186, B.P.3, Cadarache Center, F-13115 Saint-Paul-lez-Durance cedex (France)], Fevrier, Laureline, and others, and}
abstractNote = {The diversity of bacterial communities exposed to radioactive contamination in Chernobyl soils was examined by a combination of molecular and culture-based approaches. A set of six radioactive soil samples, exhibiting high levels of {sup 137}Cs contamination, were collected from the T22 trench. Three samples were also collected in nearby soils with low contamination. Complex bacterial community structures were observed in both highly and weakly contaminated samples, using a molecular approach targeting the 16S rRNA gene. However, the presence of specific populations within samples from highly contaminated soils could not be revealed by statistical analysis of the DGGE profiles. More than 200 culturable isolates, representative of dominant morphotypes, were grouped into 83 Operational Taxonomic Units (OTUs) and affiliated to Firmicutes, Actinobacteria, Alpha-, Beta-, Gamma-Proteobacteria and Bacteroiedetes. No specific pattern linked to contamination was observed for these culturable bacteria. The results show that both highly and weakly contaminated soils host a wide diversity of bacteria, suggesting that long term exposure to radionuclides does not lead to the extinction of bacterial diversity.}
doi = {10.1016/J.APGEOCHEM.2011.08.011}
journal = {Applied Geochemistry}
issue = {7}
volume = {27}
journal type = {AC}
place = {United Kingdom}
year = {2012}
month = {Jul}
}