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Pancreas Scanning with Se{sup 75}-Selenomethionine; Scintigraphie du Pancreas avec de la Selenomethionine Marquee au Selenium-75; Skennirovanie podzheludochnoj zhelezy s pomoshch'yu selenometionina, mechennogo Selenom-75; Exploracion del Pancreas con Selenometionina-{sup 75}Se

Conference:

Abstract

Amino-acids show a high degree of localization in the pancreas during the first few hours after administration. This specificity is related to the rapid synthesis and turnover of the digestive enzymes. Since none of the elements in the structure of amino-acids (C,H, N,0 and S) have suitable gamma-emitting isotopes, they cannot be used for scanning. Several I{sup 131} derivatives of amino-acids were prepared and tested, but the pancreas-localizing property of the original amino-acid was lost in every case. The chemical similarity of selenium and sulphur suggested that the selenium analogue of methionine might resemble methionine closely enough to take its place in protein synthesis. Se{sup 75} (halflife, 128 d; principal gamma-ray, 0.27 MeV) is suitable for scanning. Selenium-75-selenomethionine was prepared by yeast biosynthesis. The yeast was grown on a low sulphur medium containing high specific activity Se{sup 75} as selenite. After extraction with ether and hot trichloracetic acid, the protein residue was hydrolysed with hydrochloric acid The resulting amino-acid mixture was separated by column chromatography. Selenium-75-selenomethionine prepared in this manner showed biological properties of a natural aminoacid. The uptake in pancreas was about 7% of the administered dose and the concentration in a gramme of pancreas was seven times that  More>>
Authors:
Blau, M. [1] 
  1. Department of Nuclear Medicine, Roswell Park Memorial Institute, Buffalo, NY (United States)
Publication Date:
Oct 15, 1964
Product Type:
Conference
Report Number:
IAEA-SM-51/68
Resource Relation:
Conference: Symposium on medical radioisotope scanning, Athens (Greece), 20-24 Apr 1964; Other Information: Abstract also available in French; 12 refs., 7 figs., 3 tabs.; Related Information: In: Medical Radioisotope Scanning, Vol. II. Proceedings of the Symposium on Medical Radioisotope Scanning| 484 p.
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; BIOSYNTHESIS; BLOOD; CHROMATOGRAPHY; DIGESTIVE SYSTEM DISEASES; ENZYMES; EXTRACTION; GAMMA RADIATION; HALF-LIFE; HYDROCHLORIC ACID; IODINE 131; LIVER; METHIONINE; PANCREAS; RADIOISOTOPE SCANNING; SELENIUM; SELENIUM 75; SPECIFICITY; YEASTS
OSTI ID:
22143629
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3087098335
Submitting Site:
INIS
Size:
page(s) 275-287
Announcement Date:
Oct 31, 2013

Conference:

Citation Formats

Blau, M. Pancreas Scanning with Se{sup 75}-Selenomethionine; Scintigraphie du Pancreas avec de la Selenomethionine Marquee au Selenium-75; Skennirovanie podzheludochnoj zhelezy s pomoshch'yu selenometionina, mechennogo Selenom-75; Exploracion del Pancreas con Selenometionina-{sup 75}Se. IAEA: N. p., 1964. Web.
Blau, M. Pancreas Scanning with Se{sup 75}-Selenomethionine; Scintigraphie du Pancreas avec de la Selenomethionine Marquee au Selenium-75; Skennirovanie podzheludochnoj zhelezy s pomoshch'yu selenometionina, mechennogo Selenom-75; Exploracion del Pancreas con Selenometionina-{sup 75}Se. IAEA.
Blau, M. 1964. "Pancreas Scanning with Se{sup 75}-Selenomethionine; Scintigraphie du Pancreas avec de la Selenomethionine Marquee au Selenium-75; Skennirovanie podzheludochnoj zhelezy s pomoshch'yu selenometionina, mechennogo Selenom-75; Exploracion del Pancreas con Selenometionina-{sup 75}Se." IAEA.
@misc{etde_22143629,
title = {Pancreas Scanning with Se{sup 75}-Selenomethionine; Scintigraphie du Pancreas avec de la Selenomethionine Marquee au Selenium-75; Skennirovanie podzheludochnoj zhelezy s pomoshch'yu selenometionina, mechennogo Selenom-75; Exploracion del Pancreas con Selenometionina-{sup 75}Se}
author = {Blau, M.}
abstractNote = {Amino-acids show a high degree of localization in the pancreas during the first few hours after administration. This specificity is related to the rapid synthesis and turnover of the digestive enzymes. Since none of the elements in the structure of amino-acids (C,H, N,0 and S) have suitable gamma-emitting isotopes, they cannot be used for scanning. Several I{sup 131} derivatives of amino-acids were prepared and tested, but the pancreas-localizing property of the original amino-acid was lost in every case. The chemical similarity of selenium and sulphur suggested that the selenium analogue of methionine might resemble methionine closely enough to take its place in protein synthesis. Se{sup 75} (halflife, 128 d; principal gamma-ray, 0.27 MeV) is suitable for scanning. Selenium-75-selenomethionine was prepared by yeast biosynthesis. The yeast was grown on a low sulphur medium containing high specific activity Se{sup 75} as selenite. After extraction with ether and hot trichloracetic acid, the protein residue was hydrolysed with hydrochloric acid The resulting amino-acid mixture was separated by column chromatography. Selenium-75-selenomethionine prepared in this manner showed biological properties of a natural aminoacid. The uptake in pancreas was about 7% of the administered dose and the concentration in a gramme of pancreas was seven times that in a gramme of liver. Blood and other organs had even lower uptakes. The clinical usefulness of pancreas scanning is now being evaluated. Tumours of the pancreas have been found as areas of decreased uptake. There is no concentration of selenomethionine in cases of acute pancreatitis. It is too early to give a statistical estimate of our ability to find tumours of the pancreas by this technique. The estimated total body dose from this procedure is less than 1 rad. The selenium dose is only a. few microgrammes and represents no toxicity hazard. (author) [Spanish] Los aminoacidos tienden a localizarse en el pancreas durante las primeras horas que siguen a su administracion. Esta especificidad esta relacionada con la rapidez de la sintesis y de la renovacion de las enzimas digestivas. Como ninguno de los elementos que componen los aminoacidos (C, H, N, O y S) tiene isotopos emisores de rayos gamma apropiados, no es posible recurrir a ellos para la exploracion centelleografica. Los autores prepararon y ensayaron varios derivados de aminoacidos marcados con 131I, pero en todos los casos comprobaron la desaparicion de la propiedad de localizarse en el pancreas caracteristica del aminoacido original. La semejanza qufmica entre el selenio y el azufre sugirio a los autores la posibilidad de que el analogo seleniado de la metionina se pareciese a esta lo suficiente para poder sustituirla en la sintesis proteica. El selenio-75 (periodo: 128 d; rayos gamma principales: 0,27 MeV) es adecuado para la exploracion. La selenometionina-75Se fue preparada biosinteticamente mediante levaduras. Estas levaduras se cultivaron en un medio con poco azufre que contenia de alta actividad especifica, en forma de selenito. El residuo protefnico extraido con eter y acido tricloracetico en caliente, se hidrolizo con acido clorhidrico. La mezcla de aminoacidos obtenida se separo por cromatografia en columna. La selenometionina-{sup 75} asi preparada tiene las propiedades biologicas de un aminoacido natural. El pancreas absorbio el 7%, aproximadamente, de la dosis administrada y la concentracion por gramo de tejido fue siete veces mayor en el pancreas que en el higado. Menor aun que en este fue la concentracion en la sangre y en otros Organos. Los autores estan ahora evaluando la utilidad clinica de la exploracion del pancreas. Han comprobado que los tumores del pancreas constituyen zonas de absorcion disminuida. En los casos de pancreatitis aguda no se produce concentracion de selenometionina. Seria prematuro estimar estadisticamente la posibilidad de aplicar esta tecnica para descubrir tumores del pancreas. Se estima que la dosis corporal total imputable a la aplicacion de este procedimiento es inferior a 1 rad. La dosis de selenio es de algunos microgramos solamente y no entrana ningun riesgo de toxicidad. (author) [Russian] Obnaruzhena vysokaja stepen' koncentracii aminokislot v podzheludochnoj zheleze v techenie pervyh neskol'kih chasov posle vvedenija. Jeta osobennost' svjazana s bystrym sintezom i krugooborotom pishhevaritel'nyh fermentov. Poskol'ku ni odin iz jelementov v strukture aminokislot (C,H,N i S) ne imeet sootvetstvujushhih gamma- izluchajushhih izotopov, ih nel'zja ispol'zovat' dlja skennirovanija. Byli podgotovleny i ispytany neskol'ko proizvodnyh aminokislot -jod-131, no v kazhdom sluchae terjalos' svojstvo ishodnoj aminokisloty sosredotochivat'sja v podzheludochnoj zheleze. Himicheskoe shodstvo selena i sery daet osnovanie schitat', chto selenovyj analog meteonina blizok k metioninu po stroeniju v dostatochnoj stepeni, chtoby zanjat' ego mesto pri sinteze belka. Selen-75 (period poluraspada 128 dnej, osnovnye dannye: gamma-luchi, 0,27 Mjev) prigoden dlja skennirovanija. Prigotavlivalsja selenometionin - selen-75 s pomoshh'ju biosinteza drozhzhej. Drozhzhi vyrashhivali na srede s nizkim soderzhaniem sery i vysokoj udel'noj aktivnost'ju selena-75 v vide selenita. Posle jekstragirovanija jefirom i gorjachej trihloruksusnoj kislotoj belkovyj ostatok podvergali gidrolizu s pomoshh'ju soljanoj kisloty. Rezul'tirujushhuju smes' aminokislot razdeljali metodom hromatografii na kolonke. Selenometionin -selen-75, podgotovlennyj takim obrazom, imel biologicheskie svojstva prirodnyh aminokislot. Pogloshhenie v podzheludochnoj zheleze sostavljalo okolo 7%vvedennoj dozy, a koncentracija na gramm podzheludochnoj zhelezy byla v 7 raz bol'she chem na gramm pecheni. Pogloshhenie v krovi i drugih organah bylo eshhe nizhe, pecheni. Pogloshhenie v krovi i drugih organah bylo eshhe nizhe. V nastojashhee vremja proizvoditsja klinicheskaja ocenka metodov skennirovanija podzheludochnoj zhelezy. Opuholi podzheludochnoj zhelezy predstavljali uchastki s men'shim pogloshheniem. Ne nabljudalos' koncentracii selenometionina v sluchajah ostrogo pankreatita. Eshhe slishkom rano davat' statisticheskuju ocenku jetogo metoda obnaruzhenija opuholej podzheludochnoj zhelezy. Predpolagaemaja obshhaja doza, poluchaemaja organizmom v rezul'tate jetoj procedury, sostavljaet menee 1 rada. Doza selena sostavljaet neskol'ko mikrogramm i ne predstavljaet opasnosti v otnoshenii toksichnosti. (author)}
place = {IAEA}
year = {1964}
month = {Oct}
}