Abstract
Previous comparison in this laboratory of tissue alterations in dogs produced by exposure to 250 kVp X-rays and pulsed fission-spectrum neutrons has shown general similarity of response. However, the lungs of animals exposed to fission-spectrum neutrons are considerably more susceptible to peribronchial and perivascular pulmonary haemorrhage. An attempt has been made to define the pathogenesis of this lesion by exposing five beagles to 400 rad (MAD) fast neutrons from a bare GODIVA-type reactor and serially sacrificing the animals at periodic intervals. Gross pathologic alterations consisting of occasional small peripheral-pulmonary haemorrhages were discernible at +5 d post-irradiation. These became progressively more numerous by the ninth day. The most striking changes occurred at +13 d, consisting of extensive perivascular haemorrhage around pulmonary-arterial branches from the hilum to the pleural suriace. The earliest histologic changes observed in the lungs occurred at +5 d, consisting of focal well-demarcated areas of alveolar congestion, swelling of capillary-endothelial cells and slight alveolar extravasation of red blood cells. These changes became more generalized by + 9 d and in addition there were focal areas of peribronchial extravasation of blood involving the right and left main-stem bronchi. Animals necropsied on the thirteenth day post-irradiation revealed extensive perivascular haemorrhage involving
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Citation Formats
Jones, R. K., Engel, R. E., and Godden, W. R.
Pathogenesis of Intrapulmonary Haemorrhage in Dogs Exposed to Pulsed Fission-Spectrum Neutrons; Pathogenese de l'Hemorragie Intrapulmonaire chez des Chiens Exposes a des Neutrons de Fission Pulses; Patogenez vnutrilegochnykh krovoizliyanij u sobak pri obluchenii impul'snymi nejtronami spektra deleniya; Patogenesis de la Hemorragia Intrapulmonar en Perros Expuestos a Neutrones de Fision Pulsados.
IAEA: N. p.,
1964.
Web.
Jones, R. K., Engel, R. E., & Godden, W. R.
Pathogenesis of Intrapulmonary Haemorrhage in Dogs Exposed to Pulsed Fission-Spectrum Neutrons; Pathogenese de l'Hemorragie Intrapulmonaire chez des Chiens Exposes a des Neutrons de Fission Pulses; Patogenez vnutrilegochnykh krovoizliyanij u sobak pri obluchenii impul'snymi nejtronami spektra deleniya; Patogenesis de la Hemorragia Intrapulmonar en Perros Expuestos a Neutrones de Fision Pulsados.
IAEA.
Jones, R. K., Engel, R. E., and Godden, W. R.
1964.
"Pathogenesis of Intrapulmonary Haemorrhage in Dogs Exposed to Pulsed Fission-Spectrum Neutrons; Pathogenese de l'Hemorragie Intrapulmonaire chez des Chiens Exposes a des Neutrons de Fission Pulses; Patogenez vnutrilegochnykh krovoizliyanij u sobak pri obluchenii impul'snymi nejtronami spektra deleniya; Patogenesis de la Hemorragia Intrapulmonar en Perros Expuestos a Neutrones de Fision Pulsados."
IAEA.
@misc{etde_22141857,
title = {Pathogenesis of Intrapulmonary Haemorrhage in Dogs Exposed to Pulsed Fission-Spectrum Neutrons; Pathogenese de l'Hemorragie Intrapulmonaire chez des Chiens Exposes a des Neutrons de Fission Pulses; Patogenez vnutrilegochnykh krovoizliyanij u sobak pri obluchenii impul'snymi nejtronami spektra deleniya; Patogenesis de la Hemorragia Intrapulmonar en Perros Expuestos a Neutrones de Fision Pulsados}
author = {Jones, R. K., Engel, R. E., and Godden, W. R.}
abstractNote = {Previous comparison in this laboratory of tissue alterations in dogs produced by exposure to 250 kVp X-rays and pulsed fission-spectrum neutrons has shown general similarity of response. However, the lungs of animals exposed to fission-spectrum neutrons are considerably more susceptible to peribronchial and perivascular pulmonary haemorrhage. An attempt has been made to define the pathogenesis of this lesion by exposing five beagles to 400 rad (MAD) fast neutrons from a bare GODIVA-type reactor and serially sacrificing the animals at periodic intervals. Gross pathologic alterations consisting of occasional small peripheral-pulmonary haemorrhages were discernible at +5 d post-irradiation. These became progressively more numerous by the ninth day. The most striking changes occurred at +13 d, consisting of extensive perivascular haemorrhage around pulmonary-arterial branches from the hilum to the pleural suriace. The earliest histologic changes observed in the lungs occurred at +5 d, consisting of focal well-demarcated areas of alveolar congestion, swelling of capillary-endothelial cells and slight alveolar extravasation of red blood cells. These changes became more generalized by + 9 d and in addition there were focal areas of peribronchial extravasation of blood involving the right and left main-stem bronchi. Animals necropsied on the thirteenth day post-irradiation revealed extensive perivascular haemorrhage involving large and small pulmonaryarterial vessels. The blood appeared within the advential-connective tissue and periarterial-lymphatic spaces. Endothelial swelling was extremely marked in affected vessels, but the media appeared essentially unremarkable. Peribronchial haemorrhage was also present adjacent to affected pulmonary-arterial branches. The pathogenesis of these alterations appears to be related to primary endothelial damage produced by pulsed fission-spectrum neutrons. An additional contributing factor is probably thrombocytopenia as the number of marrow megakaryocytes was found to be inversely proportional to the severity of intrapulmonary haemorrhage. (author) [French] Des comparaisons precedemment effectuees dans le laboratoire des auteurs entre les modifications tissulaires observees chez des chiens exposes a des rayons X de 250 kV-crete et a des neutrons de fission puises ont montre que, dans les deux cas, les reactions etaient en general semblables. Cependant, les poumons des animaux exposes aux neutrons de fission ont ete beaucoup plus souvent le siege d'hemorragies peribronchiales et peri vasculaires. On a essaye de determiner la pathogenese de cette lesion en exposant cinq bigles a une dose de 400 rad (DMA) de neutronsrapides emanant d'un reacteur du type GODIVA et en sacrifiant successivement les animaux a intervalles reguliers. Cinq jours apres l 'exposition, on a pu discerner un ensemble d'alterations pathologiques constituees par de petites hemorragies pulmonaires sporadiques a la peripherie. Leur nombre a augmente progressivement vers le neuvieme jour. Les modifications les plus frappantes sont survenues au bout de 13 j sous forme de grandes hemorragies perivasculaires autour des ramuscules des arteres pulmonaires, depuis le hile jusqu' a la suriace de la plevre. Les premieres modifications histologiques observees dans les poumons sont survenues le cinquieme jour, sour forme de foyers nettement demarques de congestion alveolaire,- de gonflement des cellules capillaires endotheliales et d'extravasion alveolaire legere des globules rouges. Ces alterations se sont generalisees au bout de neuf jours et on.a pu observer en outre des foyers d'extravasion peribronchiale du sang autour des deux bronches primaires. On a constate, chez les animaux autopsies le treizieme jour apres l ' exposition, une grosse hemorragie-peri vasculaire autour des grandes et des petites arteres pulmonaires. Le sang se trouvait dans le tissu conjonctif externe et dans les vaisseaux lymphatiques periarteriels. Les vaisseaux atteints presentaient un gonflement endothelial extremement marque, mais leur tunique moyenne ne presentait pas de modifications essentielles. On notait egalement une hemorragie peribronchiale au niveau des arteres pulmonaires atteintes. La pathogenese de ces alterations semble etre liee primitivement a une deterioration de l 'endothelium provoquee par les neutrons de fission puises; un facteur complementaire est vraisemblablement la thrombocytopenie, puisqu'on a constate que le nombre de megacaryocytes de la moelle etait inversement proportionnel a'la gravite de l'hemorragie intiapulmonaire. (author) [Spanish] La comparacion de las alteraciones de los tejidos en perros expuestos a rayos X de 250 kVp y a un haz pulsado de neutrones de fision, efectuada anteriormente en el mismo laboratorio, revelo que las reacciones son similares en lineas generales. Sin embargo, los pulmones de los animales expuestos a neutrones de fision son mucho mas propensos a las hemorragias peribronquiales y perivasculares. Los autores han intentado definir la patogenesis de esta lesion exponiendo 5 sabuesos a una dosis de 400 rad (dosis maxima admisible) de un haz de neutrones rapidos procedentes de un reactor tipo GODIVA (sin reflector) y sacrificando luego los animales a intervalos regulares. Cinco dias despues de la irradiacion, se manifestaron serias alteraciones patologicas constituidas por pequenas hemorragias pulmonares esporadicas en la periferia. Hacia el noveno dia, el numero de estas hemorragias aumenta progresivamente. Los cambios mas notables ocurren a los 13 dias y consisten en extensas hemorragias perivasculares en torno a las ramas de la arteria pulmonar, desde el hilio hasta la superficie de la pleura. Las primeras modificaciones histologicas en los pulmones se observaron a los cinco dias: se trata de zonas focales bien delimitadas de congestion alveolar, acompanadas por una hinchazon de las celulas del endotelio capilar y una leve extravasacion alveolar de los hematies. Estos cambios se generalizan aun mas a los nueve dias, observandose tambien zonas focales de extravasacion peribronquial de la sangre alrededor de los dos bronquios primarios. La autopsia al decimotercer dia de la irradiacion revelo una extensa hemorragia perivascular que comprende las arterias pulmonares gruesas y finas. La sangre aparece dentro del tejido conjuntivo adventicio y de los espacios linfaticos periarteriales. La hinchazon del endotelio es muy pronunciada en los vasos afectados, pero la zona media de estos no presenta modificaciones esenciales. Tambien se manifiesta una hemorragia peribronquial contigua a las ramas afectadas de la arteria pulmonar. La patogenesis de estas alteraciones guarda al parecer una relaciuen con una lesion endotelial primaria producida por los neutrones de fision pulsados. Es probable que tambien actue como factor complementario una trombocitopenia, ya que se comprobo que el numero de megacariocitos medulares es inversamente proporcional a la gravedad de la hemorragia intrapulmonar. (author) [Russian] Predydushhie issledovanija v dannoj laboratorii po tkanevym povrezhdenijam u sobak, vyzyvaemym rentgenovskim oblucheniem s pikovym naprjazheniem 250 kv i impul'snymi nejtronami spektra delenija pokazali obshhee shodstvo reakcii. Odnako u zhivotnyh, obluchennyh nejtronami spektra delenija, chashhe v o z nikajut peribronhial'nye i perivaskuljarnye legochnye krovoizlijanija. Sdelana popytka opredelit' patogenez jetih porazhenij putem obluchenija pjati gonchih sobak dozoj v 400 rad bystryh nejtronov na reaktore tipa GODIVA bez otrazhatelja i serijnogo zabivanija zhivotnyh cherez opredelennye intervaly. Ch erez +5 dnej posle obluchenija mozhno bylo videt' znachitel'nye patologicheskie iz menenija, sostojashhie iz redkih melkih perifericheskih l egochnyh krovoizlijanij. K 9-mu dnju oni stanovilis' bolee mnogochislennymi. Naibolee porazitel'nye izmenenija, nabljudavshiesja k + 13-mu dnju, sostojali iz obshirnyh perivaskuljarnyh krovoizlijanij vokrug v et vej legochnoj arterii ot vorot legkih do poverhnosti plevry. Samye rannie gistologicheskie izmenenija v le gk i h nabljudalis' na + 5 -j den' i s o stojali iz fokusnyh, horosho ogranichennyh uchastkov giperemirovannyh a l'v eo l , otechnost' kletok jendotileja kapilljarov i vyhozhdenija jeritrocitov v nebol'shom kolichestve v polosti a l'veol. Jeti izmenenija stanovilis' generalizovannymi k + 9 -m u dnju i, krome t o go , nabljudalis' fokusnye uchastki peribronhial'nyh krovoizlijanij s vovlecheniem levogo i prav ogo glavnogo bronhov. Pri vskrytii zhivotnyh na 13-j den' posle obluchenija obnaruzheny obshirnye perivaskuljarnye krovoizlijanija s vovlecheniem krupnyh i melkih legochnyh arterial'nyh so sudov. Krov' pojavljalas' v adventicial'noj soedinitel'noj tkani i periarteri- al'nyh limfaticheskih prostranstvah. Otek jendotelija byl rezko vyrazhen v porazhennyh sosudah, no v sredinnoj obolochke zametnyh izmenenij ne bylo. Obnaruzhivalis' takzhe peribronhial'nye krovoizlijanija v sochetanii s porazheniem vetvej le gochn oj arterii. Patogenez jetih povrezhdenij, po-vidimomu, svjazan s pervichnym porazheniem jendotelija, vyzvannym impul'snymi nejtronami spektra delenija. Dopolnitel'nym faktorom, vozmozhno, javljaetsja trombocitopenija, poskol'ku okazalos', chto kolichestvo megakariocitov v kostnom m o z gu bylo obratno proporcional'no tjazhesti vnutrilegochnyh krovoizlijanij. (author)}
place = {IAEA}
year = {1964}
month = {May}
}
title = {Pathogenesis of Intrapulmonary Haemorrhage in Dogs Exposed to Pulsed Fission-Spectrum Neutrons; Pathogenese de l'Hemorragie Intrapulmonaire chez des Chiens Exposes a des Neutrons de Fission Pulses; Patogenez vnutrilegochnykh krovoizliyanij u sobak pri obluchenii impul'snymi nejtronami spektra deleniya; Patogenesis de la Hemorragia Intrapulmonar en Perros Expuestos a Neutrones de Fision Pulsados}
author = {Jones, R. K., Engel, R. E., and Godden, W. R.}
abstractNote = {Previous comparison in this laboratory of tissue alterations in dogs produced by exposure to 250 kVp X-rays and pulsed fission-spectrum neutrons has shown general similarity of response. However, the lungs of animals exposed to fission-spectrum neutrons are considerably more susceptible to peribronchial and perivascular pulmonary haemorrhage. An attempt has been made to define the pathogenesis of this lesion by exposing five beagles to 400 rad (MAD) fast neutrons from a bare GODIVA-type reactor and serially sacrificing the animals at periodic intervals. Gross pathologic alterations consisting of occasional small peripheral-pulmonary haemorrhages were discernible at +5 d post-irradiation. These became progressively more numerous by the ninth day. The most striking changes occurred at +13 d, consisting of extensive perivascular haemorrhage around pulmonary-arterial branches from the hilum to the pleural suriace. The earliest histologic changes observed in the lungs occurred at +5 d, consisting of focal well-demarcated areas of alveolar congestion, swelling of capillary-endothelial cells and slight alveolar extravasation of red blood cells. These changes became more generalized by + 9 d and in addition there were focal areas of peribronchial extravasation of blood involving the right and left main-stem bronchi. Animals necropsied on the thirteenth day post-irradiation revealed extensive perivascular haemorrhage involving large and small pulmonaryarterial vessels. The blood appeared within the advential-connective tissue and periarterial-lymphatic spaces. Endothelial swelling was extremely marked in affected vessels, but the media appeared essentially unremarkable. Peribronchial haemorrhage was also present adjacent to affected pulmonary-arterial branches. The pathogenesis of these alterations appears to be related to primary endothelial damage produced by pulsed fission-spectrum neutrons. An additional contributing factor is probably thrombocytopenia as the number of marrow megakaryocytes was found to be inversely proportional to the severity of intrapulmonary haemorrhage. (author) [French] Des comparaisons precedemment effectuees dans le laboratoire des auteurs entre les modifications tissulaires observees chez des chiens exposes a des rayons X de 250 kV-crete et a des neutrons de fission puises ont montre que, dans les deux cas, les reactions etaient en general semblables. Cependant, les poumons des animaux exposes aux neutrons de fission ont ete beaucoup plus souvent le siege d'hemorragies peribronchiales et peri vasculaires. On a essaye de determiner la pathogenese de cette lesion en exposant cinq bigles a une dose de 400 rad (DMA) de neutronsrapides emanant d'un reacteur du type GODIVA et en sacrifiant successivement les animaux a intervalles reguliers. Cinq jours apres l 'exposition, on a pu discerner un ensemble d'alterations pathologiques constituees par de petites hemorragies pulmonaires sporadiques a la peripherie. Leur nombre a augmente progressivement vers le neuvieme jour. Les modifications les plus frappantes sont survenues au bout de 13 j sous forme de grandes hemorragies perivasculaires autour des ramuscules des arteres pulmonaires, depuis le hile jusqu' a la suriace de la plevre. Les premieres modifications histologiques observees dans les poumons sont survenues le cinquieme jour, sour forme de foyers nettement demarques de congestion alveolaire,- de gonflement des cellules capillaires endotheliales et d'extravasion alveolaire legere des globules rouges. Ces alterations se sont generalisees au bout de neuf jours et on.a pu observer en outre des foyers d'extravasion peribronchiale du sang autour des deux bronches primaires. On a constate, chez les animaux autopsies le treizieme jour apres l ' exposition, une grosse hemorragie-peri vasculaire autour des grandes et des petites arteres pulmonaires. Le sang se trouvait dans le tissu conjonctif externe et dans les vaisseaux lymphatiques periarteriels. Les vaisseaux atteints presentaient un gonflement endothelial extremement marque, mais leur tunique moyenne ne presentait pas de modifications essentielles. On notait egalement une hemorragie peribronchiale au niveau des arteres pulmonaires atteintes. La pathogenese de ces alterations semble etre liee primitivement a une deterioration de l 'endothelium provoquee par les neutrons de fission puises; un facteur complementaire est vraisemblablement la thrombocytopenie, puisqu'on a constate que le nombre de megacaryocytes de la moelle etait inversement proportionnel a'la gravite de l'hemorragie intiapulmonaire. (author) [Spanish] La comparacion de las alteraciones de los tejidos en perros expuestos a rayos X de 250 kVp y a un haz pulsado de neutrones de fision, efectuada anteriormente en el mismo laboratorio, revelo que las reacciones son similares en lineas generales. Sin embargo, los pulmones de los animales expuestos a neutrones de fision son mucho mas propensos a las hemorragias peribronquiales y perivasculares. Los autores han intentado definir la patogenesis de esta lesion exponiendo 5 sabuesos a una dosis de 400 rad (dosis maxima admisible) de un haz de neutrones rapidos procedentes de un reactor tipo GODIVA (sin reflector) y sacrificando luego los animales a intervalos regulares. Cinco dias despues de la irradiacion, se manifestaron serias alteraciones patologicas constituidas por pequenas hemorragias pulmonares esporadicas en la periferia. Hacia el noveno dia, el numero de estas hemorragias aumenta progresivamente. Los cambios mas notables ocurren a los 13 dias y consisten en extensas hemorragias perivasculares en torno a las ramas de la arteria pulmonar, desde el hilio hasta la superficie de la pleura. Las primeras modificaciones histologicas en los pulmones se observaron a los cinco dias: se trata de zonas focales bien delimitadas de congestion alveolar, acompanadas por una hinchazon de las celulas del endotelio capilar y una leve extravasacion alveolar de los hematies. Estos cambios se generalizan aun mas a los nueve dias, observandose tambien zonas focales de extravasacion peribronquial de la sangre alrededor de los dos bronquios primarios. La autopsia al decimotercer dia de la irradiacion revelo una extensa hemorragia perivascular que comprende las arterias pulmonares gruesas y finas. La sangre aparece dentro del tejido conjuntivo adventicio y de los espacios linfaticos periarteriales. La hinchazon del endotelio es muy pronunciada en los vasos afectados, pero la zona media de estos no presenta modificaciones esenciales. Tambien se manifiesta una hemorragia peribronquial contigua a las ramas afectadas de la arteria pulmonar. La patogenesis de estas alteraciones guarda al parecer una relaciuen con una lesion endotelial primaria producida por los neutrones de fision pulsados. Es probable que tambien actue como factor complementario una trombocitopenia, ya que se comprobo que el numero de megacariocitos medulares es inversamente proporcional a la gravedad de la hemorragia intrapulmonar. (author) [Russian] Predydushhie issledovanija v dannoj laboratorii po tkanevym povrezhdenijam u sobak, vyzyvaemym rentgenovskim oblucheniem s pikovym naprjazheniem 250 kv i impul'snymi nejtronami spektra delenija pokazali obshhee shodstvo reakcii. Odnako u zhivotnyh, obluchennyh nejtronami spektra delenija, chashhe v o z nikajut peribronhial'nye i perivaskuljarnye legochnye krovoizlijanija. Sdelana popytka opredelit' patogenez jetih porazhenij putem obluchenija pjati gonchih sobak dozoj v 400 rad bystryh nejtronov na reaktore tipa GODIVA bez otrazhatelja i serijnogo zabivanija zhivotnyh cherez opredelennye intervaly. Ch erez +5 dnej posle obluchenija mozhno bylo videt' znachitel'nye patologicheskie iz menenija, sostojashhie iz redkih melkih perifericheskih l egochnyh krovoizlijanij. K 9-mu dnju oni stanovilis' bolee mnogochislennymi. Naibolee porazitel'nye izmenenija, nabljudavshiesja k + 13-mu dnju, sostojali iz obshirnyh perivaskuljarnyh krovoizlijanij vokrug v et vej legochnoj arterii ot vorot legkih do poverhnosti plevry. Samye rannie gistologicheskie izmenenija v le gk i h nabljudalis' na + 5 -j den' i s o stojali iz fokusnyh, horosho ogranichennyh uchastkov giperemirovannyh a l'v eo l , otechnost' kletok jendotileja kapilljarov i vyhozhdenija jeritrocitov v nebol'shom kolichestve v polosti a l'veol. Jeti izmenenija stanovilis' generalizovannymi k + 9 -m u dnju i, krome t o go , nabljudalis' fokusnye uchastki peribronhial'nyh krovoizlijanij s vovlecheniem levogo i prav ogo glavnogo bronhov. Pri vskrytii zhivotnyh na 13-j den' posle obluchenija obnaruzheny obshirnye perivaskuljarnye krovoizlijanija s vovlecheniem krupnyh i melkih legochnyh arterial'nyh so sudov. Krov' pojavljalas' v adventicial'noj soedinitel'noj tkani i periarteri- al'nyh limfaticheskih prostranstvah. Otek jendotelija byl rezko vyrazhen v porazhennyh sosudah, no v sredinnoj obolochke zametnyh izmenenij ne bylo. Obnaruzhivalis' takzhe peribronhial'nye krovoizlijanija v sochetanii s porazheniem vetvej le gochn oj arterii. Patogenez jetih povrezhdenij, po-vidimomu, svjazan s pervichnym porazheniem jendotelija, vyzvannym impul'snymi nejtronami spektra delenija. Dopolnitel'nym faktorom, vozmozhno, javljaetsja trombocitopenija, poskol'ku okazalos', chto kolichestvo megakariocitov v kostnom m o z gu bylo obratno proporcional'no tjazhesti vnutrilegochnyh krovoizlijanij. (author)}
place = {IAEA}
year = {1964}
month = {May}
}