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Effects of Monoenergetic Neutron Radiation on Human Cells in Tissue Culture; Effets des Neutrons Monoenergetiques sur des Cellules de Tissus Humains en Culture; Dejstvie oblucheniya monoehnergeticheskimi nejtronami na kletki kul'tury tkanej cheloveka; Efectos de la Radiacion Neutronica Monoenergetica sobre las Celulas Humanas en Cultivos de Tejidos

Conference:

Abstract

Dose effect relations concerning inhibition of clone formation by kidney cells of human origin in culture after irradiation with monoenergetic neutrons of about 3-MeV and about 15-MeV energy, are reported. For 3-MeV neutrons the dose rate was equal to 0.2 rad/min, while for 15-MeV neutrons dose rates of 2.7 rad/min and 12 rad/min were obtained. The cells were irradiated in culture dishes with a thin Melinex bottom and in small flexible nylon and silicon rubber tubes. Neutron fluxes were determined by measuring the activation of sulphur pellets mounted respectively between the dishes and the tubes. Calculation of the dose on the basis of the neutron flux presented a number of problems which are discussed. Because no radiation equilibrium exists around the cells, the resulting inaccuracies in the dose determinations are investigated. Dose-survival curves for both types of neutrons are presented. For 3-MeV neutrons an RBE was found ranging from 6.5 at low doses to about 3.1 at high doses, while for 15-MeV neutrons the RBE varied from 1.9 to 1.6. By comparison of these RBE-values with the RBE-LET relation obtained in earlier experiments with deuterons and {alpha}-particles, it is possible to derive an effective LET for the neutrons used. In  More>>
Authors:
Broerse, J. J.; Barendsen, G. W. [1] 
  1. Radiobiological Institute of the Organization for Health Research TNO, Rijswijk (Z.H.) (Netherlands)
Publication Date:
Mar 15, 1964
Product Type:
Conference
Report Number:
IAEA-SM-44/6
Resource Relation:
Conference: Symposium on Biological Effects of Neutron Irradiations, Upton, NY (United States), 7-11 Dec 1963; Other Information: 19 refs., 4 figs., 6 tabs.; Related Information: In: Biological Effects of Neutron and Proton Irradiations. Vol. I. Proceedings of the Symposium on Biological Effects of Neutron Irradiations| 448 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL CELLS; BIOLOGICAL RADIATION EFFECTS; DEATH; DEUTERONS; IRRADIATION; KIDNEYS; LET; MEV RANGE 01-10; NEUTRON FLUX; NEUTRONS; NYLON; PHANTOMS; RADIATION DOSES; RBE; RUBBERS; SILICON; SULFUR; SURVIVAL CURVES; TISSUE CULTURES
OSTI ID:
22141843
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M3445096444
Submitting Site:
INIS
Size:
page(s) 309-322
Announcement Date:
Oct 24, 2013

Conference:

Citation Formats

Broerse, J. J., and Barendsen, G. W. Effects of Monoenergetic Neutron Radiation on Human Cells in Tissue Culture; Effets des Neutrons Monoenergetiques sur des Cellules de Tissus Humains en Culture; Dejstvie oblucheniya monoehnergeticheskimi nejtronami na kletki kul'tury tkanej cheloveka; Efectos de la Radiacion Neutronica Monoenergetica sobre las Celulas Humanas en Cultivos de Tejidos. IAEA: N. p., 1964. Web.
Broerse, J. J., & Barendsen, G. W. Effects of Monoenergetic Neutron Radiation on Human Cells in Tissue Culture; Effets des Neutrons Monoenergetiques sur des Cellules de Tissus Humains en Culture; Dejstvie oblucheniya monoehnergeticheskimi nejtronami na kletki kul'tury tkanej cheloveka; Efectos de la Radiacion Neutronica Monoenergetica sobre las Celulas Humanas en Cultivos de Tejidos. IAEA.
Broerse, J. J., and Barendsen, G. W. 1964. "Effects of Monoenergetic Neutron Radiation on Human Cells in Tissue Culture; Effets des Neutrons Monoenergetiques sur des Cellules de Tissus Humains en Culture; Dejstvie oblucheniya monoehnergeticheskimi nejtronami na kletki kul'tury tkanej cheloveka; Efectos de la Radiacion Neutronica Monoenergetica sobre las Celulas Humanas en Cultivos de Tejidos." IAEA.
@misc{etde_22141843,
title = {Effects of Monoenergetic Neutron Radiation on Human Cells in Tissue Culture; Effets des Neutrons Monoenergetiques sur des Cellules de Tissus Humains en Culture; Dejstvie oblucheniya monoehnergeticheskimi nejtronami na kletki kul'tury tkanej cheloveka; Efectos de la Radiacion Neutronica Monoenergetica sobre las Celulas Humanas en Cultivos de Tejidos}
author = {Broerse, J. J., and Barendsen, G. W.}
abstractNote = {Dose effect relations concerning inhibition of clone formation by kidney cells of human origin in culture after irradiation with monoenergetic neutrons of about 3-MeV and about 15-MeV energy, are reported. For 3-MeV neutrons the dose rate was equal to 0.2 rad/min, while for 15-MeV neutrons dose rates of 2.7 rad/min and 12 rad/min were obtained. The cells were irradiated in culture dishes with a thin Melinex bottom and in small flexible nylon and silicon rubber tubes. Neutron fluxes were determined by measuring the activation of sulphur pellets mounted respectively between the dishes and the tubes. Calculation of the dose on the basis of the neutron flux presented a number of problems which are discussed. Because no radiation equilibrium exists around the cells, the resulting inaccuracies in the dose determinations are investigated. Dose-survival curves for both types of neutrons are presented. For 3-MeV neutrons an RBE was found ranging from 6.5 at low doses to about 3.1 at high doses, while for 15-MeV neutrons the RBE varied from 1.9 to 1.6. By comparison of these RBE-values with the RBE-LET relation obtained in earlier experiments with deuterons and {alpha}-particles, it is possible to derive an effective LET for the neutrons used. In order to elucidate certain problems related to the death of experimental animals after X- or neutron- irradiation, a technique has been developed whereby the cells, contained in small tubes, are used to measure the effective dose (i.e . dose corrected for the RBE) at sites of interest in phantoms or animals. (author) [French] Les auteurs indiquent les relations dose-effet pour l 'inhibition de la formation de lignees par les cellules renales d' origine humaine apres irradiation par des neutrons monoenergetiques de 3 MeV et de 15 MeV. Pour les neutrons de 3 MeV, le debit de dose etait egal a 0,2 rad/min, alors que pour les neutrons de 15 MeV on a obtenu des debits de dose de 2,7 rad/min et de 12 rad/min. L'irradiation des cellules avait lieu dans des bottes de culture a fond mince en Melinex et dans de petits tubes flexibles en nylon et en caoutchouc au silicium. Les auteurs ont determine les flux de neutrons en mesurant l'activation de pastilles de soufre placees, selon les cas, entre les boftes ou entre les tubes. Ils examinent les divers problemes que pose le calcul de la dose d' apres le flux de neutrons. Comme il n'y a pas equilibre du rayonnement autour des cellules, ils analysent les inexactitudes qui en resultent dans les determinations de la dose. Le memoire donne les courbes dose-survie pour les neutrons des deux types. Pour les neutrons de 3 MeV, on a trouve une EBR allant de 6, 5 dans le cas de faibles doses a 3,1 dans le cas de doses elevees; pour les neutrons de 15 MeV, l 'EBR variait de 1,9 a 1,6. En comparant ces valeurs de l'EBR avec le rapport EBR-TLE obtenu au cours d'experiences precedentes avec des deuterons et des particules alpha, on peut calculer le TLE effectif pour les neutrons utilises. Pour resoudre certains problemes relatifs a la mort des animaux d'experience apres exposition aux rayons X ou aux neutrons, les auteurs ont mis au point une technique permettant d'utiliser les cellules contenues dans de petits tubes pour mesurer la dose effective (c'est-a-dire la dose corrigee en fonction de l'EBR) en des points interessants dans des fantomes ou des animaux. (author) [Spanish] Los autores presentan relaciones entre dosis y efecto comprobadas en el estudio de la inhibicion de la formacion de clonos en cultivos de tejido renal humano despues de la irradiacion con neutrones monoenergeticos de unos 3 MeV y unos 15 MeV, respectivamente. Para los neutrones de 3 MeV, la intensidad de dosis fue de 0,2 rad/min, mientras que para los neutrones de 15 MeV, se alcanzaron intensidades de dosis de 2,7 rad/min y 12 rad/min. Las celulas se irradiaron en capsulas de cultivo con fondo delgado de Melinex y en tubos finos flexibles de nylon y de caucho de silicona. Los flujos neutronicos se determinaron midiendo la activacion de unas pastillas de azufre dispuestas entre las capsulas y los tubos. El calculo de las dosis a partir del flujo neutronico plantea una serie de problemas que los autores analizan. Como no se establece un equilibrio de radiacion en torno a las celulas, las respectivas determinaciones de dosis adolecen de falta de precision que se investigan en el trabajo. Los autores presentan curvas de la supervivencia en funcion de la dosis, para las dos energias neutronicas mencionadas. Con los neutrones de 3 MeV, comprobaron que la EBR varia desde 6,5 (dosis reducidas) hasta alrededor de 3,1 (dosis elevadas); con los neutrones de 15 MeV, la EBR varia entre 1,9 y 1,6. Combinando estos valores de la EBR con la relacion entre la TLE y la EBR establecida en experimentos previos con deuterones y particulas {alpha}, se pueden deducir valores de la TLE efectiva correspondientes a los neutrones utilizados. A fin de dilucidar algunos problemas relacionados con la muerte de animales de laboratorio sometidos a la accion de los rayos X o de los neutrones, los autores perfeccionaron un procedimiento basado en el empleo de celulas, contenidas en pequeflos tubos a fin de medir la dosis efectiva (es decir, la dosis corregida para tener en cuenta la EBR) en zonas determinadas de maniquies o de animales, que ofrecen interes para estos estudios. (author) [Russian] Soobshhaetsja o sootnoshenii doza-jeffekt na osnovanii izluchenija tormozhenija obrazovanija klona pochechnymi kletkami cheloveka posle obluchenija monojenergeticheskimi nejtronami s jenergiej primerno 3 Mjev i 15 Mjev. Dlja nejtronov s jenergiej 3 Mjev moshhnost' dozy ravnjalas' 0,2 rad/min, v to vremja kak dlja nejtronov s jenergiej v 15 Mjev byli polucheny moshhnosti dozy v 2,7 rad/min i 12 rad/min. Obluchenie kletok proizvodilos' v chashkah dlja kul'tury tkanej s tonkim dnom iz stekla Melimeks i v nebol'shih gibkih nejlonovyh rezinovyh trubkah. Potoki nejtronov opredeljalis' putem izmerenija aktivacii sernyh sharikov, pomeshhennyh sootvetstvenno mezhdu chashkami i trubkami. R a sche t dozy nejtronnogo potoka predstavljal rjad problem, kotorye obsuzhdajutsja. Poskol'ku ne sushhestvuet ravnomernogo obluchenija kletok, izuchajutsja rezul'tirujushhie netochnosti pri opredelenijah dozy. Privodjatsja krivye zavisimosti vyzhivanija ot dozy dlja oboih tipov nejtronov. Dlja nejtronov s jenergiej 3 Mjev otnositel'naja biologicheskaja jeffektivnost' sostavljala ot 6,5 pri malyh dozah do primerno 3,1 pri vysokih d o zah , v to vremja kak dlja nejtronov s jenergiej 15 Mjev velichiny OBJe ko lebalas' ot 1,9 do 1,6. Putem sravnenija jetih velichin OBJe s s o otnosheniem O B Je-LP Je , poluchennym v predydushhih jeksperimentah s dejtronami i al'f a - chasticami, mozhno opredelit' jeffektivnoe LPJe dlja nejtronov, ispol'zovannyh v jeksperimente. Dlja reshenija nekotoryh problem, svjazannyh so smert'ju jeksperimental'nyh zhivotnyh posle rentgenovskogo ili nejtronnogo obluchenija, byl razrabotan metod, s pomoshh'ju kotorogo kletki, soderzhashhiesja v nebol'shih trubkah, ispol'zujutsja dlja izmerenija jeffektivnoj dozy (t.e. dozy, dlja kotoroj vneseny popravki na OBJe) v uchastkah fantomov ili t el zhivotnyh, predstavljajushhih interes. (author)}
place = {IAEA}
year = {1964}
month = {Mar}
}