Abstract
The aim of this study was to optimize the parameters of moisture, temperature and ratio of nutrients to estimate the possibility of applying the technique of bioremediation in a soil contaminated with hydrocarbons. For this, an initial characterization of contaminated soil was made according to their physical and chemical characteristics and the number of heterotrophic and hydrocarbon degraders bacteria. Also the contaminant concentration by gravimetric method and by gas chromatography was studied. To optimize moisture and temperature, microcosms with moisture of 3%, 10%, 15% and 20% and temperatures of 5, 15, 28 and 37 degrees Celsius were used. The monitoring of the mineralization of hydrocarbons was performed by measuring the CO{sub 2} produced. To optimize the ratio of nutrients, different microcosms were designed and were monitored by oxygen consumption and by determination of hydrocarbons by gas chromatography. The C:N:P relationships studied were 100:20:2, 100:10:1, 100:5:0,5 and 100:1:0,1. The results indicate that the mineralization of hydrocarbons was optimal for moisture of 10% to 20% and temperatures of 25 degrees Celsius to 37 degrees Celsius with CO{sub 2} production values of 3000-4500 mg CO{sub 2} kg-1. The optimal C:N:P ratio was 100:1:0,1 in which the highest oxygen consumption was and the elimination
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Acuna, A. J.;
Tonin, N. L.;
Diaz, V.;
Pucci, G. N.;
Pucci, O. H.;
[1]
ar, ntonin@unpata. edu.;
com, victordj_2003@hotmail.;
ar, granapu@unpata. edu.;
ar, ceima@unpata. edu.
- Centro de Estudios e Investigacion en Microbiologia Aplicada (CEIMA), Universidad Nacional de la Patagonia San Juan Bosco (Argentina)
Citation Formats
Acuna, A. J., Tonin, N. L., Diaz, V., Pucci, G. N., Pucci, O. H., ar, ntonin@unpata. edu., com, victordj_2003@hotmail., ar, granapu@unpata. edu., and ar, ceima@unpata. edu.
Optimization of a hydrocarbon bioremediation system at laboratory scale; Optimizacion de un sistema de biorremediacion de hidrocarburos a escala de laboratorio.
Mexico: N. p.,
2012.
Web.
Acuna, A. J., Tonin, N. L., Diaz, V., Pucci, G. N., Pucci, O. H., ar, ntonin@unpata. edu., com, victordj_2003@hotmail., ar, granapu@unpata. edu., & ar, ceima@unpata. edu.
Optimization of a hydrocarbon bioremediation system at laboratory scale; Optimizacion de un sistema de biorremediacion de hidrocarburos a escala de laboratorio.
Mexico.
Acuna, A. J., Tonin, N. L., Diaz, V., Pucci, G. N., Pucci, O. H., ar, ntonin@unpata. edu., com, victordj_2003@hotmail., ar, granapu@unpata. edu., and ar, ceima@unpata. edu.
2012.
"Optimization of a hydrocarbon bioremediation system at laboratory scale; Optimizacion de un sistema de biorremediacion de hidrocarburos a escala de laboratorio."
Mexico.
@misc{etde_22134376,
title = {Optimization of a hydrocarbon bioremediation system at laboratory scale; Optimizacion de un sistema de biorremediacion de hidrocarburos a escala de laboratorio}
author = {Acuna, A. J., Tonin, N. L., Diaz, V., Pucci, G. N., Pucci, O. H., ar, ntonin@unpata. edu., com, victordj_2003@hotmail., ar, granapu@unpata. edu., and ar, ceima@unpata. edu.}
abstractNote = {The aim of this study was to optimize the parameters of moisture, temperature and ratio of nutrients to estimate the possibility of applying the technique of bioremediation in a soil contaminated with hydrocarbons. For this, an initial characterization of contaminated soil was made according to their physical and chemical characteristics and the number of heterotrophic and hydrocarbon degraders bacteria. Also the contaminant concentration by gravimetric method and by gas chromatography was studied. To optimize moisture and temperature, microcosms with moisture of 3%, 10%, 15% and 20% and temperatures of 5, 15, 28 and 37 degrees Celsius were used. The monitoring of the mineralization of hydrocarbons was performed by measuring the CO{sub 2} produced. To optimize the ratio of nutrients, different microcosms were designed and were monitored by oxygen consumption and by determination of hydrocarbons by gas chromatography. The C:N:P relationships studied were 100:20:2, 100:10:1, 100:5:0,5 and 100:1:0,1. The results indicate that the mineralization of hydrocarbons was optimal for moisture of 10% to 20% and temperatures of 25 degrees Celsius to 37 degrees Celsius with CO{sub 2} production values of 3000-4500 mg CO{sub 2} kg-1. The optimal C:N:P ratio was 100:1:0,1 in which the highest oxygen consumption was and the elimination of 83% of total hydrocarbons determined by gas chromatography with 78% and 89% of n-alkanes and polyaromatic hydrocarbons elimination, respectively. [Spanish] El objeto de este trabajo fue optimizar los parametros de humedad, temperatura y relacion de nutrientes para estimar la posibilidad de aplicar la tecnica de biorremediacion en un suelo contaminado con hidrocarburos. Para esto se realizo una caracterizacion inicial del suelo contaminado en funcion de sus caracteristicas fisicas y quimicas, su cantidad de microorganismos heterotrofos y degradadores de hidrocarburos. Tambien se estudio la concentracion del contaminante por metodo gravimetrico y por cromatografia gaseosa. Para optimizar humedad y temperatura se trabajo en microcosmos con humedades de 3%, 10%, 15% y 20% y temperaturas de 5, 15, 28 y 37 grados centigrados, realizando un seguimiento de la mineralizacion de los hidrocarburos midiendo el CO{sub 2} producido. Para optimizar la relacion de nutrientes se disenaron microcosmos diferentes que fueron monitoreados por consumo de oxigeno y por determinacion de hidrocarburos por cromatografia gaseosa. Las relaciones C:N:P estudiadas fueron 100:20:2, 100:10:1, 100:5:0,5 y 100:1:0,1. Los resultados obtenidos indican que la mineralizacion de hidrocarburos fue optima para humedades de 10% a 20% y a temperaturas de 25 a 37 grados centigrados con valores de produccion de CO{sub 2} de 3000 a 4500 mg CO{sub 2} kg-1. La relacion optima C:N:P fue de 100:1:0,1 en la que se observo el mayor consumo de oxigeno y la remocion de 83% de los hidrocarburos totales determinados por cromatografia gaseosa, con 78% y 89% de eliminacion de los hidrocarburos n-alcanos y poliaromaticos, respectivamente.}
journal = []
issue = {1}
volume = {13}
journal type = {AC}
place = {Mexico}
year = {2012}
month = {Jan}
}
title = {Optimization of a hydrocarbon bioremediation system at laboratory scale; Optimizacion de un sistema de biorremediacion de hidrocarburos a escala de laboratorio}
author = {Acuna, A. J., Tonin, N. L., Diaz, V., Pucci, G. N., Pucci, O. H., ar, ntonin@unpata. edu., com, victordj_2003@hotmail., ar, granapu@unpata. edu., and ar, ceima@unpata. edu.}
abstractNote = {The aim of this study was to optimize the parameters of moisture, temperature and ratio of nutrients to estimate the possibility of applying the technique of bioremediation in a soil contaminated with hydrocarbons. For this, an initial characterization of contaminated soil was made according to their physical and chemical characteristics and the number of heterotrophic and hydrocarbon degraders bacteria. Also the contaminant concentration by gravimetric method and by gas chromatography was studied. To optimize moisture and temperature, microcosms with moisture of 3%, 10%, 15% and 20% and temperatures of 5, 15, 28 and 37 degrees Celsius were used. The monitoring of the mineralization of hydrocarbons was performed by measuring the CO{sub 2} produced. To optimize the ratio of nutrients, different microcosms were designed and were monitored by oxygen consumption and by determination of hydrocarbons by gas chromatography. The C:N:P relationships studied were 100:20:2, 100:10:1, 100:5:0,5 and 100:1:0,1. The results indicate that the mineralization of hydrocarbons was optimal for moisture of 10% to 20% and temperatures of 25 degrees Celsius to 37 degrees Celsius with CO{sub 2} production values of 3000-4500 mg CO{sub 2} kg-1. The optimal C:N:P ratio was 100:1:0,1 in which the highest oxygen consumption was and the elimination of 83% of total hydrocarbons determined by gas chromatography with 78% and 89% of n-alkanes and polyaromatic hydrocarbons elimination, respectively. [Spanish] El objeto de este trabajo fue optimizar los parametros de humedad, temperatura y relacion de nutrientes para estimar la posibilidad de aplicar la tecnica de biorremediacion en un suelo contaminado con hidrocarburos. Para esto se realizo una caracterizacion inicial del suelo contaminado en funcion de sus caracteristicas fisicas y quimicas, su cantidad de microorganismos heterotrofos y degradadores de hidrocarburos. Tambien se estudio la concentracion del contaminante por metodo gravimetrico y por cromatografia gaseosa. Para optimizar humedad y temperatura se trabajo en microcosmos con humedades de 3%, 10%, 15% y 20% y temperaturas de 5, 15, 28 y 37 grados centigrados, realizando un seguimiento de la mineralizacion de los hidrocarburos midiendo el CO{sub 2} producido. Para optimizar la relacion de nutrientes se disenaron microcosmos diferentes que fueron monitoreados por consumo de oxigeno y por determinacion de hidrocarburos por cromatografia gaseosa. Las relaciones C:N:P estudiadas fueron 100:20:2, 100:10:1, 100:5:0,5 y 100:1:0,1. Los resultados obtenidos indican que la mineralizacion de hidrocarburos fue optima para humedades de 10% a 20% y a temperaturas de 25 a 37 grados centigrados con valores de produccion de CO{sub 2} de 3000 a 4500 mg CO{sub 2} kg-1. La relacion optima C:N:P fue de 100:1:0,1 en la que se observo el mayor consumo de oxigeno y la remocion de 83% de los hidrocarburos totales determinados por cromatografia gaseosa, con 78% y 89% de eliminacion de los hidrocarburos n-alcanos y poliaromaticos, respectivamente.}
journal = []
issue = {1}
volume = {13}
journal type = {AC}
place = {Mexico}
year = {2012}
month = {Jan}
}