Abstract
Populations of amphibians are in decline in certain locations around the world, and the possible contribution of environmental contaminants, including estrogenic compounds, to these declines is of potential concern. In the current study, responses of the wood frog (Lithobates sylvaticus) to exposure to 17{alpha}-ethynylestradiol (EE2), the synthetic estrogen used in oral contraceptives, during the larval period were characterized. Exposure of L. sylvaticus to 1.08, 9.55, or 80.9 {mu}g EE2/L had no effects on survival, growth, or metamorphic endpoints monitored in the current study. However, there were significant effects of exposure to EE2 on phenotypic sex ratios. In general, lesser proportions of L. sylvaticus developed as phenotypic males and greater proportions developed as phenotypic females or with mixed sex phenotypes at all concentrations of EE2 tested. Utilizing the data collected in the current study, the EC{sub 50} for complete feminization of L. sylvaticus was determined to be 7.7 {mu}g EE2/L, and the EC{sub 50} for partial feminization was determined to be 2.3 {mu}g EE2/L. In addition, after chronic exposure, abundances of transcripts of vitellogenin A2, high density lipoprotein binding protein, and 7-dehydrocholesterol reductase were 1.8-280-fold greater in livers from L. sylvaticus exposed to EE2 compared to controls. Overall, there were significant
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Tompsett, Amber R., E-mail: amber.tompsett@usask.ca;
[1]
Wiseman, Steve;
Higley, Eric;
[1]
Giesy, John P.;
[1]
Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada);
Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)];
Hecker, Markus;
[1]
School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)]
- Toxicology Centre, University of Saskatchewan, Saskatoon, SK (Canada)
Citation Formats
Tompsett, Amber R., E-mail: amber.tompsett@usask.ca, Wiseman, Steve, Higley, Eric, Giesy, John P., Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada), Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)], Hecker, Markus, and School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)].
Effects of exposure to 17{alpha}-ethynylestradiol during larval development on growth, sexual differentiation, and abundances of transcripts in the liver of the wood frog (Lithobates sylvaticus).
Netherlands: N. p.,
2013.
Web.
doi:10.1016/J.AQUATOX.2012.10.003.
Tompsett, Amber R., E-mail: amber.tompsett@usask.ca, Wiseman, Steve, Higley, Eric, Giesy, John P., Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada), Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)], Hecker, Markus, & School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)].
Effects of exposure to 17{alpha}-ethynylestradiol during larval development on growth, sexual differentiation, and abundances of transcripts in the liver of the wood frog (Lithobates sylvaticus).
Netherlands.
https://doi.org/10.1016/J.AQUATOX.2012.10.003
Tompsett, Amber R., E-mail: amber.tompsett@usask.ca, Wiseman, Steve, Higley, Eric, Giesy, John P., Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada), Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)], Hecker, Markus, and School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)].
2013.
"Effects of exposure to 17{alpha}-ethynylestradiol during larval development on growth, sexual differentiation, and abundances of transcripts in the liver of the wood frog (Lithobates sylvaticus)."
Netherlands.
https://doi.org/10.1016/J.AQUATOX.2012.10.003.
@misc{etde_22133550,
title = {Effects of exposure to 17{alpha}-ethynylestradiol during larval development on growth, sexual differentiation, and abundances of transcripts in the liver of the wood frog (Lithobates sylvaticus)}
author = {Tompsett, Amber R., E-mail: amber.tompsett@usask.ca, Wiseman, Steve, Higley, Eric, Giesy, John P., Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada), Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)], Hecker, Markus, and School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)]}
abstractNote = {Populations of amphibians are in decline in certain locations around the world, and the possible contribution of environmental contaminants, including estrogenic compounds, to these declines is of potential concern. In the current study, responses of the wood frog (Lithobates sylvaticus) to exposure to 17{alpha}-ethynylestradiol (EE2), the synthetic estrogen used in oral contraceptives, during the larval period were characterized. Exposure of L. sylvaticus to 1.08, 9.55, or 80.9 {mu}g EE2/L had no effects on survival, growth, or metamorphic endpoints monitored in the current study. However, there were significant effects of exposure to EE2 on phenotypic sex ratios. In general, lesser proportions of L. sylvaticus developed as phenotypic males and greater proportions developed as phenotypic females or with mixed sex phenotypes at all concentrations of EE2 tested. Utilizing the data collected in the current study, the EC{sub 50} for complete feminization of L. sylvaticus was determined to be 7.7 {mu}g EE2/L, and the EC{sub 50} for partial feminization was determined to be 2.3 {mu}g EE2/L. In addition, after chronic exposure, abundances of transcripts of vitellogenin A2, high density lipoprotein binding protein, and 7-dehydrocholesterol reductase were 1.8-280-fold greater in livers from L. sylvaticus exposed to EE2 compared to controls. Overall, there were significant effects of exposure to all concentrations of EE2 tested, the least of which was within about 2-fold of estrogen equivalent concentrations previously measured in the environment.}
doi = {10.1016/J.AQUATOX.2012.10.003}
journal = []
volume = {126}
journal type = {AC}
place = {Netherlands}
year = {2013}
month = {Jan}
}
title = {Effects of exposure to 17{alpha}-ethynylestradiol during larval development on growth, sexual differentiation, and abundances of transcripts in the liver of the wood frog (Lithobates sylvaticus)}
author = {Tompsett, Amber R., E-mail: amber.tompsett@usask.ca, Wiseman, Steve, Higley, Eric, Giesy, John P., Dept. of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, SK (Canada), Dept. of Biology and Chemistry and State Key Laboratory for Marine Pollution, City University of Hong Kong, Hong Kong, Special Administrative Region (Hong Kong)], Hecker, Markus, and School of the Environment and Sustainability, University of Saskatchewan, Saskatoon, SK (Canada)]}
abstractNote = {Populations of amphibians are in decline in certain locations around the world, and the possible contribution of environmental contaminants, including estrogenic compounds, to these declines is of potential concern. In the current study, responses of the wood frog (Lithobates sylvaticus) to exposure to 17{alpha}-ethynylestradiol (EE2), the synthetic estrogen used in oral contraceptives, during the larval period were characterized. Exposure of L. sylvaticus to 1.08, 9.55, or 80.9 {mu}g EE2/L had no effects on survival, growth, or metamorphic endpoints monitored in the current study. However, there were significant effects of exposure to EE2 on phenotypic sex ratios. In general, lesser proportions of L. sylvaticus developed as phenotypic males and greater proportions developed as phenotypic females or with mixed sex phenotypes at all concentrations of EE2 tested. Utilizing the data collected in the current study, the EC{sub 50} for complete feminization of L. sylvaticus was determined to be 7.7 {mu}g EE2/L, and the EC{sub 50} for partial feminization was determined to be 2.3 {mu}g EE2/L. In addition, after chronic exposure, abundances of transcripts of vitellogenin A2, high density lipoprotein binding protein, and 7-dehydrocholesterol reductase were 1.8-280-fold greater in livers from L. sylvaticus exposed to EE2 compared to controls. Overall, there were significant effects of exposure to all concentrations of EE2 tested, the least of which was within about 2-fold of estrogen equivalent concentrations previously measured in the environment.}
doi = {10.1016/J.AQUATOX.2012.10.003}
journal = []
volume = {126}
journal type = {AC}
place = {Netherlands}
year = {2013}
month = {Jan}
}