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Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna)

Abstract

The induction of vitellogenin in oviparous vertebrates has become the gold standard biomarker of exposure to estrogenic chemicals in the environment. This biomarker of estrogen exposure also has been used in arthropods, however, little is known of the factors that regulate the expression of vitellogenin in these organisms. We investigated changes in accumulation of mRNA products of the vitellogenin gene Vtg2 in daphnids (Daphnia magna) exposed to a diverse array of chemicals. We further evaluated the involvement of hormonal factors in the regulation of vitellogenin expression that may be targets of xenobiotic chemicals. Expression of the Vtg2 gene was highly responsive to exposure to various chemicals with an expression range spanning approximately four orders of magnitude. Chemicals causing the greatest induction were piperonyl butoxide, chlordane, 4-nonylphenol, cadmium, and chloroform. Among these, only 4-nonylphenol is recognized to be estrogenic. Exposure to several chemicals also suppressed Vtg2 mRNA levels, as much as 100-fold. Suppressive chemicals included cyproterone acetate, acetone, triclosan, and atrazine. Exposure to the estrogens diethylstilbestrol and bisphenol A had little effect on vitellogenin mRNA levels further substantiating that these genes are not induced by estrogen exposure. Exposure to the potent ecdysteroids 20-hydroxyecdysone and ponasterone A revealed that Vtg2 was subject  More>>
Authors:
Hannas, Bethany R.; Wang, Ying H.; Thomson, Susanne; Kwon, Gwijun; Hong, Li; [1]  LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu [1] 
  1. Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27695-7633 (United States)
Publication Date:
Jan 25, 2011
Product Type:
Journal Article
Resource Relation:
Journal Name: Aquatic Toxicology; Journal Volume: 101; Journal Issue: 2; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ACETONE; BIOLOGICAL ACCUMULATION; BIOLOGICAL MARKERS; CHLOROFORM; DAPHNIA; ESTROGENS; GENE REGULATION; GOLD; MESSENGER-RNA; ATRAZINE
OSTI ID:
22133450
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0166-445X; CODEN: AQTODG; Other: PII: S0166-445X(10)00423-6; TRN: NL11R0745088493
Availability:
Available from http://dx.doi.org/10.1016/j.aquatox.2010.11.006
Submitting Site:
NLN
Size:
page(s) 351-357
Announcement Date:
Sep 26, 2013

Citation Formats

Hannas, Bethany R., Wang, Ying H., Thomson, Susanne, Kwon, Gwijun, Hong, Li, and LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu. Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna). Netherlands: N. p., 2011. Web. doi:10.1016/J.AQUATOX.2010.11.006.
Hannas, Bethany R., Wang, Ying H., Thomson, Susanne, Kwon, Gwijun, Hong, Li, & LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu. Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna). Netherlands. doi:10.1016/J.AQUATOX.2010.11.006.
Hannas, Bethany R., Wang, Ying H., Thomson, Susanne, Kwon, Gwijun, Hong, Li, and LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu. 2011. "Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna)." Netherlands. doi:10.1016/J.AQUATOX.2010.11.006. https://www.osti.gov/servlets/purl/10.1016/J.AQUATOX.2010.11.006.
@misc{etde_22133450,
title = {Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna)}
author = {Hannas, Bethany R., Wang, Ying H., Thomson, Susanne, Kwon, Gwijun, Hong, Li, and LeBlanc, Gerald A., E-mail: Gerald_LeBlanc@ncsu.edu}
abstractNote = {The induction of vitellogenin in oviparous vertebrates has become the gold standard biomarker of exposure to estrogenic chemicals in the environment. This biomarker of estrogen exposure also has been used in arthropods, however, little is known of the factors that regulate the expression of vitellogenin in these organisms. We investigated changes in accumulation of mRNA products of the vitellogenin gene Vtg2 in daphnids (Daphnia magna) exposed to a diverse array of chemicals. We further evaluated the involvement of hormonal factors in the regulation of vitellogenin expression that may be targets of xenobiotic chemicals. Expression of the Vtg2 gene was highly responsive to exposure to various chemicals with an expression range spanning approximately four orders of magnitude. Chemicals causing the greatest induction were piperonyl butoxide, chlordane, 4-nonylphenol, cadmium, and chloroform. Among these, only 4-nonylphenol is recognized to be estrogenic. Exposure to several chemicals also suppressed Vtg2 mRNA levels, as much as 100-fold. Suppressive chemicals included cyproterone acetate, acetone, triclosan, and atrazine. Exposure to the estrogens diethylstilbestrol and bisphenol A had little effect on vitellogenin mRNA levels further substantiating that these genes are not induced by estrogen exposure. Exposure to the potent ecdysteroids 20-hydroxyecdysone and ponasterone A revealed that Vtg2 was subject to strong suppressive control by these hormones. Vtg2 mRNA levels were not significantly affected from exposure to several juvenoid hormones. Results indicate that ecdysteroids are suppressors of vitellogenin gene expression and that vitellogenin mRNA levels can be elevated or suppressed in daphnids by xenobiotics that elicit antiecdysteroidal or ecdysteroidal activity, respectively. Importantly, daphnid Vtg2 is not elevated in response to estrogenic activity.}
doi = {10.1016/J.AQUATOX.2010.11.006}
journal = {Aquatic Toxicology}
issue = {2}
volume = {101}
journal type = {AC}
place = {Netherlands}
year = {2011}
month = {Jan}
}