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Optical Density Control of Autoradiographs After Photographic Processing (Preliminary Results); Controle de la Densite Optique des Autoradiogrammes Apres Developpement Photographique. (Resultats Preliminaires); Regulirovanie opticheskoj plotnosti avtoradiogramm posle fotoobrabotki; Control de la Densidad Optica de las Autorradiografias Despues del Tratamiento Fotografico. (Resultados Preliminares)

Conference:

Abstract

In many applications of photographic emulsions it would be desirable to control the optical density after the photographic processing. This would be useful for instance in certain problems of photographic dosimetry and particularly in many applications of autoradiography in medicine and biology. It would then be possible to recover information lost by overexposure as it frequently occurs unavoidably particularly in cases where important grain density variations are observed in the same autoradiograph specimen. The authors report the first results of an attempt to control the grain density after development and fixation of the photographic material by attacking the processed emulsion with diluted solutions of trypsin. By changing concentrations and temperature the attack can be made to proceed at any desirable speed so the trypsin solution acts like an ultra microtome removing very thin layers of the photographic emulsion. More important, the process could be followed continuously under the microscope by using a suitable chamber. Results are presented which suggest that this technique can give a rough idea of the incorporation with time of the radionuclide in the biological structure under observation. (author) [French] Dans diverses applications d'emulsions photographiques, il serait souhaitable de controler la densite optique apres avoir procede au  More>>
Authors:
Baptista, A. M.; [1]  Terrinha, A. [2] 
  1. Comissao de Estudos de Energia Nuclear, Instituto de Alta Cultura, Lisbon (Portugal)
  2. Instituto Portugues de Oncologia, Lisbon (Portugal)
Publication Date:
Oct 15, 1965
Product Type:
Conference
Report Number:
IAEA-SM-61/77
Resource Relation:
Conference: Symposium on Radioisotope Sample Measurement Techniques in Medicine and Biology, Vienna (Austria), 24-28 May 1965; Other Information: 4 refs., 3 figs.; Related Information: In: Radioisotope Sample Measurement Techniques in Medicine and Biology. Proceedings of the Symposium on Radioisotope Sample Measurement Techniques| 741 p.
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AUTORADIOGRAPHY; BIOLOGY; DOSIMETRY; GRAIN DENSITY; IMAGES; MEDICINE; MICROSCOPES; OPACITY; PHOTOGRAPHIC EMULSIONS; PHOTOGRAPHY; TRYPSIN
OSTI ID:
22131303
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M2503086427
Submitting Site:
INIS
Size:
page(s) 547-552
Announcement Date:
Sep 19, 2013

Conference:

Citation Formats

Baptista, A. M., and Terrinha, A. Optical Density Control of Autoradiographs After Photographic Processing (Preliminary Results); Controle de la Densite Optique des Autoradiogrammes Apres Developpement Photographique. (Resultats Preliminaires); Regulirovanie opticheskoj plotnosti avtoradiogramm posle fotoobrabotki; Control de la Densidad Optica de las Autorradiografias Despues del Tratamiento Fotografico. (Resultados Preliminares). IAEA: N. p., 1965. Web.
Baptista, A. M., & Terrinha, A. Optical Density Control of Autoradiographs After Photographic Processing (Preliminary Results); Controle de la Densite Optique des Autoradiogrammes Apres Developpement Photographique. (Resultats Preliminaires); Regulirovanie opticheskoj plotnosti avtoradiogramm posle fotoobrabotki; Control de la Densidad Optica de las Autorradiografias Despues del Tratamiento Fotografico. (Resultados Preliminares). IAEA.
Baptista, A. M., and Terrinha, A. 1965. "Optical Density Control of Autoradiographs After Photographic Processing (Preliminary Results); Controle de la Densite Optique des Autoradiogrammes Apres Developpement Photographique. (Resultats Preliminaires); Regulirovanie opticheskoj plotnosti avtoradiogramm posle fotoobrabotki; Control de la Densidad Optica de las Autorradiografias Despues del Tratamiento Fotografico. (Resultados Preliminares)." IAEA.
@misc{etde_22131303,
title = {Optical Density Control of Autoradiographs After Photographic Processing (Preliminary Results); Controle de la Densite Optique des Autoradiogrammes Apres Developpement Photographique. (Resultats Preliminaires); Regulirovanie opticheskoj plotnosti avtoradiogramm posle fotoobrabotki; Control de la Densidad Optica de las Autorradiografias Despues del Tratamiento Fotografico. (Resultados Preliminares)}
author = {Baptista, A. M., and Terrinha, A.}
abstractNote = {In many applications of photographic emulsions it would be desirable to control the optical density after the photographic processing. This would be useful for instance in certain problems of photographic dosimetry and particularly in many applications of autoradiography in medicine and biology. It would then be possible to recover information lost by overexposure as it frequently occurs unavoidably particularly in cases where important grain density variations are observed in the same autoradiograph specimen. The authors report the first results of an attempt to control the grain density after development and fixation of the photographic material by attacking the processed emulsion with diluted solutions of trypsin. By changing concentrations and temperature the attack can be made to proceed at any desirable speed so the trypsin solution acts like an ultra microtome removing very thin layers of the photographic emulsion. More important, the process could be followed continuously under the microscope by using a suitable chamber. Results are presented which suggest that this technique can give a rough idea of the incorporation with time of the radionuclide in the biological structure under observation. (author) [French] Dans diverses applications d'emulsions photographiques, il serait souhaitable de controler la densite optique apres avoir procede au developpement. Cette methode serait utile, par exemple pour resoudre certains problemes de dosimetrie photographique, et notamment dans maintes applications medicales et biologiques de l'autoradiographie. Il serait alors possible de recuperer les indications perdues en raison d'une surexposition, souvent inevitable, surtout dans les cas ou la densite du grain varie tres sensiblement dans un meme specimen autoradiographique. Les auteurs signalent les premiers resultats qu'ils ont pu obtenir en cherchant a controler la densite du grain, apres developpement et fixation de la substance photographique, en attaquant l'emulsion traitee avec des solutions diluees de tripsine. En modifiant les concentrations et les temperatures, il est possible de regler a volonte la vitesse de l'operation, de sorte que la solution de tripsine agit comme un ultramicrotome qui enleve des couches tres minces de l'emulsion photographique. Ce qui est plus important encore, les auteurs ont pu suivre le processus d1 une maniere continue sous le microscope en utilisant une chambre appropriee. Les resultats presentes permettent de penser qu'en recourant a cette technique, on pourrait se faire une idee approximative du progres dans le temps de l'incorporation du radionucleide dans la structure biologique consideree. (author) [Spanish] En muchos casos en que se emplean emulsiones fotograficas, conviene controlar la densidad optica despues del tratamiento fotografico. Ello es particularmente util, por ejemplo, en ciertos trabajos de dosimetria fotografica y sobre todo en numerosas aplicaciones de la autorradiografia en medicina y biologia. De esta manera seria posible recuperar datos perdidos por sobreexposicion, pues con frecuencia se observ an importantes variaciones de la densidad del grano en la misma muestra autorradiografica. En la presente memoria se presentan los primeros resultados obtenidos al controlar la densidad del grano- despues de revelar y fijar el material fotografico- por ataque de la emulsion mediante soluciones diluidas de tripsina. Variando la concentracion y la temperatura, se puede elegir la velocidad de ataque mas conveniente de modo que la solucion de tripsina actua como un ultramicrotomo, separando capas muy finas de la emulsion fotografica. Esta tecnica ha permitido tambien, cosa muy importante, seguir el proceso de modo continuo bajo el microscopio siempre que se utilice una camara adecuada. Los resultados presentados sugieren que esta tecnica permite formarse una idea aproximada de la manera en que los radionuclidos se incorporan en la estructura biologica sometida a observacion. (author) [Russian] Vo mnogih- sluchajah primenenija fotojemul'sij celesoobrazno regulirovat' opticheskuju plotnost' posle fotograficheskoj obrabotki. Jeto sleduet delat', naprimer, pri izuchenii opredelennyh problem fotograficheskoj dozimetrii, i osobenno vo mnogih sluchajah primenenija avtoradiografii v medicine i biologii. Pri jetom mozhno poluchit' informaciju, nesmotrja na perederzhku, kotoraja neizbezhno proishodit v nekotoryh sluchajah, v chastnosti, kogda v odnih i teh zhe avtoradiograficheskih obrazcah nabljudajutsja vazhnye variacii plotnosti zeren. Soobshhaetsja o pervyh rezul'tatah popytki regulirovat' plotnost' zerna posle projavlenija i zakreplenija fotograficheskogo materiala putem korrodirovanija obrabotannoj jemul'sii s pomoshh'ju razbavlennyh rastvorov tripsina. Izmenjaja koncentracii i temperaturu, mozhno regulirovat' po zhelaniju skorost' processa korrozii, i takim obrazom rastvor tripsina dejstvuet v kachestve ul'tramikrotoma, udaljaja tonchajshie sloi fotograficheskoj jemul'sii. Bolee togo, jetot process mozhno nabljudat' pod mikroskopom s pomosh'ju podhodjashej kameoy. Privodjatsja rezul'taty, kotorye govorjat o tom, chto s pomoshh'ju jetogo metoda mozhno sostavit' priblizitel'noe predstavlenie o vvedenii so vremenem radioizotopa v nabljudaemuju biologicheskuju strukturu. (author)}
place = {IAEA}
year = {1965}
month = {Oct}
}