You need JavaScript to view this

Relationship Between Hepatic Albumin and Sulphate Synthesis and its Use in Measurement of the Absolute Rate of Synthesis of Liver-Produced Plasma Proteins

Conference:

Abstract

A model is proposed whereby serum albumin synthesis is expressed in terms of production of inorganic sulphate in the liver and the entire organism, following the administration of {sup 35}S-L-cystine. The basis assumption involved is that the precursor amino acid pool for albumin synthesis in the liver is either identical with that of inorganic sulphate synthesis or that the two pools concerned are in rapid equilibrium with each other so that they can be treated as a single pool. The feasibility of the proposed model was tested by comparing the synthesis rate of rat serum albumin with the catabolic rate of the radioiodinated protein measured in the same animal. A good agreement between the two rates was noted in a group of adult rats, whereas an excess of anabolism was noted in young growing animals. In rats fed low-protein diet, the synthesis rate exceeded the catabolic rate; both being subnormal. The equilibrium between hepatic and plasma radiosulphate concentration was complete within four hours following the injection of {sup 35}S-cystine. The total radiosulphate production could then be evaluated after such an interval from the urinary excretion and serum concentration multiplied by the volume of the sulphate space. Lack of significant re-utilization  More>>
Authors:
Awwad, H. K.; Sheraki, A. S.; [1]  Radioisotope Unit, Medical Research Institute, Alexandria (Egypt)]
  1. Department of Radiology and Radiological Sciences, Cancer Institute, University of Cairo, Cairo (Egypt)
Publication Date:
Feb 15, 1971
Product Type:
Conference
Report Number:
IAEA-SM-136/4
Resource Relation:
Conference: Symposium on Dynamics Studies with Radioisotopes in Clinical Medicine and Research, Rotterdam (Netherlands), 31 Aug - 4 Sep 1970; Other Information: 10 refs., 3 tabs., 7 figs.; Related Information: In: Dynamic Studies with Radioisotopes in Medicine. Proceedings of the Symposium on Dynamics Studies with Radioisotopes in Clinical Medicine and Research| 924 p.
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; ALBUMINS; ANABOLISM; BLOOD PLASMA; CONCENTRATION RATIO; CYSTINE; LIVER; RATS; SELENIUM; SELENIUM 75; SULFATES; SULFUR 35; SYNTHESIS
OSTI ID:
22127319
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M2732082398
Submitting Site:
INIS
Size:
page(s) 69-81
Announcement Date:
Sep 12, 2013

Conference:

Citation Formats

Awwad, H. K., Sheraki, A. S., and Radioisotope Unit, Medical Research Institute, Alexandria (Egypt)]. Relationship Between Hepatic Albumin and Sulphate Synthesis and its Use in Measurement of the Absolute Rate of Synthesis of Liver-Produced Plasma Proteins. IAEA: N. p., 1971. Web.
Awwad, H. K., Sheraki, A. S., & Radioisotope Unit, Medical Research Institute, Alexandria (Egypt)]. Relationship Between Hepatic Albumin and Sulphate Synthesis and its Use in Measurement of the Absolute Rate of Synthesis of Liver-Produced Plasma Proteins. IAEA.
Awwad, H. K., Sheraki, A. S., and Radioisotope Unit, Medical Research Institute, Alexandria (Egypt)]. 1971. "Relationship Between Hepatic Albumin and Sulphate Synthesis and its Use in Measurement of the Absolute Rate of Synthesis of Liver-Produced Plasma Proteins." IAEA.
@misc{etde_22127319,
title = {Relationship Between Hepatic Albumin and Sulphate Synthesis and its Use in Measurement of the Absolute Rate of Synthesis of Liver-Produced Plasma Proteins}
author = {Awwad, H. K., Sheraki, A. S., and Radioisotope Unit, Medical Research Institute, Alexandria (Egypt)]}
abstractNote = {A model is proposed whereby serum albumin synthesis is expressed in terms of production of inorganic sulphate in the liver and the entire organism, following the administration of {sup 35}S-L-cystine. The basis assumption involved is that the precursor amino acid pool for albumin synthesis in the liver is either identical with that of inorganic sulphate synthesis or that the two pools concerned are in rapid equilibrium with each other so that they can be treated as a single pool. The feasibility of the proposed model was tested by comparing the synthesis rate of rat serum albumin with the catabolic rate of the radioiodinated protein measured in the same animal. A good agreement between the two rates was noted in a group of adult rats, whereas an excess of anabolism was noted in young growing animals. In rats fed low-protein diet, the synthesis rate exceeded the catabolic rate; both being subnormal. The equilibrium between hepatic and plasma radiosulphate concentration was complete within four hours following the injection of {sup 35}S-cystine. The total radiosulphate production could then be evaluated after such an interval from the urinary excretion and serum concentration multiplied by the volume of the sulphate space. Lack of significant re-utilization was demonstrated following the injection of radiosulphate. This is a decided advantage of the proposed method. However, extensive re-utilization of selenate selenium in the synthesis of the seleno-analogues of sulphur-amino acids was shown. This could explain the poor yield of radioselenate following the injection of {sup 75}Se-selenocystine and precludes the use of the latter agent as a tracer for measurement of synthesis of plasma proteins. (author)}
place = {IAEA}
year = {1971}
month = {Feb}
}