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Influence of Radioprotectors on Regeneration of the Mucosa of the Small Intestine of the Mouse after X-Irradiation

Abstract

Intestinal death occurs in mice from the third to the fifth day after doses of 1 to 10 krad and is preceded by inhibition of cell division, destruction of the crypts, shortening of the villi and denudation of the intestinal epithelium, BALB/c mice, some of which were protected with 2-{beta}-aminoethylisothiourea (AET) or with one of the following mixtures of chemical protectors: AET+ 5-hydroxytryptamine (5-HT), AET + 5-HT + glutathione (GSH), and AET + 5- HT + GSH + cysteine, were irradiated with doses of 1350 or 2000 R of X-rays (300 kV, 20 m A , dose rate 100 R/min). Mitotic activity, abnormal anaphases, size of nuclei, pyknosis and number of nuclei, migration of nuclei along the villi and incorporation of thymidine were investigated. In all experiments, lesions appeared later and were less pronounced in the protected than in the non-protected mice. Furthermore, AET and the associations of radioprotectors appear to protect the crypt cells of the small intestine during all phases of the mitotic cycle. The best results were obtained with a mixture of AET + 5-HT + GSH + cysteine. After such treatment and a dose of 2000 R of X-rays, a delay of 30 days is nevertheless  More>>
Authors:
Maisin, J. R. [1] 
  1. Radiobiological Department, Nuclear Energy Study Centre, Mol (Belgium)
Publication Date:
Aug 15, 1968
Product Type:
Conference
Report Number:
IAEA-SM-103/4
Resource Relation:
Conference: Symposium on the Effects of Radiation on Cellular Proliferation and Differentiation, Monaco (Monaco), 1-5 Apr 1968; Other Information: 12 refs., 14 figs., 2 tabs.; Related Information: In: Effects of Radiation on Cellular Proliferation and Differentiation. Proceedings of a Symposium on the Effects of Radiation on Cellular Proliferation and Differentiation| 586 p.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CELL DIVISION; CRYPT CELLS; CYSTEINE; DOSE RATES; EPITHELIUM; GLUTATHIONE; IRRADIATION; MICE; MIXTURES; REGENERATION; SMALL INTESTINE; THYMIDINE; X RADIATION
OSTI ID:
22121070
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
English
Contract Number:
Grant IAEA 347/R2/RB
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M0864077163
Submitting Site:
INIS
Size:
page(s) 541-556
Announcement Date:
Aug 19, 2013

Citation Formats

Maisin, J. R. Influence of Radioprotectors on Regeneration of the Mucosa of the Small Intestine of the Mouse after X-Irradiation. IAEA: N. p., 1968. Web.
Maisin, J. R. Influence of Radioprotectors on Regeneration of the Mucosa of the Small Intestine of the Mouse after X-Irradiation. IAEA.
Maisin, J. R. 1968. "Influence of Radioprotectors on Regeneration of the Mucosa of the Small Intestine of the Mouse after X-Irradiation." IAEA.
@misc{etde_22121070,
title = {Influence of Radioprotectors on Regeneration of the Mucosa of the Small Intestine of the Mouse after X-Irradiation}
author = {Maisin, J. R.}
abstractNote = {Intestinal death occurs in mice from the third to the fifth day after doses of 1 to 10 krad and is preceded by inhibition of cell division, destruction of the crypts, shortening of the villi and denudation of the intestinal epithelium, BALB/c mice, some of which were protected with 2-{beta}-aminoethylisothiourea (AET) or with one of the following mixtures of chemical protectors: AET+ 5-hydroxytryptamine (5-HT), AET + 5-HT + glutathione (GSH), and AET + 5- HT + GSH + cysteine, were irradiated with doses of 1350 or 2000 R of X-rays (300 kV, 20 m A , dose rate 100 R/min). Mitotic activity, abnormal anaphases, size of nuclei, pyknosis and number of nuclei, migration of nuclei along the villi and incorporation of thymidine were investigated. In all experiments, lesions appeared later and were less pronounced in the protected than in the non-protected mice. Furthermore, AET and the associations of radioprotectors appear to protect the crypt cells of the small intestine during all phases of the mitotic cycle. The best results were obtained with a mixture of AET + 5-HT + GSH + cysteine. After such treatment and a dose of 2000 R of X-rays, a delay of 30 days is nevertheless needed to allow complete recovery of the duodenal mucosa. The diminution of incorporation of thymidine and the prolongation of the regeneration time are symptoms of a 'biochemical shock', and the importance of this in the mechanism of action of sulphydryl compounds and of mixtures of protective drugs is discussed. (author)}
place = {IAEA}
year = {1968}
month = {Aug}
}