Abstract
Autoradiographs of bean (Phaseolus vulgaris L.) and alligatorweed (Alternanthera philoxeroides (Mart.) Griseb.) indicated that {sup 14}C-nitrile-labelled dichlobenil (2,6-dichlorobenzonitrile) was slightly absorbed by the leaf and some translocation occurred following foliar application. Plants with roots submersed in radioactive aqueous solution absorbed and translocated the {sup 14}C throughout the plant. An investigation of some of the chemical and physical properties of {sup 14}C-nitrile-labelled dichlobenil was conducted. Loss because of volatilization from counting planchets was considerably reduced by application of acrylic plastic immediately after the solution dried. The plastic coating also eliminated contamination of counting chambers and windows. Two higher plants (bean and alligatorweed ) and four fungi (Fusarium sp., Geotrichum sp., Penicillium sp., Trichoderma sp.) were selected for metabolism studies. Dichlobenil- {sup 14}C was added to Hoagland and Arnon's nutrient solution containing beans or alligatorweed and to liquid cultures containing the other organisms for 12 to 120 h. Extracts from the plants or fungi were chromatographed on silica gel thin-layers. Autoradiographs of the thin-layer chromatographed aqueous extracts revealed a {sup C}-labelled compound of Rf 0.25 that differed from that of dichlobenil, which was 0.6. After esterification of the extracts, a {sup 14}C-labelled compound was observed at Rf 0.95 on thin-layer chromatograms. Chromatography
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Pate, D. A.;
Funderburk, Jr., H. H.
[1]
- Auburn University Agricultural Experiment Station, Auburn, AL (United States)
Citation Formats
Pate, D. A., and Funderburk, Jr., H. H.
Absorption, Translocation and Metabolism of {sup 14}C-Labelled Dichlobenil.
IAEA: N. p.,
1966.
Web.
Pate, D. A., & Funderburk, Jr., H. H.
Absorption, Translocation and Metabolism of {sup 14}C-Labelled Dichlobenil.
IAEA.
Pate, D. A., and Funderburk, Jr., H. H.
1966.
"Absorption, Translocation and Metabolism of {sup 14}C-Labelled Dichlobenil."
IAEA.
@misc{etde_22118929,
title = {Absorption, Translocation and Metabolism of {sup 14}C-Labelled Dichlobenil}
author = {Pate, D. A., and Funderburk, Jr., H. H.}
abstractNote = {Autoradiographs of bean (Phaseolus vulgaris L.) and alligatorweed (Alternanthera philoxeroides (Mart.) Griseb.) indicated that {sup 14}C-nitrile-labelled dichlobenil (2,6-dichlorobenzonitrile) was slightly absorbed by the leaf and some translocation occurred following foliar application. Plants with roots submersed in radioactive aqueous solution absorbed and translocated the {sup 14}C throughout the plant. An investigation of some of the chemical and physical properties of {sup 14}C-nitrile-labelled dichlobenil was conducted. Loss because of volatilization from counting planchets was considerably reduced by application of acrylic plastic immediately after the solution dried. The plastic coating also eliminated contamination of counting chambers and windows. Two higher plants (bean and alligatorweed ) and four fungi (Fusarium sp., Geotrichum sp., Penicillium sp., Trichoderma sp.) were selected for metabolism studies. Dichlobenil- {sup 14}C was added to Hoagland and Arnon's nutrient solution containing beans or alligatorweed and to liquid cultures containing the other organisms for 12 to 120 h. Extracts from the plants or fungi were chromatographed on silica gel thin-layers. Autoradiographs of the thin-layer chromatographed aqueous extracts revealed a {sup C}-labelled compound of Rf 0.25 that differed from that of dichlobenil, which was 0.6. After esterification of the extracts, a {sup 14}C-labelled compound was observed at Rf 0.95 on thin-layer chromatograms. Chromatography of the unaltered extracts with 2,6- dichlorobenzoic acid revealed identical Rf-values. The esterified aqueous extracts chromatographed precisely with methyl-2,6-dichlorobenzoate. Gas chromatography of the {sup 14}C-labelled compound with an Rf of 0.95 exhibited a retention time identical to that of methyl-2,6-dichlorobenzoate. The quantity of {sup 14}C-labelled compound that chromatographed as 2,6-dichlorobenzoate increased with time of exposure of the various test organisms to dichlobenil {sup -14}C. Other metabolites of dichlobenil {sup -14}C. were not resolved by these chromatographic procedures. (author)}
place = {IAEA}
year = {1966}
month = {May}
}
title = {Absorption, Translocation and Metabolism of {sup 14}C-Labelled Dichlobenil}
author = {Pate, D. A., and Funderburk, Jr., H. H.}
abstractNote = {Autoradiographs of bean (Phaseolus vulgaris L.) and alligatorweed (Alternanthera philoxeroides (Mart.) Griseb.) indicated that {sup 14}C-nitrile-labelled dichlobenil (2,6-dichlorobenzonitrile) was slightly absorbed by the leaf and some translocation occurred following foliar application. Plants with roots submersed in radioactive aqueous solution absorbed and translocated the {sup 14}C throughout the plant. An investigation of some of the chemical and physical properties of {sup 14}C-nitrile-labelled dichlobenil was conducted. Loss because of volatilization from counting planchets was considerably reduced by application of acrylic plastic immediately after the solution dried. The plastic coating also eliminated contamination of counting chambers and windows. Two higher plants (bean and alligatorweed ) and four fungi (Fusarium sp., Geotrichum sp., Penicillium sp., Trichoderma sp.) were selected for metabolism studies. Dichlobenil- {sup 14}C was added to Hoagland and Arnon's nutrient solution containing beans or alligatorweed and to liquid cultures containing the other organisms for 12 to 120 h. Extracts from the plants or fungi were chromatographed on silica gel thin-layers. Autoradiographs of the thin-layer chromatographed aqueous extracts revealed a {sup C}-labelled compound of Rf 0.25 that differed from that of dichlobenil, which was 0.6. After esterification of the extracts, a {sup 14}C-labelled compound was observed at Rf 0.95 on thin-layer chromatograms. Chromatography of the unaltered extracts with 2,6- dichlorobenzoic acid revealed identical Rf-values. The esterified aqueous extracts chromatographed precisely with methyl-2,6-dichlorobenzoate. Gas chromatography of the {sup 14}C-labelled compound with an Rf of 0.95 exhibited a retention time identical to that of methyl-2,6-dichlorobenzoate. The quantity of {sup 14}C-labelled compound that chromatographed as 2,6-dichlorobenzoate increased with time of exposure of the various test organisms to dichlobenil {sup -14}C. Other metabolites of dichlobenil {sup -14}C. were not resolved by these chromatographic procedures. (author)}
place = {IAEA}
year = {1966}
month = {May}
}