You need JavaScript to view this

Studies on the Mechanism of Radiation Resistance in Micrococcus Radiodurans and its Sensitization

Conference:

Abstract

Efficient and accurate repair of radiation-induced lesions in M. radiodurans was investigated as to the cause of its extreme radioresistance. The cells were made permeable to deoxyribonucleoside triphosphate by treatment with non-ionic detergent, Triton X-100. After irradiation with 2 krad gamma rays more than 80% of the single-strand scissions were rejoined in the permeable cells within 10 min at 37 Degree-Sign C. This fast repair process requires the presence of deoxyribonucleoside triphosphates and NAD. However, rejoining of DNA strand scission was incomplete after prolonged incubation in the permeable cells. This suggests that alternate repair reaction(s) is necessary for complete recovery. The other type of radiation lesion was found by postirradiation incubation at non-permissive temperature, which markedly sensitizes this bacterium to radiation. Postincubation at this temperature also sensitizes the cells to chemicals that damage DNA. The extreme radioresistance of this bacterium was also lost by mutation and an isolated radiosensitive mutant showed almost the same radiosensitivity as E. coli K12 or B/r. These results are discussed in connection with the extreme radioresistance of M. radiodurans. (author)
Authors:
Kitayama, S.; Matsuyama, A. [1] 
  1. Radiobiology Laboratory, Institute of Physical and Chemical Research, Wako-shi, Saitama-ken (Japan)
Publication Date:
Jun 15, 1978
Product Type:
Conference
Report Number:
IAEA-SM-221/38
Resource Relation:
Conference: International Symposium on Food Preservation by Irradiation, Wageningen (Netherlands), 21-25 Nov 1977; Other Information: 10 refs., 3 tabs., 7 figs.; Related Information: In: Food Preservation by Irradiation. Vol. II. Proceedings of an International Symposium on Food Preservation by Irradiation| 446 p.
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL RADIATION EFFECTS; DAMAGE; DETERGENTS; DNA; GAMMA RADIATION; INCUBATION; MICROCOCCUS RADIODURANS; MUTANTS; MUTATIONS; NAD; RADICIDATION; RADIOSENSITIVITY; REPAIR
OSTI ID:
22113910
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy); World Health Organization, Geneva (Switzerland)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; ISBN 92-0-010378-2; TRN: XA13M1796070892
Submitting Site:
INIS
Size:
page(s) 145-156
Announcement Date:
Jul 20, 2013

Conference:

Citation Formats

Kitayama, S., and Matsuyama, A. Studies on the Mechanism of Radiation Resistance in Micrococcus Radiodurans and its Sensitization. IAEA: N. p., 1978. Web.
Kitayama, S., & Matsuyama, A. Studies on the Mechanism of Radiation Resistance in Micrococcus Radiodurans and its Sensitization. IAEA.
Kitayama, S., and Matsuyama, A. 1978. "Studies on the Mechanism of Radiation Resistance in Micrococcus Radiodurans and its Sensitization." IAEA.
@misc{etde_22113910,
title = {Studies on the Mechanism of Radiation Resistance in Micrococcus Radiodurans and its Sensitization}
author = {Kitayama, S., and Matsuyama, A.}
abstractNote = {Efficient and accurate repair of radiation-induced lesions in M. radiodurans was investigated as to the cause of its extreme radioresistance. The cells were made permeable to deoxyribonucleoside triphosphate by treatment with non-ionic detergent, Triton X-100. After irradiation with 2 krad gamma rays more than 80% of the single-strand scissions were rejoined in the permeable cells within 10 min at 37 Degree-Sign C. This fast repair process requires the presence of deoxyribonucleoside triphosphates and NAD. However, rejoining of DNA strand scission was incomplete after prolonged incubation in the permeable cells. This suggests that alternate repair reaction(s) is necessary for complete recovery. The other type of radiation lesion was found by postirradiation incubation at non-permissive temperature, which markedly sensitizes this bacterium to radiation. Postincubation at this temperature also sensitizes the cells to chemicals that damage DNA. The extreme radioresistance of this bacterium was also lost by mutation and an isolated radiosensitive mutant showed almost the same radiosensitivity as E. coli K12 or B/r. These results are discussed in connection with the extreme radioresistance of M. radiodurans. (author)}
place = {IAEA}
year = {1978}
month = {Jun}
}