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Quantitative Aspects of Sulphur Metabolism in the Ruminant

Conference:

Abstract

In a series of four experiments {sup 35}S sulphate was given intra-ruminally to lactating dairy goats at feeding times (twice daily) during periods of 10 to 11 d. Rumen contents were sampled on the 8th, 9th and 10th day of {sup 35}S application. Radioactivity was determined in the volatile sulphur fraction (sulphide-sulphur) and in protein-bound sulphur (fraction insoluble in trichloroacetic acid). The ratio between the specific activities of protein sulphur and of sulphide sulphur was considered to be an estimate for that fraction of sulphur-containing amino acids present in the proteins of rumen contents, which was synthesized by microorganisms. This interpretation is based on the assumption that sulphide is the most important source of inorganic sulphur for the synthesis of sulphur amino acids and that incorporation of inorganic sulphur compounds of a a higher state of oxidation into organic molecules may quantitatively be neglected. In three experiments the animals received rations containing 16% to 17% crude protein. In these experiments the ratio of specific activities of protein and sulphide sulphur was found to vary from 0.32 to 0.41. According to the assumptions mentioned above, this would indicate that 32 to 41% of the total amount of protein-bound sulphur amino acids  More>>
Authors:
Landis, J. [1] 
  1. Institut fuer Tierernaehrung, Eidgenoessische Technische Hochschule, Zurich (Switzerland)
Publication Date:
Jul 01, 1968
Product Type:
Conference
Report Number:
IAEA-SM-97/24
Resource Relation:
Conference: Symposium on the use of isotope in studies of nitrogen metabolism in the soils-plant-animal system, Vienna (Austria), 28 Aug - 1 Sep 1967; Other Information: 2 refs., 5 tabs., 3 figs.; Related Information: In: Isotope Studies on the Nitrogen Chain. Proceedings of the Symposium on the Use of Isotopes in Studies of Nitrogen Metabolism in the Soil-Plant-Animal System| 360 p.
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACIDS; CHLORINATED ALIPHATIC HYDROCARBONS; GOATS; LACTATES; METABOLISM; MICROORGANISMS; OXIDATION; PROTEINS; SULFATES; SULFIDES; SULFUR; SULFUR 35; SYNTHESIS; UREA; VOLATILITY
OSTI ID:
22108088
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M1072065899
Submitting Site:
INIS
Size:
page(s) 221-228
Announcement Date:
Jun 27, 2013

Conference:

Citation Formats

Landis, J. Quantitative Aspects of Sulphur Metabolism in the Ruminant. IAEA: N. p., 1968. Web.
Landis, J. Quantitative Aspects of Sulphur Metabolism in the Ruminant. IAEA.
Landis, J. 1968. "Quantitative Aspects of Sulphur Metabolism in the Ruminant." IAEA.
@misc{etde_22108088,
title = {Quantitative Aspects of Sulphur Metabolism in the Ruminant}
author = {Landis, J.}
abstractNote = {In a series of four experiments {sup 35}S sulphate was given intra-ruminally to lactating dairy goats at feeding times (twice daily) during periods of 10 to 11 d. Rumen contents were sampled on the 8th, 9th and 10th day of {sup 35}S application. Radioactivity was determined in the volatile sulphur fraction (sulphide-sulphur) and in protein-bound sulphur (fraction insoluble in trichloroacetic acid). The ratio between the specific activities of protein sulphur and of sulphide sulphur was considered to be an estimate for that fraction of sulphur-containing amino acids present in the proteins of rumen contents, which was synthesized by microorganisms. This interpretation is based on the assumption that sulphide is the most important source of inorganic sulphur for the synthesis of sulphur amino acids and that incorporation of inorganic sulphur compounds of a a higher state of oxidation into organic molecules may quantitatively be neglected. In three experiments the animals received rations containing 16% to 17% crude protein. In these experiments the ratio of specific activities of protein and sulphide sulphur was found to vary from 0.32 to 0.41. According to the assumptions mentioned above, this would indicate that 32 to 41% of the total amount of protein-bound sulphur amino acids were synthesized by microorganisms from inorganic (sulphide) sulphur. In the fourth experiment the animal received a ration containing only 10% protein but supplemented with urea to bring the total crude protein content to 13%. In this experiment the ratio of specific activities was 0.70, indicating a much higher contribution of microbial synthesis of sulphur amino acids than in the experiments with a more abundant protein supply. The time course of the specific activity of protein-bound-sulphur in the milk is taken as a reference base for an interpretation of labelling of body proteins in terms of turnover rates.}
place = {IAEA}
year = {1968}
month = {Jul}
}