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The Use Of {sup 15}N in the Study of Nitrogen Uptake and Metabolism in Plants; Primenenie azota-15 pri izuchenii vzaimodejstviya azotnykh udobrenij s pochvoj i usvoeniya ikh rasteniyami

Conference:

Abstract

Some forty years ago Mattson attempted to represent soil solutions as ionic states. Later on, he further developed his theory with the aid of the latest achievements in physical chemistry. In 1955 Schoffield applied chemical thermodynamics to make the interrelations between the solid and liquid phases of the soil even more precise. Nitrogen occupies a special position among the plant nutrients. The greatest success in nitrogen uptake and metabolism studies, however, has been achieved only recently after the development of isotope techniques. The study of nitrogen metabolism using isotope techniques has been carried out for some years at the N. Poushkarov Institute of Soil Science using optical methods of isotope detection. Certain of the results obtained recently point to the great opportunities offered by the use of the optical method. Greenhouse and field experiments were carried out with wheat, oats and lucerne. Ammonium sulphate with 11.50 at.%, {sup 15}N,andurea 5.55 at % were used as sources of nitrogen. Depending on the conditions, the nitrogen introduced with fertilizers was utilized by the plants in amounts ranging from 47 to 56% in the greenhouses, and from 38 to 45% m the field. It was established that the soil was the source of  More>>
Authors:
Gyrbuchev, I.; Dinchev, D. [1] 
  1. Institut Pochvovedenija Im.N. Pushkarova, Sofija (Bulgaria)
Publication Date:
Jul 01, 1968
Product Type:
Conference
Report Number:
IAEA-SM-97/34
Resource Relation:
Conference: Symposium on the use of isotope in studies of nitrogen metabolism in the soils-plant-animal system, Vienna (Austria), 28 Aug - 1 Sep 1967; Other Information: 23 refs., 9 tabs., 3 figs.; Related Information: In: Isotope Studies on the Nitrogen Chain. Proceedings of the Symposium on the Use of Isotopes in Studies of Nitrogen Metabolism in the Soil-Plant-Animal System| 360 p.
Subject:
60 APPLIED LIFE SCIENCES; ALANINES; ALBUMINS; AMMONIUM SULFATES; ARGININE; CHLOROPHYLL; DETECTION; FERTILIZERS; GLOBULINS; GLYCINE; HISTIDINE; LYSINE; METABOLISM; NITROGEN; NITROGEN 15; NUTRIENTS; OATS; SERINE; SOILS; THREONINE; WHEAT
OSTI ID:
22108077
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy); Joint Commission on Applied Radioactivity of the International Council of Scientific Unions, Paris (France)
Country of Origin:
IAEA
Language:
Russian
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13M1060065888
Submitting Site:
INIS
Size:
page(s) 63-77
Announcement Date:
Jun 27, 2013

Conference:

Citation Formats

Gyrbuchev, I., and Dinchev, D. The Use Of {sup 15}N in the Study of Nitrogen Uptake and Metabolism in Plants; Primenenie azota-15 pri izuchenii vzaimodejstviya azotnykh udobrenij s pochvoj i usvoeniya ikh rasteniyami. IAEA: N. p., 1968. Web.
Gyrbuchev, I., & Dinchev, D. The Use Of {sup 15}N in the Study of Nitrogen Uptake and Metabolism in Plants; Primenenie azota-15 pri izuchenii vzaimodejstviya azotnykh udobrenij s pochvoj i usvoeniya ikh rasteniyami. IAEA.
Gyrbuchev, I., and Dinchev, D. 1968. "The Use Of {sup 15}N in the Study of Nitrogen Uptake and Metabolism in Plants; Primenenie azota-15 pri izuchenii vzaimodejstviya azotnykh udobrenij s pochvoj i usvoeniya ikh rasteniyami." IAEA.
@misc{etde_22108077,
title = {The Use Of {sup 15}N in the Study of Nitrogen Uptake and Metabolism in Plants; Primenenie azota-15 pri izuchenii vzaimodejstviya azotnykh udobrenij s pochvoj i usvoeniya ikh rasteniyami}
author = {Gyrbuchev, I., and Dinchev, D.}
abstractNote = {Some forty years ago Mattson attempted to represent soil solutions as ionic states. Later on, he further developed his theory with the aid of the latest achievements in physical chemistry. In 1955 Schoffield applied chemical thermodynamics to make the interrelations between the solid and liquid phases of the soil even more precise. Nitrogen occupies a special position among the plant nutrients. The greatest success in nitrogen uptake and metabolism studies, however, has been achieved only recently after the development of isotope techniques. The study of nitrogen metabolism using isotope techniques has been carried out for some years at the N. Poushkarov Institute of Soil Science using optical methods of isotope detection. Certain of the results obtained recently point to the great opportunities offered by the use of the optical method. Greenhouse and field experiments were carried out with wheat, oats and lucerne. Ammonium sulphate with 11.50 at.%, {sup 15}N,andurea 5.55 at % were used as sources of nitrogen. Depending on the conditions, the nitrogen introduced with fertilizers was utilized by the plants in amounts ranging from 47 to 56% in the greenhouses, and from 38 to 45% m the field. It was established that the soil was the source of nearly half the nitrogen of the plants. Fertilized plants took up more of the soil nitrogen than the unfertilized plants. The nitrogen introduced into the soil was found in all fractions of the plants after 24 h and was in the non-protein organic nitrogen, constitution proteins, chlorophyll and reserve proteins of the plants. The highest amounts of {sup 15}N were found in the following free amino acids: arginine, histidine, lysine and the amide aspargine. In the bound amino acids, alanine, threonine, serine and glycine were highest in {sup 15}N. Phosphorus application increased the amounts of nitrogen in the amino acids. It was established that nitrogen turnover was greatest in chlorophyll and the constitution proteins. In the study of the quality of wheat grains, it has been shown that the highest amounts of nitrogen were found in the glutenins, followed by the fractions of residual nitrogen, prolamins and the water-soluble nitrogen compounds. The least amounts were found in the albumins and globulins. (author) [Russian] 40 let nazad Mattson pytalsja predstavit' pochvennye rastvory kak ionnye sostojanija. Pozdnee on razvil svoju teoriju, osnovyvajas' na poslednih dostizhenijah v oblasti fizicheskoj himii. V 1955 godu Shhoffild primenil himicheskuju termodinamiku dlja togo, chtoby eshhe bol'she utochnit' vzaimootnoshenija mezhdu tverdymi i zhidkimi fazami pochvy. Azot zanimaet osoboe mesto sredi pitatel'nyh veshhestv rastenij. Odnako naibol'shij uspeh v issledovanijah azotistogo pogloshhenija i metabolizma byl dostignut lish' nedavno posle razrabotki izotopnyh metodov. Issledovanie azotistogo metabolizma s pomoshh'ju izotopnyh metodov provodilos' v techenie neskol'kih let v Institute pochvovedenija im. N.Pushkarova s ispol'zovaniem opticheskih metodov obnaruzhenija izotopov. Nekotorye iz poluchennyh nedavno rezul'tatov ukazyvajut na ogromnye vozmozhnosti, kotorye otkryvajutsja v rezul'tate ispol'zovanija opticheskogo metoda. Jeksperimenty v teplichnyh i polevyh uslovijah provodilis' na pshenice, ovse i ljucerne. V kachestve istochnikov azota ispol'zovalis' sul'fat ammonija s 11,50 i mochevina s 5,55 atomnyh procentov {sup 15}N. V zavisimosti ot uslovij azot, vvodimyj s udobrenijami, ispol'zovalsja rastenijami v kolichestve ot 47 do 56% v teplicah i ot 38 do 45% - v polevyh uslovijah. Ustanovleno, chto pochva javljalas' istochnikom pochti poloviny azota v rastenijah. Udobrennye rastenija pogloshhali bol'she pochvennogo azota, chem neudobrennye. Azot, vvedennyj v pochvu, byl obnaruzhen vo vseh frakcijah rastenij spustja 24 chasa i soderzhalsja v nebelkovom organicheskom azote, konstitutivnyh belkah, hlorofille i rezervnyh belkah rastenij. Naibol'shee kolichestvo 15N bylo obnaruzheno v sledujushhih svobodnyh aminokislotah: arginine, gistidine, lizine i amidoasparagine. V svjazannyh aminokislotah - alanine, treonine, serine i glicine - soderzhalos' naibol'shee kolichestvo 15N. Primenenie fosfora uvelichivalo kolichestvo azota v aminokislotah. Ustanovleno, chto oborot azota byl samym bol'shim v hlorofille i konstitutivnyh belkah. Pri issledovanii kachestva pshenichnyh zeren bylo ustanovleno, chto naibol'shee kolichestvo azota bylo obnaruzheno v gljutenine, zatem vo frakcijah ostatochnogo azota, prolamina i vodorastvorimyh azotnyh soedinenijah. Naimen'shee kolichestvo bylo obnaruzheno v albumine i globuline.}
place = {IAEA}
year = {1968}
month = {Jul}
}