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Tyrosine Metabolism in the Blowfly, Calliphora Erythrocephala; Metabolisme de la tyrosine dans la Calliphora Erythrocephala; Metabolizm tirozina u myasnoj mukhi, Calliphora Erythrocephala; Metabolismo de la tirosina en la mosca Calliphora Erythrocephala

Conference:

Abstract

Sclerotization, i.e. the transfoimation of the soft, white larval cuticle into the hardened, dark puparium, is due to interaction of o-quinones with the cuticle proteins. Using radioactively labelled amino-acids, it has been shown that N-acetyldopamine is the immediate precursor of the sclerotizing quinones in Calliphora erythrocephala. The intermediate steps in the biosynthesis of N-acetyldopamine are hydroxylation of tyrosine to dopa, decarboxylation of dopa to dopamine and N-acetylation of dopamine to N-acetyldopamine. This metabolic pathway of tyrosine is followed only in the final-instar larvae; early third-instar larvae catabolize tyrosine by transamination to p-hydroxyphenylpyruvic acid and reduction to p-hydroxyphenyllactic and -propionic acid. The metabolic shift from transamination to hydroxylation and decarboxylation is brought about by the hormone of the prothoracic gland, ecdysone, and can be inhibited by ligation or destruction of the ring gland. Injection of ecdysone into the ligated animals leads within 10-14 h toactivationofthedopadecaiboxylase, presumably as the result of biosynthesis of enzyme protein. The action of the hormone is not a direct one on the biosynthetic mechanism, but indirect, the first action of the hormone being on the chromosomes (puffing phenomenon). The working hypothesis is that the hormone interacting with the genetic material leads to stimulation of the synthesis of  More>>
Authors:
Sekeris, C. E. [1] 
  1. Physiological Chemistry Institute, University of Munich, Munich (Germany)
Publication Date:
Sep 15, 1963
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests, Athens (Greece), 22-26 Apr 1963; Other Information: 4 figs., 12 refs.; Related Information: In: Radiation and Radioisotopes Applied to Insects of Agricultural Importance. Proceedings of the Symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests| 526 p.
Subject:
60 APPLIED LIFE SCIENCES; ACETYLATION; BIOLOGICAL PATHWAYS; BIOSYNTHESIS; CYTOPLASM; DECARBOXYLATION; DOPA; DOPAMINE; ENZYMES; FLIES; HORMONES; HYDROXYLATION; LABELLING; LARVAE; MESSENGER-RNA; METABOLISM; PROPIONIC ACID; QUINONES; TRACER TECHNIQUES; TYROSINE
OSTI ID:
22100732
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13R0292058780
Submitting Site:
INIS
Size:
page(s) 281-287
Announcement Date:
Jun 06, 2013

Conference:

Citation Formats

Sekeris, C. E. Tyrosine Metabolism in the Blowfly, Calliphora Erythrocephala; Metabolisme de la tyrosine dans la Calliphora Erythrocephala; Metabolizm tirozina u myasnoj mukhi, Calliphora Erythrocephala; Metabolismo de la tirosina en la mosca Calliphora Erythrocephala. IAEA: N. p., 1963. Web.
Sekeris, C. E. Tyrosine Metabolism in the Blowfly, Calliphora Erythrocephala; Metabolisme de la tyrosine dans la Calliphora Erythrocephala; Metabolizm tirozina u myasnoj mukhi, Calliphora Erythrocephala; Metabolismo de la tirosina en la mosca Calliphora Erythrocephala. IAEA.
Sekeris, C. E. 1963. "Tyrosine Metabolism in the Blowfly, Calliphora Erythrocephala; Metabolisme de la tyrosine dans la Calliphora Erythrocephala; Metabolizm tirozina u myasnoj mukhi, Calliphora Erythrocephala; Metabolismo de la tirosina en la mosca Calliphora Erythrocephala." IAEA.
@misc{etde_22100732,
title = {Tyrosine Metabolism in the Blowfly, Calliphora Erythrocephala; Metabolisme de la tyrosine dans la Calliphora Erythrocephala; Metabolizm tirozina u myasnoj mukhi, Calliphora Erythrocephala; Metabolismo de la tirosina en la mosca Calliphora Erythrocephala}
author = {Sekeris, C. E.}
abstractNote = {Sclerotization, i.e. the transfoimation of the soft, white larval cuticle into the hardened, dark puparium, is due to interaction of o-quinones with the cuticle proteins. Using radioactively labelled amino-acids, it has been shown that N-acetyldopamine is the immediate precursor of the sclerotizing quinones in Calliphora erythrocephala. The intermediate steps in the biosynthesis of N-acetyldopamine are hydroxylation of tyrosine to dopa, decarboxylation of dopa to dopamine and N-acetylation of dopamine to N-acetyldopamine. This metabolic pathway of tyrosine is followed only in the final-instar larvae; early third-instar larvae catabolize tyrosine by transamination to p-hydroxyphenylpyruvic acid and reduction to p-hydroxyphenyllactic and -propionic acid. The metabolic shift from transamination to hydroxylation and decarboxylation is brought about by the hormone of the prothoracic gland, ecdysone, and can be inhibited by ligation or destruction of the ring gland. Injection of ecdysone into the ligated animals leads within 10-14 h toactivationofthedopadecaiboxylase, presumably as the result of biosynthesis of enzyme protein. The action of the hormone is not a direct one on the biosynthetic mechanism, but indirect, the first action of the hormone being on the chromosomes (puffing phenomenon). The working hypothesis is that the hormone interacting with the genetic material leads to stimulation of the synthesis of specific (messenger-) RNA which is transferred to the cytoplasm and produces enzyme proteins. (author) [French] L'induration, c'est-a-dire la transformation de la cuticule larvaire, douce et blanche, en un puparium dur et sombre, est due a l'interaction d'ortho-quinones avec les proteines de la cuticule. Grace a l'emploi d'acides amines marques au moyen de substances radioactives, on a montre que la N-acetyl-dopamine est le precurseur immediat des quinones produisant l'induration dans la Calliphora erythrocephala. Les stades intermediaires de la biosynthese de la N-acetyl-dopamine sont l'hydroxylation de la tyrosine, qui se transforme en dioxyphenylalamine (dopa), la decarboxylation de la dopa en dopamine et la N-acetylation de la dopamine en N-acetyl-dopamine. Ce processus metabolique de la tyrosine ne se produit qu'au dernier stade de l'etat larvaire; les trois premiers stades produisent le catabolisme de la tyrosine par transamination en acide p-hydroxyphenyl-pyruvique et reduction en acide p-hydroxyphenyl- lactique et propionique. Le processus metabolique de la transamination a l'hydroxylation est provoque par l'hormone de la glande prothoracique, l'ecdyson, et peut etre rendu impossible par la ligature ou l'ablation de la glande annulaire. L'injection d'ecdyson aux animaux ligatures entraine, au bout de 10-14 h, l'activation de la decarboxylase de la dopa, due vraisembleblement a la bioxynthese de la proteine de l'enzyme. L'action de l'hormone sur le mecanisme de biosynthese est indirecte, le premier effet de l'hormone etant d'agir sur les chromosomes (phenomene de gonflage). L'hypothese de travail est que l'hormone qui entre en interaction avec la matiere genetique a pour effet de stimuler la synthese de l'ARN specifique (vecteur) qui est transmis au cytoplasme et produit les proteines d'enzjmnes. (author) [Spanish] La esclerotizacion (transformacion de la cuticula blanda y blanca de la larva en la cubierta dura y oscura de la ninfa) se debe a la accion reciproca de o-quinonas con las proteinas de la cuticula. Empleando aminoacidos marcados se ha comprobado que la N-acetildopamina es el precursor inmediato de las qui nonas esclera tizantes en la Calliphora erythrocephala. Las etapas intermedias en la biosintesis de la N-acetildopamina son la hidroxilacion de la tirosina que da dioxifenil-alanina, la descarboxilacion de esta que da dopamina, y la N-acetilacion de la dopamina que da la N-acetildopamina. Este proceso metabolico de la tirosina se produce solamente en las larvas del ultimo estadio; al principio del tercer estadio las larvas catabolizan tirosina por transaminacion, dando acido p-hidroxifenilpinivico, y por reduccion, dando los acidos p-hidroxifenillactico y p-hidroxifenil propionico. Esta transicion metabolica desde la transaminacion a la hidroxilacion y descarboxilacion se debe a la hormona de la glandula protoracica (ecdyson) y se puede inhibir ligando o destruyendo la glandula anular. Si se inyecta ecdyson en los animales ligados se produce al cabo de 10 a 14 h una activacion de la dopadecarboxilasa, debida probablemente a la biosintesis de proteinas enzimaticas. La accion de la hormona sobre el mecanismo de biosintesis no es directa sino indirecta, dejandose sentir sus primeros efectos sobre los cromosomas (fenomeno de turgencia). La hipotesis de que se partio es que la hormona que actua sobre las sustancias geneticas y que sufre la accion de estas estimula la sintesis de un ARN especifico (vehiculo) que se traslada al citoplasma y produce proteinas enzimaticas. (author) [Russian] Sklerotizatsiya, t.e. prevrashchenie myagkoj beloj kutikuly lichinki v zatverdevshij temnyj puparij, proiskhodit blagodarya vzaimodejstviyu ortokhinonov s belkami kutikuly. S pomoshch'yu mechennykh izotopami aminokislot bylo pokazano, chto N-atsetildopamin yavlyaetsya blizhajshim predshestvennikom sklerotiziruitsikh khinonov u Calliphora erythrocephala. Promezhutochnymi stadiyami v biosinteze N-atsetildopamina yavlyayutsya gidroksilirova- nie tirozina v dopa, dekarboksilirovanie dopa do dopamina i N-atsetilirovanie dopamina do N-atsetildopamina. Ehtot metabolicheskij protsess prevrashcheniya tirozina proiskhodit tol'ko u lichinok na poslednej stadii razvitiya. U lichinok v rannej faze tret'ej stadii proiskhodit katabolizatsiya ti rozina putem pereaminirovaniya v para-oksifenilpirovikogradnuzeh kislotu i vosstanovlenie v paraoksifenilmolochnuyu para-oksifenilpropionovuyu kislotu. Metabolicheskoe smeshchenie ot pereaminirovaniya do gidroksilizatsii i dekarboksilizatsii vyzyvaetsya gormonom perednegrudnoj zhelezy,ehkdisonom, i mozhet zaderzhivat'sya nalozheniem ligatury i razrusheniem kol'tsevoj zhelezy. Vvedenie ehkdisona zhivotnym s nalozhennoj ligaturoj privodit v techenie 10 - 14 chasov k aktivatsii dopadekarboksilazy, veroyatno v rezul'tate biosinteza fermentativnogo belka. Gormon okazyvaet ne pryamoe, a kosvennoe dejstvie na mekhanizm biosinteza i, prezhde vsego, na khromosomy (yavlenie otechnosti). Rabochaya gipoteza zaklyuchaetsya v tom, chto gormon, vzaimodejstvuya s geneticheskim materialom, privodit k stimulirovaniyu sinteza osoboj (perenosnoj) RNK, kotoraya perenositsya v kletochnuyu protoplazmu i proizvodit fermentativnye belki. (author)}
place = {IAEA}
year = {1963}
month = {Sep}
}