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Use of Isotopes for Investigating the Behaviour and Ecology of Insect Pests in Some Recent Studies; Emploi des radioisotopes dans des recherches recentes sur le comportement et l'ecologie des insectes nuisibles; Ispol'zovanie radioizotopov dlya issledovaniya povedeniya i ehkologii nasekomykh-vreditelej v Irane; Empleo de isotopos en estudios recientes sobre el comportamiento y la ecologia de las plagas insectiles

Conference:

Abstract

Investigations into the ecology, behaviour, dispersal and longevity of insects have always provided challenges to the entomologist. The use of isotopes is an effective tool and the following is a report on some interesting problems solved by their application. Anopheles stephensi is the main vector o f malaria in southern Iran. On P{sup 32}-labelled mosquitoes, the dispersal, behaviour, digestion of blood meal, maturation of ovaries and length of gonotrophic cycles were successfully worked out. It was found that in about 80% of the cases the mosquito needed two blood meals for the completion of the first cycle. The first cycle itself was completed in 4-5 d depending upon the temperature. Labelled mosquitoes which had emerged overnight were released in an isolated village. The ratio of active mosquitoes to total catch was worked out every day and thus, on the assumption that the natural population remained constant, the mortality rate, which was found to be exponential for the first six days, was worked out. The mating behaviour of the female was also studied by using normal females which had mated once with P{sup 32}-labelled males. It was found that the female mates more than once and that after mating with an  More>>
Authors:
Quraishi Sayeed, M. [1] 
  1. Cento Institute of Nuclear Science, Teheran (Iran, Islamic Republic of)
Publication Date:
Sep 15, 1963
Product Type:
Conference
Resource Relation:
Conference: Symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests, Athens (Greece), 22-26 Apr 1963; Other Information: 8 refs.; Related Information: In: Radiation and Radioisotopes Applied to Insects of Agricultural Importance. Proceedings of the Symposium on the Use and Application of Radioisotopes and Radiation in the Control of Plant and Animal Insect Pests| 526 p.
Subject:
60 APPLIED LIFE SCIENCES; BEHAVIOR; BLOOD; FEEDING; FEMALES; GLUCOSE; LARVAE; MALARIA; MALES; MATING; MATURATION; MORTALITY; MOSQUITOES; OVARIES; PHOSPHORUS 32; WHEAT
OSTI ID:
22100716
Research Organizations:
International Atomic Energy Agency, Vienna (Austria); Food and Agriculture Organization of the United Nations, Rome (Italy)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA13R0276058764
Submitting Site:
INIS
Size:
page(s) 93-99
Announcement Date:
Jun 06, 2013

Conference:

Citation Formats

Quraishi Sayeed, M. Use of Isotopes for Investigating the Behaviour and Ecology of Insect Pests in Some Recent Studies; Emploi des radioisotopes dans des recherches recentes sur le comportement et l'ecologie des insectes nuisibles; Ispol'zovanie radioizotopov dlya issledovaniya povedeniya i ehkologii nasekomykh-vreditelej v Irane; Empleo de isotopos en estudios recientes sobre el comportamiento y la ecologia de las plagas insectiles. IAEA: N. p., 1963. Web.
Quraishi Sayeed, M. Use of Isotopes for Investigating the Behaviour and Ecology of Insect Pests in Some Recent Studies; Emploi des radioisotopes dans des recherches recentes sur le comportement et l'ecologie des insectes nuisibles; Ispol'zovanie radioizotopov dlya issledovaniya povedeniya i ehkologii nasekomykh-vreditelej v Irane; Empleo de isotopos en estudios recientes sobre el comportamiento y la ecologia de las plagas insectiles. IAEA.
Quraishi Sayeed, M. 1963. "Use of Isotopes for Investigating the Behaviour and Ecology of Insect Pests in Some Recent Studies; Emploi des radioisotopes dans des recherches recentes sur le comportement et l'ecologie des insectes nuisibles; Ispol'zovanie radioizotopov dlya issledovaniya povedeniya i ehkologii nasekomykh-vreditelej v Irane; Empleo de isotopos en estudios recientes sobre el comportamiento y la ecologia de las plagas insectiles." IAEA.
@misc{etde_22100716,
title = {Use of Isotopes for Investigating the Behaviour and Ecology of Insect Pests in Some Recent Studies; Emploi des radioisotopes dans des recherches recentes sur le comportement et l'ecologie des insectes nuisibles; Ispol'zovanie radioizotopov dlya issledovaniya povedeniya i ehkologii nasekomykh-vreditelej v Irane; Empleo de isotopos en estudios recientes sobre el comportamiento y la ecologia de las plagas insectiles}
author = {Quraishi Sayeed, M.}
abstractNote = {Investigations into the ecology, behaviour, dispersal and longevity of insects have always provided challenges to the entomologist. The use of isotopes is an effective tool and the following is a report on some interesting problems solved by their application. Anopheles stephensi is the main vector o f malaria in southern Iran. On P{sup 32}-labelled mosquitoes, the dispersal, behaviour, digestion of blood meal, maturation of ovaries and length of gonotrophic cycles were successfully worked out. It was found that in about 80% of the cases the mosquito needed two blood meals for the completion of the first cycle. The first cycle itself was completed in 4-5 d depending upon the temperature. Labelled mosquitoes which had emerged overnight were released in an isolated village. The ratio of active mosquitoes to total catch was worked out every day and thus, on the assumption that the natural population remained constant, the mortality rate, which was found to be exponential for the first six days, was worked out. The mating behaviour of the female was also studied by using normal females which had mated once with P{sup 32}-labelled males. It was found that the female mates more than once and that after mating with an active male, the spermathead became active. Counts of up to twice the background (12 counts/min) were obtained by using males giving about 15 000 counts/min. Studies of the injection of saliva in glucose solution during feeding were also made on P{sup 32}-labelled mosquitoes. Eurygaster integriceps is a serious pest of wheat in Iran, Pakistan and the Middle East. Using P{sup 32} activated wheat plants, the feeding behaviour of the first-instar nymph was investigated. Other foods, radioactively labelled, were also studied and it was found that feeding was essential for the first moult, even if the food consisted of water absorbed in a filter paper. (author) [French] Les recherches sur l'ecologie, le comportement, la dispersion et la longevite des insectes ont toujours pose des problemes interessants aux entomologistes. Les radioisotopes constituent un outil efficace, et l'auteur expose les resultats auxquels il est parvenu grace a leur emploi dans l'etude de certains de ces problemes. L'Anopheles stephensi est le principal vecteur du paludisme en Iran meridional. En marquant des moustiques avec {sup 32}P, on a pu determiner la dispersion, le comportement, la digestion du sang, le developpement des ovaires et la duree des cycles gonotrophiques. On a constate que, dans environ 80% des cas, le moustique a besoin de deux repas de sang pour realiser le premier cycle. La duree de ce cycle a ete de 4-5 j selon la temperature. Les moustiques marques qui etaient parvenus a l'etat adulte pendant la nuit ont ete laches sur un village isole. On a calcule tous les jours le pourcentage de moustiques irradies par rapport a la prise totale; en partant de l'hypothese que la population naturelle demeurait constante, on a determine le taux de mortalite et constate que c 'etait une fonction exponentielle pour les six premiers jours. On a egalement etudie le comportement sexuel de la femelle a l'aide de femelles normales qui s'etaient accouplees une fois avec des males marques avec {sup 32}P. On a pu etablir que la femelle s'accouple plus d' une fois et que la spermatotheque devient radioactive apres accouplement avec un male irradie. On a enregiste un nombre de coups pouvant atteindre jusqu'a deux fois le bruit de fond (12 cpm) en utilisant des males donnant environ 15 000 cpm. Avec des moustiques marques avec {sup 32}P, on a egalement etudie l'injection de salive dans une solution de'glucose pendant l'absorption de nourriture. L'Eurygaster integriceps est un insecte tres nuisible au ble en Iran, au Pakistan et au Moyen-Orient. Au moyen de grains marques par {sup 32}P, on a etudie le comportement alimentaire au premier stade nymphal, On a egalement etudie d'autres nourritures marquees et on a constate que la nymphe devait se nourrir, ne serait-ce que d'eau absorbee par un filtre en papier, pour franchir le premier stade de son evolution. (author) [Spanish] Las investigaciones sobre la ecologia, el comportamiento, la distribucion y la longevidad de los insectos suelen ofrecer a los entomologos problemas de dificil solucion. Los isotopos han resultado auxiliares muy eficaces para estas investigaciones; en la memoria se informa sobre algunos problemas interesantes que se han podido resolver gracias a su empleo. El Anopheles stephensi es el principal vector paludico en el sur del Iran. Empleando mosquitos marcados con {sup 32}P se pudo determinar su distribucion, su comportamiento, la digestion de la sangre, la maduracion de los ovarios y la longitud de los ciclos gonotroficos. Se comprobo que en un 80 por ciento de los casos el mosquito necesita alimentarse dos veces con sangre para completar el primer ciclo, cuya duracion es de 4 a 5 dias segun la temperatura. A los mosquitos marcados que surgian de la ninfa durante la noche se los liberaba en una aldea aislada. Diariamente se calculaba el porcentaje de anofeles capturados que estaban marcados y asi, suponiendo que el numero die mosquitos de la zona permanencia constante, se pudo determinar el indice de mortalidad, que resulto ser exponencial durante los seis primeros dias. Tambien se estudio la copulacion de las hembras utilizando hembras normales Que habian copulado una vez con machos marcados con {sup 32}P. Se pudo observar que las hembras copulan mas de una vez y despues de hacerlo con un macho activado sus espermatotecas se hacian tambien radiactivas. Con machos que daban hasta 15 000 impulsos/min se obtuvieron cifras de recuento de hasta el doble del fondo parasitario (12 impulsos/min). Con mosquitos marcados con {sup 32}P se hicieron estudios sobre la inyeccion de saliva en una solucion de glucosa durante el proceso de alimentacion. El Eurygaster integriceps es una plaga muy peligrosa del trigo en el Iran, el Pakistan y el Oriente Medio. El autor investigo el modo de alimentarse de las ninfas del primer estadio utilizando plantas de trigo activadas con {sup 32}P. Tambien se estudiaron otros alimentos marcados radiactivamente y se encontro que la alimentacion es esencial para la primera muda, aunque consiste solamente en agua embebida en un papel de filtro. (author) [Russian] Issledovaniya ehkologii, povedeniya, rasprostraneniya i sroka zhizni nasekomykh vsegda privlekali ehntomologov. Ispol'zovanie radioizotopov yavlyaetsya ehffektivnym orudiem issledovaniya; nizhe daetsya soobshchenie otnositel'no neskol'kikh interesnykh problem, razreshennykh s ikh pomoshch'yu. Anopheles Stephensi yavlyaetsya glavnym perenoschikom malyarii v yuzhnom Irane. Blagodarya ispol'zovaniyu mechennykh P{sup 32} komarov byli polucheny svedeniya otnositel'no ikh rasprostraneniya, povedeniya, usvoeniya imi krovi, sozrevaniya yaichnikov i prodolzhitel'nosti gonotroficheskikh tsiklov. Bylo ustanovleno, chto prblizitel'ko v 80% sluchaev dlya zaversheniya pervogo tsikla komaram trebovalos' dvazhdy krovyanoe pitanie. Sam pervyj tsikl zavershalsya v zavisimosti ot temperatury za chetyre ili pyat' sutok. Vyplodivshiesya za noch' mechenye komary vypuskalis' na svobodu v izolirovannoj derevne. Sootnoshenie mezhdu aktivnymi komarami i obshchim ikh ulovom ustavlivalos' kazhdyj den' i, pri uslovii neizmennosti prirodnoj populyatsii, opredelyalas' smertnost', prichem okazalos', chto ona v techenie pervykh shesti sutok yavlyalas' ehksponentnoj. Bylo izucheno takzhe brachnoe povedenie samok putem ispol'zovaniya normal'nykh samok, sparivavshikhsya v proshlom odin raz s mechennymi P{sup 32} samtsami. Bylo ustanovleno, chto samka sparivaetsya bolee odnogo raza i chto posle sparivaniya s aktivnym samtsom spermateka stanovitsya aktivnoj. Ispol'zuya samtsov, davavshikh 15 000 otschetov v minutu,byli polucheny schety, vdvoe prevyshavshie fonovye (12 schetov v minutu). Pri pomoshchi ispol'zovaniya mechennykh P{sup 32} komarov byli provedeny takzhe issledovaniya po in{sup e}ktsii slyuny v rastvor glyukozy vo vremya pitaniya. Eurygaster intergriceps yavlyaetsya opasnym vreditelem pshenitsy v Irane, Pakistane i na Srednem Vostoke. S pomoshch'yu pshenitsy, mechennoj P{sup 32}, byl izuchen rezhim pitaniya kukolki na ee pervovozrastnoj stadii. Byli takzhe izucheny drugie vidy pishchi, mechennoj radioaktivnymi veshchestvami, i obnaruzheno, chto pitanie neobkhodimo v period pervoj lin'ki, esli dazhe pishcha sostoit iz vody, vpitannoj fil'troval'noj bumagoj. (author)}
place = {IAEA}
year = {1963}
month = {Sep}
}