Abstract
Major research issues in oral protein delivery include the stabilization of protein in delivery devices which could increase its oral bioavailability. The study deals with development of oral insulin delivery system utilizing biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles and modifying its surface with Concanavalin A to increase lymphatic uptake. Surface-modified PLGA nanoparticles were characterized for conjugation efficiency of ligand, shape and surface morphology, particle size, zeta potential, polydispersity index, entrapment efficiency, and in vitro drug release. Stability of insulin in the developed formulation was confirmed by SDS-PAGE, and integrity of entrapped insulin was assessed using circular dichroism spectrum. Ex vivo study was performed on Wistar rats, which exhibited the higher intestinal uptake of Con A conjugated nanoparticles. In vivo study performed on streptozotocin-induced diabetic rats which indicate that a surface-modified nanoparticle reduces blood glucose level effectively within 4 h of its oral administration. In conclusion, the present work resulted in successful production of Con A NPs bearing insulin with sustained release profile, and better absorption and stability. The Con A NPs showed high insulin uptake, due to its relative high affinity for non-reducing carbohydrate residues i.e., fucose present on M cells and have the potential for oral insulin delivery in effective
More>>
Hurkat, Pooja;
Jain, Aviral;
Jain, Ashish;
Shilpi, Satish;
Gulbake, Arvind;
Jain, Sanjay K., E-mail: drskjainin@yahoo.com
[1]
- Dr. Hari Singh Gour Vishwavidyalaya, Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences (India)
Citation Formats
Hurkat, Pooja, Jain, Aviral, Jain, Ashish, Shilpi, Satish, Gulbake, Arvind, and Jain, Sanjay K., E-mail: drskjainin@yahoo.com.
Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery.
Netherlands: N. p.,
2012.
Web.
doi:10.1007/S11051-012-1219-4.
Hurkat, Pooja, Jain, Aviral, Jain, Ashish, Shilpi, Satish, Gulbake, Arvind, & Jain, Sanjay K., E-mail: drskjainin@yahoo.com.
Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery.
Netherlands.
https://doi.org/10.1007/S11051-012-1219-4
Hurkat, Pooja, Jain, Aviral, Jain, Ashish, Shilpi, Satish, Gulbake, Arvind, and Jain, Sanjay K., E-mail: drskjainin@yahoo.com.
2012.
"Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery."
Netherlands.
https://doi.org/10.1007/S11051-012-1219-4.
@misc{etde_22077332,
title = {Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery}
author = {Hurkat, Pooja, Jain, Aviral, Jain, Ashish, Shilpi, Satish, Gulbake, Arvind, and Jain, Sanjay K., E-mail: drskjainin@yahoo.com}
abstractNote = {Major research issues in oral protein delivery include the stabilization of protein in delivery devices which could increase its oral bioavailability. The study deals with development of oral insulin delivery system utilizing biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles and modifying its surface with Concanavalin A to increase lymphatic uptake. Surface-modified PLGA nanoparticles were characterized for conjugation efficiency of ligand, shape and surface morphology, particle size, zeta potential, polydispersity index, entrapment efficiency, and in vitro drug release. Stability of insulin in the developed formulation was confirmed by SDS-PAGE, and integrity of entrapped insulin was assessed using circular dichroism spectrum. Ex vivo study was performed on Wistar rats, which exhibited the higher intestinal uptake of Con A conjugated nanoparticles. In vivo study performed on streptozotocin-induced diabetic rats which indicate that a surface-modified nanoparticle reduces blood glucose level effectively within 4 h of its oral administration. In conclusion, the present work resulted in successful production of Con A NPs bearing insulin with sustained release profile, and better absorption and stability. The Con A NPs showed high insulin uptake, due to its relative high affinity for non-reducing carbohydrate residues i.e., fucose present on M cells and have the potential for oral insulin delivery in effective management of Type 1 diabetes condition.}
doi = {10.1007/S11051-012-1219-4}
journal = []
issue = {11}
volume = {14}
journal type = {AC}
place = {Netherlands}
year = {2012}
month = {Nov}
}
title = {Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery}
author = {Hurkat, Pooja, Jain, Aviral, Jain, Ashish, Shilpi, Satish, Gulbake, Arvind, and Jain, Sanjay K., E-mail: drskjainin@yahoo.com}
abstractNote = {Major research issues in oral protein delivery include the stabilization of protein in delivery devices which could increase its oral bioavailability. The study deals with development of oral insulin delivery system utilizing biodegradable poly(lactic-co-glycolic acid) (PLGA) nanoparticles and modifying its surface with Concanavalin A to increase lymphatic uptake. Surface-modified PLGA nanoparticles were characterized for conjugation efficiency of ligand, shape and surface morphology, particle size, zeta potential, polydispersity index, entrapment efficiency, and in vitro drug release. Stability of insulin in the developed formulation was confirmed by SDS-PAGE, and integrity of entrapped insulin was assessed using circular dichroism spectrum. Ex vivo study was performed on Wistar rats, which exhibited the higher intestinal uptake of Con A conjugated nanoparticles. In vivo study performed on streptozotocin-induced diabetic rats which indicate that a surface-modified nanoparticle reduces blood glucose level effectively within 4 h of its oral administration. In conclusion, the present work resulted in successful production of Con A NPs bearing insulin with sustained release profile, and better absorption and stability. The Con A NPs showed high insulin uptake, due to its relative high affinity for non-reducing carbohydrate residues i.e., fucose present on M cells and have the potential for oral insulin delivery in effective management of Type 1 diabetes condition.}
doi = {10.1007/S11051-012-1219-4}
journal = []
issue = {11}
volume = {14}
journal type = {AC}
place = {Netherlands}
year = {2012}
month = {Nov}
}