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Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization

Abstract

Considering for the potential application in tissue engineering, polyaniline (PANi) film was fabricated via a two-step route: a self-assembled monolayer of C{sub 6}H{sub 5}NHC{sub 3}H{sub 6}Si(OMe){sub 3} was firstly formed on the single-crystal Si substrate; the conducting PANi film was then prepared through electroless surface polymerization of the aniline molecules on the aniline monolayer-bearing silane surface in an acidic aqueous solution. The formation of PANi film on Si surface was confirmed by characterizations of X-ray photoelectron spectroscope (XPS) and specular reflectance Fourier transform infrared (SR-FTIR) spectrum, etc. At last, the proliferation behaviors of PC-12 cells on the PANi film surface were studied by the [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) colorimetric assays, acridine orange fluorometric staining, and scanning electron microscope (SEM) observation, etc. The results demonstrate that the as-prepared PANi film provides high ability for cell proliferation, exhibiting promising potentials as surface coating to cultivate neuronal cells for applications in the tissue engineering.
Authors:
Sheng, Liu; [1]  Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)]; Wang Jinqing, E-mail: jqwang@lzb.ac.cn; [1]  Dong, Zhang; Puliang, Zhang; [2]  Junfei, Ou; [1]  Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)]; Bin, Liu; [2]  Yang Shengrong, E-mail: sryang@lzb.ac.cn [1] 
  1. Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000 (China)
  2. School of Stomatology, Lanzhou University, Lanzhou 730000 (China)
Publication Date:
Mar 15, 2010
Product Type:
Journal Article
Resource Relation:
Journal Name: Applied Surface Science; Journal Volume: 256; Journal Issue: 11; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ACRIDINE ORANGE; ANILINE; BROMIDES; CELL PROLIFERATION; FILMS; FOURIER TRANSFORM SPECTROMETERS; INFRARED SPECTRA; MONOCRYSTALS; ORGANIC SILICON COMPOUNDS; POLYMERIZATION; PROLIFERATION; SCANNING ELECTRON MICROSCOPY; SILANES; SUBSTRATES; SURFACE COATING; SURFACES; TETRAZOLIUM; X-RAY PHOTOELECTRON SPECTROSCOPY
OSTI ID:
22057516
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0169-4332; CODEN: ASUSEE; Other: PII: S0169-4332(09)01755-3; TRN: NL10R0284017998
Availability:
Available from http://dx.doi.org/10.1016/j.apsusc.2009.12.046
Submitting Site:
NLN
Size:
page(s) 3427-3431
Announcement Date:
Feb 21, 2013

Citation Formats

Sheng, Liu, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Wang Jinqing, E-mail: jqwang@lzb.ac.cn, Dong, Zhang, Puliang, Zhang, Junfei, Ou, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Bin, Liu, and Yang Shengrong, E-mail: sryang@lzb.ac.cn. Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization. Netherlands: N. p., 2010. Web. doi:10.1016/J.APSUSC.2009.12.046.
Sheng, Liu, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Wang Jinqing, E-mail: jqwang@lzb.ac.cn, Dong, Zhang, Puliang, Zhang, Junfei, Ou, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Bin, Liu, & Yang Shengrong, E-mail: sryang@lzb.ac.cn. Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization. Netherlands. https://doi.org/10.1016/J.APSUSC.2009.12.046
Sheng, Liu, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Wang Jinqing, E-mail: jqwang@lzb.ac.cn, Dong, Zhang, Puliang, Zhang, Junfei, Ou, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Bin, Liu, and Yang Shengrong, E-mail: sryang@lzb.ac.cn. 2010. "Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization." Netherlands. https://doi.org/10.1016/J.APSUSC.2009.12.046.
@misc{etde_22057516,
title = {Investigation on cell biocompatible behaviors of polyaniline film fabricated via electroless surface polymerization}
author = {Sheng, Liu, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Wang Jinqing, E-mail: jqwang@lzb.ac.cn, Dong, Zhang, Puliang, Zhang, Junfei, Ou, Graduate School of Chinese Academy of Sciences, Beijing 100039 (China)], Bin, Liu, and Yang Shengrong, E-mail: sryang@lzb.ac.cn}
abstractNote = {Considering for the potential application in tissue engineering, polyaniline (PANi) film was fabricated via a two-step route: a self-assembled monolayer of C{sub 6}H{sub 5}NHC{sub 3}H{sub 6}Si(OMe){sub 3} was firstly formed on the single-crystal Si substrate; the conducting PANi film was then prepared through electroless surface polymerization of the aniline molecules on the aniline monolayer-bearing silane surface in an acidic aqueous solution. The formation of PANi film on Si surface was confirmed by characterizations of X-ray photoelectron spectroscope (XPS) and specular reflectance Fourier transform infrared (SR-FTIR) spectrum, etc. At last, the proliferation behaviors of PC-12 cells on the PANi film surface were studied by the [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) colorimetric assays, acridine orange fluorometric staining, and scanning electron microscope (SEM) observation, etc. The results demonstrate that the as-prepared PANi film provides high ability for cell proliferation, exhibiting promising potentials as surface coating to cultivate neuronal cells for applications in the tissue engineering.}
doi = {10.1016/J.APSUSC.2009.12.046}
journal = []
issue = {11}
volume = {256}
journal type = {AC}
place = {Netherlands}
year = {2010}
month = {Mar}
}