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Flight Muscle Development in the Males of Glossina Pallidipes Reared for the Sterile Insect Technique

Abstract

The project's main goal was to study the influence of laboratory conditions on the development of flight muscles and the ability to fly in males of Glossina pallidipes Austen. Flight muscles can serve as an important criterion in the quality control of mass reared tsetse flies. All experiments were performed in the research and training facility in Bratislava which provided the flies. The experiments were generally performed by comparing different age groups and groups with different flight activity. To acquire data, several approaches were employed, i.e. classical measurements (residual dry weight, thoracic surface) as well as other alternatives - flight mill, electron microscopy and immunohistochemistry - to visualize and analyse muscle development. The results clearly identified differences in age groups. Slight changes in the development of flight muscles regarding different chances to fly were also detected, but these were not sufficiently significant to decrease the quality of males produced in mass rearing facilities. No distinct trends (rising or declining of amount of metabolites) in the groups studied were detected. The differences were in the amount of analysed metabolic components and the structure of the flight muscles. Our results suggest that, similar to other Glossina species, in G. pallidipes males the  More>>
Authors:
Ciampor, F Jr; Palosova, Z; Mancosova, L; Takac, P [1] 
  1. Institute of Zoology, Slovak Academy of Sciences, Bratislava, SK-845 06 (Slovakia)
Publication Date:
Jul 15, 2012
Product Type:
Technical Report
Report Number:
IAEA-TECDOC-1683
Resource Relation:
Other Information: Figs., 2 tabs., 33 refs.; Related Information: In: Quality Control for Expanded Tsetse Production, Sterilization and Field Application| 149 p.
Subject:
60 APPLIED LIFE SCIENCES; AGE GROUPS; ELECTRON MICROSCOPY; GLOSSINA; MALES; MASS REARING; METABOLITES; MUSCLES; QUALITY CONTROL; STERILE MALE TECHNIQUE; SURFACES
OSTI ID:
22030333
Research Organizations:
International Atomic Energy Agency, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Insect Pest Control Section, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISBN 978-92-0-130110-9; ISSN 1011-4289; TRN: XA12R0307119145
Availability:
Available from INIS in electronic form. Also available on-line: http://www-pub.iaea.org/MTCD/publications/PDF/TE_1683_web.pdf; Enquiries should be addressed to IAEA, Marketing and Sales Unit, Publishing Section, E-mail: sales.publications@iaea.org; Web site: http://www.iaea.org/books
Submitting Site:
INIS
Size:
page(s) 83-96
Announcement Date:
Jan 29, 2013

Citation Formats

Ciampor, F Jr, Palosova, Z, Mancosova, L, and Takac, P. Flight Muscle Development in the Males of Glossina Pallidipes Reared for the Sterile Insect Technique. IAEA: N. p., 2012. Web.
Ciampor, F Jr, Palosova, Z, Mancosova, L, & Takac, P. Flight Muscle Development in the Males of Glossina Pallidipes Reared for the Sterile Insect Technique. IAEA.
Ciampor, F Jr, Palosova, Z, Mancosova, L, and Takac, P. 2012. "Flight Muscle Development in the Males of Glossina Pallidipes Reared for the Sterile Insect Technique." IAEA.
@misc{etde_22030333,
title = {Flight Muscle Development in the Males of Glossina Pallidipes Reared for the Sterile Insect Technique}
author = {Ciampor, F Jr, Palosova, Z, Mancosova, L, and Takac, P}
abstractNote = {The project's main goal was to study the influence of laboratory conditions on the development of flight muscles and the ability to fly in males of Glossina pallidipes Austen. Flight muscles can serve as an important criterion in the quality control of mass reared tsetse flies. All experiments were performed in the research and training facility in Bratislava which provided the flies. The experiments were generally performed by comparing different age groups and groups with different flight activity. To acquire data, several approaches were employed, i.e. classical measurements (residual dry weight, thoracic surface) as well as other alternatives - flight mill, electron microscopy and immunohistochemistry - to visualize and analyse muscle development. The results clearly identified differences in age groups. Slight changes in the development of flight muscles regarding different chances to fly were also detected, but these were not sufficiently significant to decrease the quality of males produced in mass rearing facilities. No distinct trends (rising or declining of amount of metabolites) in the groups studied were detected. The differences were in the amount of analysed metabolic components and the structure of the flight muscles. Our results suggest that, similar to other Glossina species, in G. pallidipes males the first days after emergence are crucial for successful muscle development. On the other hand, rearing in cages does not negatively influence the quality of males with respect to their ability to fly and actively search for females in the wild after release. We also compared the mating behaviour of irradiated and non-irradiated males. We initiated the development of a functional walk-in field cage in which to rear a small colony of G. pallidipes under semi-natural conditions. Our work suggested that outside climatic conditions and suitable cage components, e.g. food source, limit the successful realization of using such a cage for rearing tsetse flies. (author)}
place = {IAEA}
year = {2012}
month = {Jul}
}