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Direct Measurement of Tritium in Biological Materials with the Liquid Scintillation Counter; Determination quantitative directe du tritium dans les substances biologiques, au moyen de compteurs a scintillations a liquides; Neposredstvennoe izmerenie kolichestva tritiya v biologicheskikh materialakh pri pomoshchi zhidkogo stsintillyatsionnogo schetchika; Determinacion cuantitativa directa del tritio en sustancias biologicas mediante contadores de centelleador liquido

Conference:

Abstract

Procedures for measurement of tritium in animal tissues and isolated tissue fractions have been reported in the literature. In all the work reported, however, a liquid scintillation coincidence spectrometer has been used for the counting of the samples. In this work a single phototube liquid scintillation counter was used for the measurements. Investigation centred primarily on the direct dissolution of animal tissue in Hyamine 10-X and the suspension counting of lyophilized tissue in scintillating gels. Different tissues, such as liver, intestine, blood and skin, can be dissolved directly in Hyamine and measured with an efficiency of 10-15%. The techniques of quenching correction and certain phosphorescence phenomena as they affect the counting in a single phototube counting system are discussed. The sensitivity of the method, although somewhat less than that of the coincidence-counting arrangement, seems to be sufficient for many applications. The work reported was carried out on tissue samples from mice previously injected with tritium- labelled thymidine. When a dose of approximately 1 {mu}c tritiated thymidine per g of body weight was injected into the mice, the metabolic fate of the thymidine incorporated into deoxyribonucleic acid (DNA) of the various tissues could be followed for a period of one month.  More>>
Authors:
Halvorsen, K [1] 
  1. Institutt for Atomenergi, Kjeller, Lillestroem (Norway)
Publication Date:
Jan 15, 1962
Product Type:
Conference
Resource Relation:
Conference: Symposium on Tritium in the Physical and Biological Sciences, Vienna (Austria), 3-10 May 1961; Other Information: 4 figs., 3 tabs., 11 refs.; Related Information: In: Tritium in the Physical and Biological Sciences. V. 1. Proceedings of a Symposium| 380 p.
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; ANIMAL TISSUES; BLOOD; DNA; EFFICIENCY; INTESTINES; LIQUID SCINTILLATION DETECTORS; LIQUIDS; MICE; PHOSPHORESCENCE; SENSITIVITY; SKIN; SUSPENSIONS; THYMIDINE; TRITIUM
OSTI ID:
22028526
Research Organizations:
International Atomic Energy Agency, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ISSN 0074-1884; TRN: XA12R0278116792
Submitting Site:
INIS
Size:
page(s) 313-323
Announcement Date:
Jan 18, 2013

Conference:

Citation Formats

Halvorsen, K. Direct Measurement of Tritium in Biological Materials with the Liquid Scintillation Counter; Determination quantitative directe du tritium dans les substances biologiques, au moyen de compteurs a scintillations a liquides; Neposredstvennoe izmerenie kolichestva tritiya v biologicheskikh materialakh pri pomoshchi zhidkogo stsintillyatsionnogo schetchika; Determinacion cuantitativa directa del tritio en sustancias biologicas mediante contadores de centelleador liquido. IAEA: N. p., 1962. Web.
Halvorsen, K. Direct Measurement of Tritium in Biological Materials with the Liquid Scintillation Counter; Determination quantitative directe du tritium dans les substances biologiques, au moyen de compteurs a scintillations a liquides; Neposredstvennoe izmerenie kolichestva tritiya v biologicheskikh materialakh pri pomoshchi zhidkogo stsintillyatsionnogo schetchika; Determinacion cuantitativa directa del tritio en sustancias biologicas mediante contadores de centelleador liquido. IAEA.
Halvorsen, K. 1962. "Direct Measurement of Tritium in Biological Materials with the Liquid Scintillation Counter; Determination quantitative directe du tritium dans les substances biologiques, au moyen de compteurs a scintillations a liquides; Neposredstvennoe izmerenie kolichestva tritiya v biologicheskikh materialakh pri pomoshchi zhidkogo stsintillyatsionnogo schetchika; Determinacion cuantitativa directa del tritio en sustancias biologicas mediante contadores de centelleador liquido." IAEA.
@misc{etde_22028526,
title = {Direct Measurement of Tritium in Biological Materials with the Liquid Scintillation Counter; Determination quantitative directe du tritium dans les substances biologiques, au moyen de compteurs a scintillations a liquides; Neposredstvennoe izmerenie kolichestva tritiya v biologicheskikh materialakh pri pomoshchi zhidkogo stsintillyatsionnogo schetchika; Determinacion cuantitativa directa del tritio en sustancias biologicas mediante contadores de centelleador liquido}
author = {Halvorsen, K}
abstractNote = {Procedures for measurement of tritium in animal tissues and isolated tissue fractions have been reported in the literature. In all the work reported, however, a liquid scintillation coincidence spectrometer has been used for the counting of the samples. In this work a single phototube liquid scintillation counter was used for the measurements. Investigation centred primarily on the direct dissolution of animal tissue in Hyamine 10-X and the suspension counting of lyophilized tissue in scintillating gels. Different tissues, such as liver, intestine, blood and skin, can be dissolved directly in Hyamine and measured with an efficiency of 10-15%. The techniques of quenching correction and certain phosphorescence phenomena as they affect the counting in a single phototube counting system are discussed. The sensitivity of the method, although somewhat less than that of the coincidence-counting arrangement, seems to be sufficient for many applications. The work reported was carried out on tissue samples from mice previously injected with tritium- labelled thymidine. When a dose of approximately 1 {mu}c tritiated thymidine per g of body weight was injected into the mice, the metabolic fate of the thymidine incorporated into deoxyribonucleic acid (DNA) of the various tissues could be followed for a period of one month. (author) [French] Diverses techniques de determination du tritium dans les tissus animaux et fractions isolees de tissus animaux ont deja ete decrites. Mais toutes comportent l'emploi d'un spectrometre de coincidences a scintillations a liquides pour le comptage des echantillons. Pour faire cette determination, l'auteur s'est servi d'un seul phototube-compteur a scintillations a liquides. Il a etudie surtout la dissolution directe de tissu animal dans de l'hyamine 10-X, et le comptage de tissus lyophilises en suspension dans des gels a scintillations. Certains tissus, tels que le foie, l'intestin, le sang et la peau, peuvent Etre dissous directement dans l'hyamine et le rendement de la detection peut etre de 10 a 15%. L'auteur examine les techniques de correction de coupage et certains phenomenes de phosphorescence dans la mesure ou ils ont une influence sur le dispositif de comptage a un seul phototube. Cette methode, bien que moins precise que le dispositif de coincidence, l'est suffisamment pour de nombreuses applications. L'experience qui fait l'objet du memoire a porte sur des echantillons de tissus de souris auxquelles on avait injecte au prealable de la thymidine marquee au tritium. Lorsqu'une dose d'environ 1 {mu}c de thymidine tritiee par gramme de poids du corps est injectee aux souris, on peut suivre pendant un mois les transformations metaboliques de la thymidine incorporee a l'acide deoxyribonucleique (ADN) des divers tissus. (author) [Spanish] Ya se han presentado informes acerca de los procedimientos aplicados para la determinacion cuantitativa del tritio en tejidos animales y fracciones aisladas de los mismos. Sin embargo, en los trabajos respectivos se han utilizado siempre, para el recuento de la muestra, espectrometros de coincidencia de centelleador liquido. En cambio, en el presente trabajo las mediciones se han efectuado con un contador de centelleador liquido equipado con un fototubo unico. Se investigo principalmente la disolucion directa de tejido animal en la hiamina 10-KH y el recuento del tejido liofilizado en suspension en geles de centelleo. Es posible disolver directamente en hiamina tejidos diferentes tales como el hepatico, el intestinal, el sanguineo y el cutaneo, y medirlos con un rendimiento de 10 a 15%. El autor examina la tecnica aplicada para la correccion por extincion, asi como ciertos fenomenos de fosforescencia que afectan la determinacion en el sistema de recuento de fototubo unico. Parece que el metodo ofrece una sensibilidad suficiente para muchas aplicaciones, aunque un poco menor que la del dispositivo de recuento por coincidencias. La labor de que se informa se llevo a cabo con muestras de tejidos de raton a los que se habia inyectado previamente timidina marcada con tritio. Despues de inyectar al raton una dosis del orden de 1 {mu}c de timidina tritiada por gramo de peso corporal, se pudieron seguir durante un mes las transformaciones metabolicas de la timidina incorporada al acido desoxirribonucleico (DISIA) de los distintos tejidos. (author) [Russian] V literature uzhe soobshchalos' o sposobakh izmereniya kolichestva tritiya v tkanyakh zhivotnykh i v izolirovannykh srezakh tkanej. Odnako pri vsekh opisyvaemykh rabotakh dlya otscheta impul'sov obraztsov primenyalsya zhidkostnyj stsintillyatsionnyj spektrometr sovpadenij. Pri vypolnenii nastoyashchej raboty dlya izmerenij byl ispol'zovan zhidkostnyj stsintillyatsionnyj schetchik s odnim fotoehlementom. Issledovalis' glavnym obrazom neposredstvennoe rastvorenie tkanej zhivotnykh v giamine 10-KH i otschet impul'sov vzvesi liofilirovannoj tkani v stsintilliruyushchikh gelyakh. Razlichnye tkani, kak naprimer, pechen', kishechnik, krov' i kozha mogut byt' neposredstvenno rastvoreny v giamine i aktivnost' ikh izmerena s tochnost'yu do 10-15 protsentov. Budut obsuzhdeny metody vvedeniya popravki na gashenie i nekotorye yavleniya fosforestsentsii, poskol'ku oni vliyayut na otschet impul'sov v schetnom ustrojstve s edinym fotoehlementom. KHotya chuvstvitel'nost' ehtogo metoda predstavlyaetsya neskol'ko men'shej, chem pri ispol'zovanii schetchika sovpadenij, odnako ehtot metod udovletvoritelen dlya mnogikh vidov primenenij. Predstavlyaemaya rabota byla provedena na obraztsakh tkanej myshi posle predvaritel'noj in{sup e}ktsii mechennogo tritiem timidina. Pri vpryskivanii v mysh' dozy priblizitel'no v 1 mikrokyuri tritirovannogo timidina na gramm vesa ee tela, metabolicheskaya sud'ba timidina, pogloshchennogo deoksiribonukleinovoj kislotoj razlichnykh tkanej, mogla byt' proslezhena v techenie tselogo mesyatsa. (author)}
place = {IAEA}
year = {1962}
month = {Jan}
}