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Probing genetic diversity to characterize red rot resistance in sugarcane

Journal Article:

Abstract

Genetic diversity was assessed in a set of twelve sugarcane genotypes using Random Amplified Polymorphic DNA (RAPD). A total of thirty-two oligo-primers were employed, sixteen of them revealed amplification at 149 loci, out of which 136 were polymorphic. The genotype SPSG-26 showed the highest number of polymorphic loci, followed by CSSG-668 and HSF-242. Pairwise genetic similarity ranged from 67.2% to 83.3%. The UPGMA cluster analysis resolved most of the accessions in two groups. The clustering pattern did not place all resistant varieties in one or related group which depict diverse resistance source in the present set of sugarcane varieties. Ten primers revealed genotype specific bands among which four primers (K07, H02, K10 and F01) produced multiple genotype specific bands that aid genotype identification especially those with red rot resistance. The present study not only provided information on the genetic diversity among the genotypes but also revealed the potential of RAPD-PCR markers for genotype identification and therefore could be utilized in marker assisted selection for red rot resistance in sugarcane. (author)
Publication Date:
Oct 15, 2011
Product Type:
Journal Article
Resource Relation:
Journal Name: Pakistan Journal of Botany; Journal Volume: 43; Journal Issue: 5
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; DISEASE RESISTANCE; GENETIC VARIABILITY; GENOTYPE; SUGAR CANE; BIOLOGICAL VARIABILITY; GRAMINEAE; LILIOPSIDA; MAGNOLIOPHYTA; PLANTS; REEDS
OSTI ID:
21535084
Country of Origin:
Pakistan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0556-3321; TRN: PK1200100014021
Submitting Site:
INIS
Size:
page(s) 2513-2517
Announcement Date:
Mar 17, 2012

Journal Article:

Citation Formats

Mumtaz, A S, Dure-e-Nayab,, Iqbal, M J, and Shinwari, Z.K., E-mail: asmumtaz@qau.edu.pk. Probing genetic diversity to characterize red rot resistance in sugarcane. Pakistan: N. p., 2011. Web.
Mumtaz, A S, Dure-e-Nayab,, Iqbal, M J, & Shinwari, Z.K., E-mail: asmumtaz@qau.edu.pk. Probing genetic diversity to characterize red rot resistance in sugarcane. Pakistan.
Mumtaz, A S, Dure-e-Nayab,, Iqbal, M J, and Shinwari, Z.K., E-mail: asmumtaz@qau.edu.pk. 2011. "Probing genetic diversity to characterize red rot resistance in sugarcane." Pakistan.
@misc{etde_21535084,
title = {Probing genetic diversity to characterize red rot resistance in sugarcane}
author = {Mumtaz, A S, Dure-e-Nayab,, Iqbal, M J, and Shinwari, Z.K., E-mail: asmumtaz@qau.edu.pk}
abstractNote = {Genetic diversity was assessed in a set of twelve sugarcane genotypes using Random Amplified Polymorphic DNA (RAPD). A total of thirty-two oligo-primers were employed, sixteen of them revealed amplification at 149 loci, out of which 136 were polymorphic. The genotype SPSG-26 showed the highest number of polymorphic loci, followed by CSSG-668 and HSF-242. Pairwise genetic similarity ranged from 67.2% to 83.3%. The UPGMA cluster analysis resolved most of the accessions in two groups. The clustering pattern did not place all resistant varieties in one or related group which depict diverse resistance source in the present set of sugarcane varieties. Ten primers revealed genotype specific bands among which four primers (K07, H02, K10 and F01) produced multiple genotype specific bands that aid genotype identification especially those with red rot resistance. The present study not only provided information on the genetic diversity among the genotypes but also revealed the potential of RAPD-PCR markers for genotype identification and therefore could be utilized in marker assisted selection for red rot resistance in sugarcane. (author)}
journal = {Pakistan Journal of Botany}
issue = {5}
volume = {43}
place = {Pakistan}
year = {2011}
month = {Oct}
}