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Evaluation of Fe and Zn/Cu ratio in serum of patients with sickle cell anemia by total reflection X-ray fluorescence using synchrotron radiation

Abstract

Sickle cell anemia (SCA) is a blood disorder that affects hemoglobin, the protein found in red blood cells that help carry oxygen throughout the body. In this work we have analyzed serum samples from patients with SCA by using total reflection X-ray fluorescence using synchrotron radiation (SRTXRF). The SRTXRF measurements were performed at the X-ray fluorescence beamline at Brazilian National Synchrotron Light Laboratory (LNLS), in Campinas, Sao Paulo using a polychromatic beam. We have studied forty-three patients aged 18-50 years old, suffering from SCA and Sixty healthy volunteers aged 18-60 years old. It was possible to determine the concentrations of the following elements: P, S, Cl, K, Ca, Fe, Cu, Zn, Br and Rb. Student's t-test was applied in order to check whether the two populations (CG x SCA) had the same mean values. It was observed that elemental concentration of P, Cl, K, Fe, Cu, Zn and Br differed significantly ({alpha} = 0.05) between groups of healthy subjects and SCA. The concentrations of K, Fe and Cu in the serum samples of patients with SCA were larger 15%, 120 % and 20 %, respectively, when compared with the CG. On the other hand, the concentrations of P (-20 %),  More>>
Authors:
Canellas, Catarine G.L.; Leitao, Roberta G; Lopes, Ricardo T., E-mail: catarine@lin.ufrj.b, E-mail: ricardo@lin.ufrj.b; [1]  Carvalho, Silvia M.F., E-mail: silvia@hemorio.rj.gov.b; [2]  Bellido, Alfredo Victor B., E-mail: alfredo@ien.gov.b; [3]  Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.b [4] 
  1. Universidade Federal do Rio de Janeiro (PEN/COPPE/UFRJ), RJ (Brazil). Coordenacao dos Programas de Pos-Graduacao de Engenharia. Programa de Engenharia Nuclear. Lab. de Instrumentaco Nuclear
  2. State Institute of Hematology Arthur de Siqueira Cavalcanti (HEMORIO), Rio de Janeiro, RJ (Brazil)
  3. Federal Fluminense University (UFF), Niteroi, RJ (Brazil). Chemistry Inst.
  4. State University of Rio de Janeiro (UERJ), RJ (Brazil). Physics Inst.
Publication Date:
Jul 01, 2011
Product Type:
Conference
Report Number:
INIS-BR-10588
Resource Relation:
Conference: INAC 2011: International nuclear atlantic conference. Nuclear energy: new jobs for a better life;17. ENFIR: Meeting on nuclear reactor physics and thermal hydraulics;10. ENAN: Meeting on nuclear applications;2. ENIN: Meeting on nuclear industry, Belo Horizonte, MG (Brazil);Belo Horizonte, MG (Brazil);Belo Horizonte, MG (Brazil);Belo Horizonte, MG (Brazil), 24-28 Oct 2011;24-28 Oct 2011;24-28 Oct 2011;24-28 Oct 2011
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BLOOD SERUM; BRAZIL; COPPER; ERYTHROCYTES; IRON; LNLS STORAGE RING; SICKLE CELL ANEMIA; SYNCHROTRON RADIATION; X RADIATION; X-RAY FLUORESCENCE ANALYSIS; ZINC; ANEMIAS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BLOOD PLASMA; BODY FLUIDS; BREMSSTRAHLUNG; CHEMICAL ANALYSIS; DEVELOPING COUNTRIES; DISEASES; ELECTROMAGNETIC RADIATION; ELEMENTS; HEMIC DISEASES; IONIZING RADIATIONS; LATIN AMERICA; MATERIALS; METALS; NONDESTRUCTIVE ANALYSIS; RADIATION SOURCES; RADIATIONS; SOUTH AMERICA; STORAGE RINGS; SYMPTOMS; SYNCHROTRON RADIATION SOURCES; TRANSITION ELEMENTS; X-RAY EMISSION ANALYSIS
OSTI ID:
21518640
Research Organizations:
Associacao Brasileira de Energia Nuclear, Rio de Janeiro, RJ (Brazil)
Country of Origin:
Brazil
Language:
English
Other Identifying Numbers:
TRN: BR11V3644109423
Availability:
Available from INIS in electronic form
Submitting Site:
BRN
Size:
9 pages
Announcement Date:
Dec 29, 2011

Citation Formats

Canellas, Catarine G.L., Leitao, Roberta G, Lopes, Ricardo T., E-mail: catarine@lin.ufrj.b, E-mail: ricardo@lin.ufrj.b, Carvalho, Silvia M.F., E-mail: silvia@hemorio.rj.gov.b, Bellido, Alfredo Victor B., E-mail: alfredo@ien.gov.b, and Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.b. Evaluation of Fe and Zn/Cu ratio in serum of patients with sickle cell anemia by total reflection X-ray fluorescence using synchrotron radiation. Brazil: N. p., 2011. Web.
Canellas, Catarine G.L., Leitao, Roberta G, Lopes, Ricardo T., E-mail: catarine@lin.ufrj.b, E-mail: ricardo@lin.ufrj.b, Carvalho, Silvia M.F., E-mail: silvia@hemorio.rj.gov.b, Bellido, Alfredo Victor B., E-mail: alfredo@ien.gov.b, & Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.b. Evaluation of Fe and Zn/Cu ratio in serum of patients with sickle cell anemia by total reflection X-ray fluorescence using synchrotron radiation. Brazil.
Canellas, Catarine G.L., Leitao, Roberta G, Lopes, Ricardo T., E-mail: catarine@lin.ufrj.b, E-mail: ricardo@lin.ufrj.b, Carvalho, Silvia M.F., E-mail: silvia@hemorio.rj.gov.b, Bellido, Alfredo Victor B., E-mail: alfredo@ien.gov.b, and Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.b. 2011. "Evaluation of Fe and Zn/Cu ratio in serum of patients with sickle cell anemia by total reflection X-ray fluorescence using synchrotron radiation." Brazil.
@misc{etde_21518640,
title = {Evaluation of Fe and Zn/Cu ratio in serum of patients with sickle cell anemia by total reflection X-ray fluorescence using synchrotron radiation}
author = {Canellas, Catarine G.L., Leitao, Roberta G, Lopes, Ricardo T., E-mail: catarine@lin.ufrj.b, E-mail: ricardo@lin.ufrj.b, Carvalho, Silvia M.F., E-mail: silvia@hemorio.rj.gov.b, Bellido, Alfredo Victor B., E-mail: alfredo@ien.gov.b, and Anjos, Marcelino J., E-mail: marcelin@lin.ufrj.b}
abstractNote = {Sickle cell anemia (SCA) is a blood disorder that affects hemoglobin, the protein found in red blood cells that help carry oxygen throughout the body. In this work we have analyzed serum samples from patients with SCA by using total reflection X-ray fluorescence using synchrotron radiation (SRTXRF). The SRTXRF measurements were performed at the X-ray fluorescence beamline at Brazilian National Synchrotron Light Laboratory (LNLS), in Campinas, Sao Paulo using a polychromatic beam. We have studied forty-three patients aged 18-50 years old, suffering from SCA and Sixty healthy volunteers aged 18-60 years old. It was possible to determine the concentrations of the following elements: P, S, Cl, K, Ca, Fe, Cu, Zn, Br and Rb. Student's t-test was applied in order to check whether the two populations (CG x SCA) had the same mean values. It was observed that elemental concentration of P, Cl, K, Fe, Cu, Zn and Br differed significantly ({alpha} = 0.05) between groups of healthy subjects and SCA. The concentrations of K, Fe and Cu in the serum samples of patients with SCA were larger 15%, 120 % and 20 %, respectively, when compared with the CG. On the other hand, the concentrations of P (-20 %), Cl (-6 %), Zn (-25 %) and Br (-22 %) were smaller than the values determined for the control group. The serum level Cu/Zn ratio was significantly higher (60%) in the serum samples of patients with SCA group than the CG. So, the Cu/Zn ratio can be used as an adjuvant index in enhancement for diagnosis of SCA. There are evidences of an association among Fe, Cu, Zn and Cu/Zn in the SCA pathogenesis process. (author)}
place = {Brazil}
year = {2011}
month = {Jul}
}