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Genetic diversity of sesame (sesamum indicum L.) germplasm from Pakistan using RAPD markers

Journal Article:

Abstract

Genetic diversity among 20 sesame (Sesamum indicum L.) accessions was examined at DNA level by means of random amplified polymorphic DNA (RAPD) analysis. Ten primers used produced a total of 93 RAPD fragments, of which 70 (75%) were polymorphic. Each primer generated 5 to 17 amplified fragments with an average of 9.3 bands per primer. Based on pair-wise comparisons of RAPD amplification products, Nei and Li's similarity coefficients were computed to assess the associations among the accessions. Pair-wise similarity indices varied from 0.65 to 0.91. A UPGMA cluster analysis based on these genetic similarities located most of the accessions far apart from one another, showing a high level of polymorphism. Genetically, all the genotypes were classified into two major groups and six subgroups or clusters. A single accession (22243) was relatively distinct from rest of the accessions and created independent cluster. In conclusion, even with the use of a limited set of primers, RAPD technique revealed a high level of genetic variation among sesame accessions collected from diverse ecologies of Pakistan. This high level of genetic diversity among the genotypes suggested that RAPD technique is valuable for sesame systematic, and can be helpful for the upholding of germplasm banks and  More>>
Publication Date:
Aug 15, 2011
Product Type:
Journal Article
Resource Relation:
Journal Name: Pakistan Journal of Botany; Journal Volume: 43; Journal Issue: 4
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL MARKERS; DNA; GENETIC VARIABILITY; GENOTYPE; PAKISTAN; PLANT BREEDING; SESAMUM INDICUM; ASIA; BIOLOGICAL VARIABILITY; DEVELOPING COUNTRIES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS
OSTI ID:
21502172
Country of Origin:
Pakistan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0556-3321; TRN: PK1100634093985
Submitting Site:
INIS
Size:
page(s) 2153-2160
Announcement Date:
Nov 11, 2011

Journal Article:

Citation Formats

Akbar, F, Rabbani, M A, Masood, M S, and Shinwari, Z.K., E-mail: shinwari@qau.edu.p. Genetic diversity of sesame (sesamum indicum L.) germplasm from Pakistan using RAPD markers. Pakistan: N. p., 2011. Web.
Akbar, F, Rabbani, M A, Masood, M S, & Shinwari, Z.K., E-mail: shinwari@qau.edu.p. Genetic diversity of sesame (sesamum indicum L.) germplasm from Pakistan using RAPD markers. Pakistan.
Akbar, F, Rabbani, M A, Masood, M S, and Shinwari, Z.K., E-mail: shinwari@qau.edu.p. 2011. "Genetic diversity of sesame (sesamum indicum L.) germplasm from Pakistan using RAPD markers." Pakistan.
@misc{etde_21502172,
title = {Genetic diversity of sesame (sesamum indicum L.) germplasm from Pakistan using RAPD markers}
author = {Akbar, F, Rabbani, M A, Masood, M S, and Shinwari, Z.K., E-mail: shinwari@qau.edu.p}
abstractNote = {Genetic diversity among 20 sesame (Sesamum indicum L.) accessions was examined at DNA level by means of random amplified polymorphic DNA (RAPD) analysis. Ten primers used produced a total of 93 RAPD fragments, of which 70 (75%) were polymorphic. Each primer generated 5 to 17 amplified fragments with an average of 9.3 bands per primer. Based on pair-wise comparisons of RAPD amplification products, Nei and Li's similarity coefficients were computed to assess the associations among the accessions. Pair-wise similarity indices varied from 0.65 to 0.91. A UPGMA cluster analysis based on these genetic similarities located most of the accessions far apart from one another, showing a high level of polymorphism. Genetically, all the genotypes were classified into two major groups and six subgroups or clusters. A single accession (22243) was relatively distinct from rest of the accessions and created independent cluster. In conclusion, even with the use of a limited set of primers, RAPD technique revealed a high level of genetic variation among sesame accessions collected from diverse ecologies of Pakistan. This high level of genetic diversity among the genotypes suggested that RAPD technique is valuable for sesame systematic, and can be helpful for the upholding of germplasm banks and the competent choice of parents in breeding programs. (author)}
journal = {Pakistan Journal of Botany}
issue = {4}
volume = {43}
place = {Pakistan}
year = {2011}
month = {Aug}
}