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Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery

Abstract

In recent times self-assembled micellar nanoparticles have been successfully employed in tissue engineering for targeted drug delivery applications. In this review, silk sericin protein from non-mulberry Antheraea mylitta tropical tasar silk cocoons was blended with pluronic F-127 and F-87 in the presence of solvents to achieve self-assembled micellar nanostructures capable of carrying both hydrophilic (FITC-inulin) and hydrophobic (anticancer drug paclitaxel) drugs. The fabricated nanoparticles were subsequently characterized for their size distribution, drug loading capability, cellular uptake and cytotoxicity. Nanoparticle sizes ranged between 100 and 110 nm in diameter as confirmed by dynamic light scattering. Rapid uptake of these particles into cells was observed in in vitro cellular uptake studies using breast cancer MCF-7 cells. In vitro cytotoxicity assay using paclitaxel-loaded nanoparticles against breast cancer cells showed promising results comparable to free paclitaxel drugs. Drug-encapsulated nanoparticle-induced apoptosis in MCF-7 cells was confirmed by FACS and confocal microscopic studies using Annexin V staining. Up-regulation of pro-apoptotic protein Bax, down-regulation of anti-apoptotic protein Bcl-2 and cleavage of regulatory protein PARP through Western blot analysis suggested further drug-induced apoptosis in cells. This study projects silk sericin protein as an alternative natural biomaterial for fabrication of self-assembled nanoparticles in the presence of poloxamer for successful  More>>
Authors:
Mandal, Biman B; Kundu, S C, E-mail: kundu@hijli.iitkgp.ernet.i [1] 
  1. Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302 (India)
Publication Date:
Sep 02, 2009
Product Type:
Journal Article
Resource Relation:
Journal Name: Nanotechnology (Print); Journal Volume: 20; Journal Issue: 35; Other Information: DOI: 10.1088/0957-4484/20/35/355101; PII: S0957-4484(09)10672-4
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; ANTINEOPLASTIC DRUGS; APOPTOSIS; FABRICATION; INULIN; MAMMARY GLANDS; NANOSTRUCTURES; NEOPLASMS; PROTEINS; SOLVENTS; TOXICITY; BODY; CARBOHYDRATES; DISEASES; DRUGS; GLANDS; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; SACCHARIDES
OSTI ID:
21479385
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0957-4484; TRN: GB10P4205071498
Availability:
Available from http://dx.doi.org/10.1088/0957-4484/20/35/355101
Submitting Site:
GBN
Size:
14 pages
Announcement Date:
Sep 29, 2011

Citation Formats

Mandal, Biman B, and Kundu, S C, E-mail: kundu@hijli.iitkgp.ernet.i. Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery. United Kingdom: N. p., 2009. Web. doi:10.1088/0957-4484/20/35/355101.
Mandal, Biman B, & Kundu, S C, E-mail: kundu@hijli.iitkgp.ernet.i. Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery. United Kingdom. doi:10.1088/0957-4484/20/35/355101.
Mandal, Biman B, and Kundu, S C, E-mail: kundu@hijli.iitkgp.ernet.i. 2009. "Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery." United Kingdom. doi:10.1088/0957-4484/20/35/355101. https://www.osti.gov/servlets/purl/10.1088/0957-4484/20/35/355101.
@misc{etde_21479385,
title = {Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery}
author = {Mandal, Biman B, and Kundu, S C, E-mail: kundu@hijli.iitkgp.ernet.i}
abstractNote = {In recent times self-assembled micellar nanoparticles have been successfully employed in tissue engineering for targeted drug delivery applications. In this review, silk sericin protein from non-mulberry Antheraea mylitta tropical tasar silk cocoons was blended with pluronic F-127 and F-87 in the presence of solvents to achieve self-assembled micellar nanostructures capable of carrying both hydrophilic (FITC-inulin) and hydrophobic (anticancer drug paclitaxel) drugs. The fabricated nanoparticles were subsequently characterized for their size distribution, drug loading capability, cellular uptake and cytotoxicity. Nanoparticle sizes ranged between 100 and 110 nm in diameter as confirmed by dynamic light scattering. Rapid uptake of these particles into cells was observed in in vitro cellular uptake studies using breast cancer MCF-7 cells. In vitro cytotoxicity assay using paclitaxel-loaded nanoparticles against breast cancer cells showed promising results comparable to free paclitaxel drugs. Drug-encapsulated nanoparticle-induced apoptosis in MCF-7 cells was confirmed by FACS and confocal microscopic studies using Annexin V staining. Up-regulation of pro-apoptotic protein Bax, down-regulation of anti-apoptotic protein Bcl-2 and cleavage of regulatory protein PARP through Western blot analysis suggested further drug-induced apoptosis in cells. This study projects silk sericin protein as an alternative natural biomaterial for fabrication of self-assembled nanoparticles in the presence of poloxamer for successful delivery of both hydrophobic and hydrophilic drugs to target sites.}
doi = {10.1088/0957-4484/20/35/355101}
journal = {Nanotechnology (Print)}
issue = {35}
volume = {20}
place = {United Kingdom}
year = {2009}
month = {Sep}
}