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Protective effect of atorvastatin on radiation-induced vascular endothelial cell injury in vitro

Journal Article:

Abstract

Vascular endothelial cells are very sensitive to ionizing radiation, and it is important to develop effective prevent agents and measures in radiation exposure protection. In the present study, the protective effects of atorvastatin on irradiated human umbilical vein endothelial cells (HUVEC) and the possible mechanisms were explored. Cultured HUVEC were treated by atorvastatin at a final concentration of 10 {mu}mol/ml for 10 minutes, and then irradiated at a dose of 2 Gy or 25 Gy. Twenty-four hours after irradiation, apoptosis of HUVEC was monitored by flow cytometry, and the expression of thrombomodulin (TM) and protein C activation in HUVEC was respectively assessed by flow cytometry and spectrophotometry. After treatment with atorvastatin for 24 h, the rate of cell apoptosis decreased by 6% and 16% in cells irradiated with 2 Gy and 25 Gy, respectively. TM expression increased by 77%, 59%, and 61% in untreated cells, 2 Gy irradiation-treated cells, and 25 Gy irradiation-treated cells, respectively. The protein C levels in 2 Gy and 25 Gy irradiation-treated cells were reduced by 23% and 34% when compared with untreated cells, but up-regulated by 79% and 76% when compared with cells which were irradiated and treated with atorvastatin. In conclusion, these data indicate  More>>
Authors:
Xinze, Ran; Zhaowen, Zong; Dengqun, Liu; Yongping, Su; Huaien, Zheng; [1]  Xi, Ran; Guiming, Xiang [2] 
  1. College of Preventive Medicine, Third Military Medical Univ., Chongqing (China)
  2. Xinqiao Hospital, Third Military Medical Univ., Chongqing (China)
Publication Date:
Sep 15, 2010
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Radiation Research; Journal Volume: 51; Journal Issue: 5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL CELLS; APOPTOSIS; CELL CULTURES; CESIUM 137; GAMMA RADIATION; IN VITRO; RADIATION DOSES; RADIATION INJURIES; SIZE; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; CESIUM ISOTOPES; DISEASES; DOSES; ELECTROMAGNETIC RADIATION; INJURIES; INTERMEDIATE MASS NUCLEI; IONIZING RADIATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES
OSTI ID:
21449856
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0449-3060; TRN: JP1101060044753
Availability:
Available from http://dx.doi.org/10.1269/jrr.09119
Submitting Site:
INIS
Size:
page(s) 527-533
Announcement Date:
Jul 07, 2011

Journal Article:

Citation Formats

Xinze, Ran, Zhaowen, Zong, Dengqun, Liu, Yongping, Su, Huaien, Zheng, Xi, Ran, and Guiming, Xiang. Protective effect of atorvastatin on radiation-induced vascular endothelial cell injury in vitro. Japan: N. p., 2010. Web. doi:10.1269/JRR.09119.
Xinze, Ran, Zhaowen, Zong, Dengqun, Liu, Yongping, Su, Huaien, Zheng, Xi, Ran, & Guiming, Xiang. Protective effect of atorvastatin on radiation-induced vascular endothelial cell injury in vitro. Japan. doi:10.1269/JRR.09119.
Xinze, Ran, Zhaowen, Zong, Dengqun, Liu, Yongping, Su, Huaien, Zheng, Xi, Ran, and Guiming, Xiang. 2010. "Protective effect of atorvastatin on radiation-induced vascular endothelial cell injury in vitro." Japan. doi:10.1269/JRR.09119. https://www.osti.gov/servlets/purl/10.1269/JRR.09119.
@misc{etde_21449856,
title = {Protective effect of atorvastatin on radiation-induced vascular endothelial cell injury in vitro}
author = {Xinze, Ran, Zhaowen, Zong, Dengqun, Liu, Yongping, Su, Huaien, Zheng, Xi, Ran, and Guiming, Xiang}
abstractNote = {Vascular endothelial cells are very sensitive to ionizing radiation, and it is important to develop effective prevent agents and measures in radiation exposure protection. In the present study, the protective effects of atorvastatin on irradiated human umbilical vein endothelial cells (HUVEC) and the possible mechanisms were explored. Cultured HUVEC were treated by atorvastatin at a final concentration of 10 {mu}mol/ml for 10 minutes, and then irradiated at a dose of 2 Gy or 25 Gy. Twenty-four hours after irradiation, apoptosis of HUVEC was monitored by flow cytometry, and the expression of thrombomodulin (TM) and protein C activation in HUVEC was respectively assessed by flow cytometry and spectrophotometry. After treatment with atorvastatin for 24 h, the rate of cell apoptosis decreased by 6% and 16% in cells irradiated with 2 Gy and 25 Gy, respectively. TM expression increased by 77%, 59%, and 61% in untreated cells, 2 Gy irradiation-treated cells, and 25 Gy irradiation-treated cells, respectively. The protein C levels in 2 Gy and 25 Gy irradiation-treated cells were reduced by 23% and 34% when compared with untreated cells, but up-regulated by 79% and 76% when compared with cells which were irradiated and treated with atorvastatin. In conclusion, these data indicate that atorvastatin exerts protective effects on irradiated HUVEC by reducing apoptosis by up-regulating TM expression and enhancing protein C activation in irradiated HUVEC. (author)}
doi = {10.1269/JRR.09119}
journal = {Journal of Radiation Research}
issue = {5}
volume = {51}
place = {Japan}
year = {2010}
month = {Sep}
}