Abstract
Aim: To develop a simplified and fully automated synthesis procedure of 3'-deoxy-3'-[{sup 18}F]fluorothymidine ([{sup 18}F]FLT) using PET-MF-2V-IT-I synthesis module. Methods: Synthesis of [{sup 18}F]FLT was performed using PET-MF-2V-IT-I synthesis module by one-pot two-step reaction procedure, including nucleophilic fluorination of 3-N-t-butoxycarbonyl-1-[5'-O-(4,4'-dimethoxy triphenylmethyl)-2'-deoxy-3'-O-(4-nitrobenzenesulfonyl) -{beta}-D-threopentofuranosyl]thymine (15 mg) as the precursor molecule with [{sup 18}F]fluoride, and subsequent hydrolysis of the protecting group with 1.0 M HCl at the same reaction vessel and purification with SEP PAK cartridges instead of the HPLC system. Results: The automated synthesis of [{sup 18}F]FLT with SEP PAK purification gave corrected radiochemical yield of 23.2{+-}2.6% (n=6, uncorrected yield: 16-22%) and radiochemical purity of >97% within the total synthesis time of 35 min. Conclusion: The fully one-pot automated synthesis procedure with SEP PAK purification can be applied to the fully automated synthesis of [{sup 18}F]FLT using commercial [{sup 18}F]FDG synthesis module.
Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China);
Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)];
Xiaolan, Tang;
[1]
Fuhua, Wen;
[2]
Mingfang, Wang;
Baoyuan, Li
[3]
- College of Science, South China Agricultural University, Guangzhou 510642 (China)
- PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China)
- Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)
Citation Formats
Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China), Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)], Xiaolan, Tang, Fuhua, Wen, Mingfang, Wang, and Baoyuan, Li.
A facile and rapid automated synthesis of 3'-deoxy-3'-[{sup 18}F]fluorothymidine.
United Kingdom: N. p.,
2010.
Web.
doi:10.1016/j.apradiso.2010.04.010.
Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China), Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)], Xiaolan, Tang, Fuhua, Wen, Mingfang, Wang, & Baoyuan, Li.
A facile and rapid automated synthesis of 3'-deoxy-3'-[{sup 18}F]fluorothymidine.
United Kingdom.
https://doi.org/10.1016/j.apradiso.2010.04.010
Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China), Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)], Xiaolan, Tang, Fuhua, Wen, Mingfang, Wang, and Baoyuan, Li.
2010.
"A facile and rapid automated synthesis of 3'-deoxy-3'-[{sup 18}F]fluorothymidine."
United Kingdom.
https://doi.org/10.1016/j.apradiso.2010.04.010.
@misc{etde_21418577,
title = {A facile and rapid automated synthesis of 3'-deoxy-3'-[{sup 18}F]fluorothymidine}
author = {Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China), Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)], Xiaolan, Tang, Fuhua, Wen, Mingfang, Wang, and Baoyuan, Li}
abstractNote = {Aim: To develop a simplified and fully automated synthesis procedure of 3'-deoxy-3'-[{sup 18}F]fluorothymidine ([{sup 18}F]FLT) using PET-MF-2V-IT-I synthesis module. Methods: Synthesis of [{sup 18}F]FLT was performed using PET-MF-2V-IT-I synthesis module by one-pot two-step reaction procedure, including nucleophilic fluorination of 3-N-t-butoxycarbonyl-1-[5'-O-(4,4'-dimethoxy triphenylmethyl)-2'-deoxy-3'-O-(4-nitrobenzenesulfonyl) -{beta}-D-threopentofuranosyl]thymine (15 mg) as the precursor molecule with [{sup 18}F]fluoride, and subsequent hydrolysis of the protecting group with 1.0 M HCl at the same reaction vessel and purification with SEP PAK cartridges instead of the HPLC system. Results: The automated synthesis of [{sup 18}F]FLT with SEP PAK purification gave corrected radiochemical yield of 23.2{+-}2.6% (n=6, uncorrected yield: 16-22%) and radiochemical purity of >97% within the total synthesis time of 35 min. Conclusion: The fully one-pot automated synthesis procedure with SEP PAK purification can be applied to the fully automated synthesis of [{sup 18}F]FLT using commercial [{sup 18}F]FDG synthesis module.}
doi = {10.1016/j.apradiso.2010.04.010}
journal = []
issue = {9}
volume = {68}
place = {United Kingdom}
year = {2010}
month = {Sep}
}
title = {A facile and rapid automated synthesis of 3'-deoxy-3'-[{sup 18}F]fluorothymidine}
author = {Tang Ganghua, E-mail: gtang0224@yahoo.com.c [PET-CT Center, Department of Nuclear Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080 (China), Nan Fang PET Center, Nan Fang Hospital, Southern Medical University, Guangzhou 510515 (China)], Xiaolan, Tang, Fuhua, Wen, Mingfang, Wang, and Baoyuan, Li}
abstractNote = {Aim: To develop a simplified and fully automated synthesis procedure of 3'-deoxy-3'-[{sup 18}F]fluorothymidine ([{sup 18}F]FLT) using PET-MF-2V-IT-I synthesis module. Methods: Synthesis of [{sup 18}F]FLT was performed using PET-MF-2V-IT-I synthesis module by one-pot two-step reaction procedure, including nucleophilic fluorination of 3-N-t-butoxycarbonyl-1-[5'-O-(4,4'-dimethoxy triphenylmethyl)-2'-deoxy-3'-O-(4-nitrobenzenesulfonyl) -{beta}-D-threopentofuranosyl]thymine (15 mg) as the precursor molecule with [{sup 18}F]fluoride, and subsequent hydrolysis of the protecting group with 1.0 M HCl at the same reaction vessel and purification with SEP PAK cartridges instead of the HPLC system. Results: The automated synthesis of [{sup 18}F]FLT with SEP PAK purification gave corrected radiochemical yield of 23.2{+-}2.6% (n=6, uncorrected yield: 16-22%) and radiochemical purity of >97% within the total synthesis time of 35 min. Conclusion: The fully one-pot automated synthesis procedure with SEP PAK purification can be applied to the fully automated synthesis of [{sup 18}F]FLT using commercial [{sup 18}F]FDG synthesis module.}
doi = {10.1016/j.apradiso.2010.04.010}
journal = []
issue = {9}
volume = {68}
place = {United Kingdom}
year = {2010}
month = {Sep}
}