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Studies on the Chromatin Isolated from the Organs of Animals Received Whole-body X-ray Irradiation

Abstract

Within experimental chromatin, the total protein: DNA ratio did not vary in the same organs of control and irradiated rats. However, the amount of RNA and total protein associated with the DNA varied considerably among the different types of chromatin. In particular, the content of chromatin was the highest in the irradiated tissue, and the lowest in the chromatin control tissue. RNA and total protein ratio of chromatins from brain, liver, testis and spleen declined with experimental organs. 2) There was the same quantitative relationship between the amount of RNA and the amount histone-protein associated with DNA in each chromatin. 3) RNA:DNA ratio of chromatin showed a 1.5-2 times increase in the irradiated organs except brain. However, RNA:DNA ratio was decreased in chromatin by irradiation. 4) Histone-protein: Residual protein ratio was greatly varied among the organs. However, the effect was not found by irradiation. 5) Priming activity of chromatins showed a higher value in testis and the activity was greater in organs with higher metabolic activity. 6) Inhibition of Actinomycin D observable in chromatin for testis, liver, spleen and brain declined without relationship between irradiated and non-irradiated conditions. Ammonium sulfate in DNA of chromatin from histone showed increased priming activity  More>>
Authors:
Han, Su Nam [1] 
  1. Seoul National University College of Medicine, Seoul (Korea, Republic of)
Publication Date:
Sep 15, 1967
Product Type:
Journal Article
Resource Relation:
Journal Name: Korean Journal of Nuclear Medicine; Journal Volume: 1; Journal Issue: 2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; AMMONIA; ANIMALS; CHROMATIN; DNA; IRRADIATION; ORGANS; PROTEINS; RNA; BODY; HYDRIDES; HYDROGEN COMPOUNDS; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; NUCLEIC ACIDS; ORGANIC COMPOUNDS
OSTI ID:
21404565
Country of Origin:
Korea, Republic of
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1225-6714; TRN: KR1004285130517
Submitting Site:
KRN
Size:
page(s) 27-34
Announcement Date:
Mar 29, 2011

Citation Formats

Han, Su Nam. Studies on the Chromatin Isolated from the Organs of Animals Received Whole-body X-ray Irradiation. Korea, Republic of: N. p., 1967. Web.
Han, Su Nam. Studies on the Chromatin Isolated from the Organs of Animals Received Whole-body X-ray Irradiation. Korea, Republic of.
Han, Su Nam. 1967. "Studies on the Chromatin Isolated from the Organs of Animals Received Whole-body X-ray Irradiation." Korea, Republic of.
@misc{etde_21404565,
title = {Studies on the Chromatin Isolated from the Organs of Animals Received Whole-body X-ray Irradiation}
author = {Han, Su Nam}
abstractNote = {Within experimental chromatin, the total protein: DNA ratio did not vary in the same organs of control and irradiated rats. However, the amount of RNA and total protein associated with the DNA varied considerably among the different types of chromatin. In particular, the content of chromatin was the highest in the irradiated tissue, and the lowest in the chromatin control tissue. RNA and total protein ratio of chromatins from brain, liver, testis and spleen declined with experimental organs. 2) There was the same quantitative relationship between the amount of RNA and the amount histone-protein associated with DNA in each chromatin. 3) RNA:DNA ratio of chromatin showed a 1.5-2 times increase in the irradiated organs except brain. However, RNA:DNA ratio was decreased in chromatin by irradiation. 4) Histone-protein: Residual protein ratio was greatly varied among the organs. However, the effect was not found by irradiation. 5) Priming activity of chromatins showed a higher value in testis and the activity was greater in organs with higher metabolic activity. 6) Inhibition of Actinomycin D observable in chromatin for testis, liver, spleen and brain declined without relationship between irradiated and non-irradiated conditions. Ammonium sulfate in DNA of chromatin from histone showed increased priming activity with dissociation by Electrostatics. It may give different effect of ammonium sulfate on stimulation by property of chromatins. 7) It is suggested that the results support a proposal that the higher sensitivity of radioactive in testis, spleen by irradiated showed a increase and decrease lower-sensitivity of radioactive from brain, liver than did priming activity under the radioactive conditions.}
journal = []
issue = {2}
volume = {1}
place = {Korea, Republic of}
year = {1967}
month = {Sep}
}