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{sup 125}I Labelling of Protein Using Immobilized Enzyme

Journal Article:

Abstract

For an effective solid-phase labelling of protein with {sup 125}I, studies on the immobilization of lactoperoxidase (LPO) on the inner wall of polystyrene tubes were carried out. Labelling of bovine serum albumin (BSA) and insulin was also practiced using the LPO immobilized tubes. The immobilized enzyme of about 2.5 mu g/tube was sufficient for small scale labelling since the results of radio-paper chromatography of the labelling mixture of insulin indicated that the yields were sufficiently high (80%) even in the reactions conducted at room temperature for 60 sec. The results of the Sephadex column chromatography indicated that the labelled products were not contaminated with LPO-{sup 125}I, and the radiochemical purity of the products was more than 90%. In considering the general trend that the {sup 125}I labelled protein obtained by using LPO maintains its intactness better than those obtained by using chloramine-T, together with the tendency of yield enhancing with increase of reactants-concentration, the LPO immobilized tube method is estimated to be one of the simple methods of labelling. The product might be applicable without further purification.
Authors:
Kim, Jae Rok; Park, Kyung Bae; Awh, Ok Doo [1] 
  1. Korea Advanced Energy Research Institute, Daejeon (Korea, Republic of)
Publication Date:
Mar 15, 1984
Product Type:
Journal Article
Resource Relation:
Journal Name: Korean Journal of Nuclear Medicine; Journal Volume: 18; Journal Issue: 1
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; CHROMATOGRAPHY; IMMOBILIZED ENZYMES; INSULIN; IODINE 125; POLYSTYRENE; PROTEINS; RADIOCHEMISTRY; TUBES; BETA DECAY RADIOISOTOPES; CHEMISTRY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PEPTIDE HORMONES; PETROCHEMICALS; PETROLEUM PRODUCTS; PLASTICS; POLYMERS; POLYOLEFINS; POLYVINYLS; RADIOISOTOPES; SEPARATION PROCESSES; SYNTHETIC MATERIALS
OSTI ID:
21370216
Country of Origin:
Korea, Republic of
Language:
Korean
Other Identifying Numbers:
Journal ID: ISSN 1225-6714; TRN: KR1003218100670
Submitting Site:
KRN
Size:
page(s) 55-62
Announcement Date:
Dec 22, 2010

Journal Article:

Citation Formats

Kim, Jae Rok, Park, Kyung Bae, and Awh, Ok Doo. {sup 125}I Labelling of Protein Using Immobilized Enzyme. Korea, Republic of: N. p., 1984. Web.
Kim, Jae Rok, Park, Kyung Bae, & Awh, Ok Doo. {sup 125}I Labelling of Protein Using Immobilized Enzyme. Korea, Republic of.
Kim, Jae Rok, Park, Kyung Bae, and Awh, Ok Doo. 1984. "{sup 125}I Labelling of Protein Using Immobilized Enzyme." Korea, Republic of.
@misc{etde_21370216,
title = {{sup 125}I Labelling of Protein Using Immobilized Enzyme}
author = {Kim, Jae Rok, Park, Kyung Bae, and Awh, Ok Doo}
abstractNote = {For an effective solid-phase labelling of protein with {sup 125}I, studies on the immobilization of lactoperoxidase (LPO) on the inner wall of polystyrene tubes were carried out. Labelling of bovine serum albumin (BSA) and insulin was also practiced using the LPO immobilized tubes. The immobilized enzyme of about 2.5 mu g/tube was sufficient for small scale labelling since the results of radio-paper chromatography of the labelling mixture of insulin indicated that the yields were sufficiently high (80%) even in the reactions conducted at room temperature for 60 sec. The results of the Sephadex column chromatography indicated that the labelled products were not contaminated with LPO-{sup 125}I, and the radiochemical purity of the products was more than 90%. In considering the general trend that the {sup 125}I labelled protein obtained by using LPO maintains its intactness better than those obtained by using chloramine-T, together with the tendency of yield enhancing with increase of reactants-concentration, the LPO immobilized tube method is estimated to be one of the simple methods of labelling. The product might be applicable without further purification.}
journal = {Korean Journal of Nuclear Medicine}
issue = {1}
volume = {18}
place = {Korea, Republic of}
year = {1984}
month = {Mar}
}