Abstract
Bioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET{sub 50}) was adopted as the endpoint. Paralysis of swimming movements was observed between approx0.5-3 h of exposure to lake water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity within 24 h after being placed in control water. The same effects were observed in bioassays with a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression analysis showed significant relationships between ET{sub 50}vs. cell density, biomass and saxitoxins content, suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins in natural samples, with important advantages over mouse bioassays. - A new Daphnia bioassay, as an alternative to the mouse bioassay, is able to detect effects of fast-acting, potent neurotoxins in raw water.
Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b;
[1]
Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co;
[2]
Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b;
[3]
Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b
[3]
- Laboratorio de Avaliacao e Promocao da Saude Ambiental, Departamento de Biologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ 21045-900 (Brazil)
- Departamento de Engenharia Sanitaria e Ambiental Faculdade de Engenharia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG 36036-900 (Brazil)
- Laboratorio de Ecofisiologia e Toxicologia de Cianobacterias, Instituto de Biofisica Carlos Chagas Filho, CCS, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, RJ 21949-900 (Brazil)
Citation Formats
Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b, Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co, Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b, and Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b.
A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test.
United Kingdom: N. p.,
2010.
Web.
doi:10.1016/j.envpol.2010.03.007.
Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b, Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co, Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b, & Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b.
A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test.
United Kingdom.
https://doi.org/10.1016/j.envpol.2010.03.007
Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b, Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co, Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b, and Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b.
2010.
"A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test."
United Kingdom.
https://doi.org/10.1016/j.envpol.2010.03.007.
@misc{etde_21358713,
title = {A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test}
author = {Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b, Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co, Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b, and Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b}
abstractNote = {Bioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET{sub 50}) was adopted as the endpoint. Paralysis of swimming movements was observed between approx0.5-3 h of exposure to lake water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity within 24 h after being placed in control water. The same effects were observed in bioassays with a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression analysis showed significant relationships between ET{sub 50}vs. cell density, biomass and saxitoxins content, suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins in natural samples, with important advantages over mouse bioassays. - A new Daphnia bioassay, as an alternative to the mouse bioassay, is able to detect effects of fast-acting, potent neurotoxins in raw water.}
doi = {10.1016/j.envpol.2010.03.007}
journal = []
issue = {6}
volume = {158}
place = {United Kingdom}
year = {2010}
month = {Jun}
}
title = {A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test}
author = {Ferrao-Filho, Aloysio da S., E-mail: aloysio@ioc.fiocruz.b, Soares, Maria Carolina S., E-mail: mcarolsoares@gmail.co, Freitas de Magalhaes, Valeria, E-mail: valeria@biof.ufrj.b, and Azevedo, Sandra M.F.O., E-mail: sazevedo@biof.ufrj.b}
abstractNote = {Bioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET{sub 50}) was adopted as the endpoint. Paralysis of swimming movements was observed between approx0.5-3 h of exposure to lake water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity within 24 h after being placed in control water. The same effects were observed in bioassays with a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression analysis showed significant relationships between ET{sub 50}vs. cell density, biomass and saxitoxins content, suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins in natural samples, with important advantages over mouse bioassays. - A new Daphnia bioassay, as an alternative to the mouse bioassay, is able to detect effects of fast-acting, potent neurotoxins in raw water.}
doi = {10.1016/j.envpol.2010.03.007}
journal = []
issue = {6}
volume = {158}
place = {United Kingdom}
year = {2010}
month = {Jun}
}